FastQCFastQC Report
Mon 6 Jun 2016
ERR523020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7250409
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT312820.431451522252055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289190.3988602574006515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262780.36243472609614164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159390.21983587408655153No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118830.16389420238223804No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA95060.13110984497564207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG120700.053.5376852
GTACCTG144100.045.3527571
ACCTGGG140200.045.18313
CCTGGGG123350.033.792374
TATAACG7400.026.0385192
CTGGGGG81050.022.5543735
TAACGCA9450.022.3776744
CATGGGG197400.020.9255054
TATCACG6400.020.5609042
TGGGGGG124700.020.3495836
ATGGGGG115100.020.169075
GAGTACT278550.019.85259612-13
GTACATG407200.019.8366471
ATAACGC10700.019.7634583
GTACACG17300.019.5676771
TACATGG419050.019.0654582
TACCGGG9750.018.7985422
ACATGGG414950.018.573013
AGTACTT291600.017.97280712-13
GGGTTAG19350.017.4946161
Produced by FastQC (version 0.11.5)