##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523020_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7250409
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14580225750023	33.0	31.0	34.0	30.0	34.0
2	32.275475218018734	34.0	31.0	34.0	30.0	34.0
3	32.45964482831244	34.0	31.0	34.0	30.0	35.0
4	35.56057003129065	37.0	35.0	37.0	33.0	37.0
5	35.502377865855564	36.0	35.0	37.0	33.0	37.0
6	35.634164914006924	37.0	35.0	37.0	33.0	37.0
7	35.48119726763001	36.0	35.0	37.0	33.0	37.0
8	35.471582775537215	36.0	35.0	37.0	33.0	37.0
9	36.73436850252172	37.0	37.0	39.0	33.0	39.0
10-11	36.53887739298569	37.5	35.0	39.0	33.0	39.0
12-13	36.54772227332279	37.5	35.0	39.0	33.0	39.0
14-15	37.678217463318276	38.0	37.0	41.0	33.0	41.0
16-17	37.50082574651996	38.0	36.5	41.0	32.5	41.0
18-19	37.40885052139817	38.0	36.0	41.0	32.0	41.0
20-21	37.42879105164964	38.0	36.0	41.0	33.0	41.0
22-23	37.27488587195563	38.0	36.0	40.0	32.5	41.0
24-25	37.19025719790429	38.0	36.5	40.0	32.0	41.0
26-27	36.86624278713104	38.0	36.0	40.0	32.0	41.0
28-29	36.82075362920905	38.0	35.5	40.0	32.0	41.0
30-31	36.57391651974392	37.5	35.5	40.0	31.0	41.0
32-33	36.50547113962812	37.5	36.0	40.0	30.5	41.0
34-35	36.25518229385404	37.5	35.0	40.0	30.5	41.0
36-37	36.022426252091435	37.0	35.0	40.0	30.0	41.0
38-39	35.97178828118524	37.0	35.0	40.0	30.0	41.0
40-41	36.161407790926006	37.5	35.0	40.0	30.0	41.0
42-43	36.190993639117465	37.5	35.0	40.0	30.0	41.0
44-45	36.00608420849086	37.0	35.0	40.0	30.0	41.0
46-47	35.8780972218257	37.0	35.0	40.0	29.0	41.0
48-49	35.641591667449376	37.5	34.5	40.0	28.5	41.0
50-51	35.46184456628585	37.5	34.0	40.0	27.5	41.0
52-53	35.46905222863979	37.5	35.0	40.0	27.5	41.0
54-55	35.2757814766036	37.5	34.0	40.0	27.0	41.0
56-57	35.02417118813573	37.0	34.5	40.0	26.5	41.0
58-59	34.83317706628688	37.0	34.0	39.5	26.5	41.0
60-61	34.62554015918275	36.5	33.5	39.0	26.0	41.0
62-63	34.39138805824609	36.5	33.5	39.0	26.0	41.0
64-65	34.204860167198845	36.5	33.0	38.5	25.0	40.0
66-67	33.69984672588815	35.5	33.0	38.0	25.0	40.0
68-69	33.554571404178716	35.5	33.0	37.0	23.5	40.0
70-71	33.12608019768264	35.0	32.5	37.0	24.0	39.0
72-73	32.889295762487336	35.0	32.0	37.0	23.5	39.0
74-75	32.69355708071089	35.0	32.0	37.0	22.5	39.0
76-77	31.136624844198444	34.0	29.5	36.0	21.0	37.0
78-79	32.25591211475104	35.0	31.5	36.5	23.0	37.0
80-81	32.36111693836858	35.0	32.0	36.0	23.0	37.5
82-83	32.26934480523788	35.0	32.0	37.0	23.5	37.0
84-85	31.933861317340856	35.0	31.5	35.5	22.0	36.5
86-87	31.825457088007035	35.0	31.0	36.0	22.0	36.0
88-89	31.771734049761882	35.0	31.0	36.0	22.0	37.0
90-91	31.529793353726667	34.5	31.0	35.0	21.5	36.5
92-93	31.315984587903937	34.0	31.0	35.0	21.0	36.5
94-95	31.43608277822672	34.0	31.0	35.0	20.5	36.5
96-97	31.1632847195241	34.0	31.0	35.0	20.0	37.0
98-99	31.015995856233765	34.0	30.0	35.0	20.0	36.0
100	29.78936291731956	34.0	29.0	35.0	18.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.09104390970896503
1101	2	-0.16274320993510827
1101	3	-0.12910060249036093
1101	4	-0.14458201130184278
1101	5	-0.24215926279713784
1101	6	-0.13097468203588392
1101	7	-0.045965914190269075
1101	8	-0.16853919892564306
1101	9	-0.158075699902831
1101	10-11	-0.14249804218189155
1101	12-13	-0.1608808233987986
1101	14-15	-0.3833338595242921
1101	16-17	-0.34782340042570326
1101	18-19	-0.4251954967455802
1101	20-21	-0.32597653550861594
1101	22-23	-0.8037888659669221
1101	24-25	-0.3237808587045521
1101	26-27	-0.32473491543025546
1101	28-29	-0.3631528479113797
1101	30-31	-0.4107778509666602
1101	32-33	-0.3307249474394567
1101	34-35	-0.4042654386685669
1101	36-37	-0.43426191297208305
1101	38-39	-0.31354030904304153
1101	40-41	-0.2740055218556421
1101	42-43	-0.37036110452464044
1101	44-45	-0.4628826552987917
1101	46-47	-0.3134748057418122
1101	48-49	-0.33897529469300736
1101	50-51	-0.39203687764624817
1101	52-53	-0.38951123835678203
1101	54-55	-0.38570556911998466
1101	56-57	-0.49025370871743235
1101	58-59	-0.4414619776391149
1101	60-61	-0.5971874690846093
1101	62-63	-0.49265010863891945
1101	64-65	-0.5095477549684162
1101	66-67	-0.37894991415976875
1101	68-69	-0.5329705225148871
1101	70-71	-0.5622989874753443
1101	72-73	-0.5985830834157753
1101	74-75	-0.4531871322207479
1101	76-77	-0.6058793609548374
1101	78-79	-0.5191374156310289
1101	80-81	-0.4607759153715847
1101	82-83	-0.38144542604747755
1101	84-85	-0.4146057363651856
1101	86-87	-0.4880358051818199
1101	88-89	-0.5017165099823764
1101	90-91	-0.5172688558192817
1101	92-93	-0.5317699699416387
1101	94-95	-0.5670260184654161
1101	96-97	-0.6162667159851587
1101	98-99	-0.5808920799203641
1101	100	-0.4720340946727717
1102	1	-0.05548528105092032
1102	2	-0.15282031941318763
1102	3	-0.18832120161206944
1102	4	-0.08365634215338247
1102	5	-0.10925845369307297
1102	6	-0.16058263686834806
1102	7	-0.07524601840480472
1102	8	-0.12126079509167198
1102	9	-0.14664066525103436
1102	10-11	-0.26116661737518854
1102	12-13	-0.18630329409092639
1102	14-15	-0.23480037606252324
1102	16-17	-0.2753114410768731
1102	18-19	-0.23771519040722922
1102	20-21	-0.33000778177041923
1102	22-23	-0.28902182830663037
1102	24-25	-0.2036979598041384
1102	26-27	-0.1922715421443968
1102	28-29	-0.27493556876051883
1102	30-31	-0.38541272328098586
1102	32-33	-0.23145684000711952
1102	34-35	-0.2597576481645447
1102	36-37	-0.31918068993542903
1102	38-39	-0.3263266888202665
1102	40-41	-0.29614304864930574
1102	42-43	-0.39595951211675384
1102	44-45	-0.31133522917707523
1102	46-47	-0.21806651201794125
1102	48-49	-0.2966975794687343
1102	50-51	-0.18237829971661057
1102	52-53	-0.36544060641710274
1102	54-55	-0.3034664835015235
1102	56-57	-0.36512318550404643
1102	58-59	-0.30046539732263255
1102	60-61	-0.31987146567990266
1102	62-63	-0.4257588951506577
1102	64-65	-0.43312491208150306
1102	66-67	-0.42134885595505267
1102	68-69	-0.33653174946106645
1102	70-71	-0.32586589442319536
1102	72-73	-0.4351993655716768
1102	74-75	-0.5156422224450736
1102	76-77	-0.41122459711670345
1102	78-79	-0.45329744260281046
1102	80-81	-0.29644060802376515
1102	82-83	-0.3673968213912282
1102	84-85	-0.4081660838325405
1102	86-87	-0.4344855614370182
1102	88-89	-0.33886899449770524
1102	90-91	-0.5228633366083848
1102	92-93	-0.45731544426209325
1102	94-95	-0.6642476922131628
1102	96-97	-0.7140268339817624
1102	98-99	-0.6743347302499529
1102	100	-0.6568584613534085
1103	1	-0.12082756654303495
1103	2	-0.16320041232423677
1103	3	-0.27659379883470336
1103	4	-0.13019734962016116
1103	5	-0.13087173638390226
1103	6	-0.18081571200467295
1103	7	-0.1502293203358036
1103	8	-0.1594236087412213
1103	9	-0.18192455734661905
1103	10-11	-0.27749483661335717
1103	12-13	-0.18618952291927116
1103	14-15	-0.29313046947201116
1103	16-17	-0.3232853048605051
1103	18-19	-0.3796126393410475
1103	20-21	-0.34047940252985853
1103	22-23	-0.37262015002607995
1103	24-25	-0.30572328603954446
1103	26-27	-0.35046773947071586
1103	28-29	-0.2255137082467158
1103	30-31	-0.4362645740251665
1103	32-33	-0.32803803499072615
1103	34-35	-0.4721090478116352
1103	36-37	-0.5008802916740294
1103	38-39	-0.4472002096704202
1103	40-41	-0.4393974026084919
1103	42-43	-0.4347617122742591
1103	44-45	-0.3117455779922551
1103	46-47	-0.47323923734217743
1103	48-49	-0.505128048210949
1103	50-51	-0.5501149512304053
1103	52-53	-0.4322340662570028
1103	54-55	-1.0866953292911248
1103	56-57	-0.41781979903596067
1103	58-59	-0.43541289809522965
1103	60-61	-0.42093472145444366
1103	62-63	-0.426595730093986
1103	64-65	-0.45999166896758936
1103	66-67	-0.43650148129025723
1103	68-69	-0.4752974381691857
1103	70-71	-0.5749272511039152
1103	72-73	-0.7084751653344057
1103	74-75	-0.6812989051618246
1103	76-77	-0.551264323567441
1103	78-79	-0.4890724946908165
1103	80-81	-1.3137039814324645
1103	82-83	-0.40575994956261496
1103	84-85	-0.3421979945900837
1103	86-87	-0.3620962546810311
1103	88-89	-0.3515932346109558
1103	90-91	-0.43606428166924616
1103	92-93	-0.4729753045964564
1103	94-95	-0.4484933756377103
1103	96-97	-0.5982490902647228
1103	98-99	-0.4125321861620641
1103	100	-0.455994149363697
1104	1	-0.06089537823481095
1104	2	-0.14783809401507852
1104	3	-0.10319002592320459
1104	4	0.01104861076842667
1104	5	-0.06102447158585278
1104	6	-0.21782270829385908
1104	7	-0.1626229214935364
1104	8	-0.07815444961597962
1104	9	-0.10594790031146317
1104	10-11	-0.16126989931594693
1104	12-13	-0.14722536240063988
1104	14-15	-0.25342958405764193
1104	16-17	-0.33453562265142267
1104	18-19	-0.25768421074377557
1104	20-21	-0.28215288336706834
1104	22-23	-0.1994702126064496
1104	24-25	-0.14049113977750238
1104	26-27	-0.28544414349541114
1104	28-29	-0.35793544001163013
1104	30-31	-0.32543621534190237
1104	32-33	-0.26876345997495577
1104	34-35	-0.2925497565831563
1104	36-37	-0.3664130253603517
1104	38-39	-0.19561318018431706
1104	40-41	-0.12293675001262727
1104	42-43	-0.20778071372632922
1104	44-45	-0.30711738113789977
1104	46-47	-0.1987697342693835
1104	48-49	-0.2553769946118436
1104	50-51	-0.11727043532373926
1104	52-53	-0.22138550951665792
1104	54-55	-0.1858496082235419
1104	56-57	-0.22059582488947171
1104	58-59	-0.2800997701526029
1104	60-61	-0.30120678103291
1104	62-63	-0.27456281551129536
1104	64-65	-0.45807040899995144
1104	66-67	-0.28464717131210904
1104	68-69	-0.10936816864826682
1104	70-71	-0.1045519339241352
1104	72-73	-0.2213995689875361
1104	74-75	-0.3231726859447619
1104	76-77	-0.1831650310189019
1104	78-79	-0.07312736618106186
1104	80-81	-0.13949005559019412
1104	82-83	-0.14799506472515134
1104	84-85	-0.8564534838169742
1104	86-87	-0.17324585719793717
1104	88-89	-0.18254475560715733
1104	90-91	-0.2916297783764463
1104	92-93	-0.15980950207661593
1104	94-95	-0.13773160026210363
1104	96-97	-0.06573037115766667
1104	98-99	-0.2417573385794789
1104	100	-1.3781148414634146
1105	1	-0.07107512013110551
1105	2	-0.1860733930635945
1105	3	-0.21286865127334664
1105	4	-0.11451798491062704
1105	5	-0.09951106536806265
1105	6	-0.1291614715573317
1105	7	-0.13757302179588038
1105	8	-0.16894034530537283
1105	9	-0.22094191522173645
1105	10-11	-0.1869222597261242
1105	12-13	-0.10914259452535902
1105	14-15	-0.38152457478422974
1105	16-17	-0.33606877798880674
1105	18-19	-0.30992387730314874
1105	20-21	-0.3105390095506735
1105	22-23	-0.3744801997309608
1105	24-25	-0.14270585050056184
1105	26-27	-0.2971704227574463
1105	28-29	-0.2994637897937835
1105	30-31	-0.2774613742736918
1105	32-33	-0.24656620755984449
1105	34-35	-0.3176312402627133
1105	36-37	-0.32917518139132795
1105	38-39	-0.266444346048182
1105	40-41	-0.2010066950906335
1105	42-43	-0.2540568155672105
1105	44-45	-0.35761774326097395
1105	46-47	-0.3856193437416806
1105	48-49	-0.37085012804940476
1105	50-51	-0.24575024169327975
1105	52-53	-0.37208388374690315
1105	54-55	-1.3255457098440928
1105	56-57	-0.3491828970477684
1105	58-59	-0.35539969523718895
1105	60-61	-0.2974074226038681
1105	62-63	-0.12044912012193976
1105	64-65	-0.17367895539194222
1105	66-67	-0.2858282326105481
1105	68-69	-0.07442760281659133
1105	70-71	-0.15852512699705557
1105	72-73	-0.20703789832602126
1105	74-75	-0.3000712335246689
1105	76-77	-0.29138208141149136
1105	78-79	-0.21868767021116042
1105	80-81	-0.19237942411653108
1105	82-83	-0.20489739519790362
1105	84-85	-0.21057569340959148
1105	86-87	-0.2477051394278469
1105	88-89	-0.37663206654633896
1105	90-91	-0.2473794222408543
1105	92-93	-0.21342410468965056
1105	94-95	-0.34868802071382277
1105	96-97	-0.3094317168568317
1105	98-99	-0.23223939336927302
1105	100	-0.18910419906857356
1106	1	-0.05996395073189831
1106	2	-0.14299831099395277
1106	3	-0.12311488353045519
1106	4	-0.07249465334924565
1106	5	-0.14982535481278347
1106	6	-0.1005867788522039
1106	7	-0.0650945067901958
1106	8	-0.20390731926691075
1106	9	-0.1617325700069543
1106	10-11	-0.18361796552509446
1106	12-13	-0.1926395717044045
1106	14-15	-0.18088183188604745
1106	16-17	-0.15387194606370969
1106	18-19	-0.22499292538849858
1106	20-21	-0.21961825572230964
1106	22-23	-0.12013225706780162
1106	24-25	-0.06565195265861945
1106	26-27	-0.017165194314721077
1106	28-29	-0.061735329578908704
1106	30-31	-0.17750823790751014
1106	32-33	-0.057838164687780136
1106	34-35	-0.06753410314112074
1106	36-37	-0.13060813608515076
1106	38-39	-0.15227549940650675
1106	40-41	-0.18264095940151748
1106	42-43	-0.1470873946711606
1106	44-45	-0.14372512393356374
1106	46-47	-0.287993913575491
1106	48-49	-0.25168319325747035
1106	50-51	-0.1295313442243824
1106	52-53	-0.21322913419921719
1106	54-55	-0.22514381338240952
1106	56-57	-0.16502714774256333
1106	58-59	-0.11664475240616667
1106	60-61	-0.0867513667032469
1106	62-63	-0.24638747112823012
1106	64-65	-0.11945732304531731
1106	66-67	-0.13725527106203828
1106	68-69	-0.04434208251367977
1106	70-71	-0.04526752739433704
1106	72-73	-0.09916419581917069
1106	74-75	-0.05369098293245855
1106	76-77	-0.11163116395677264
1106	78-79	-0.029664252383390988
1106	80-81	0.024172764718272788
1106	82-83	-0.08285875135219811
1106	84-85	0.04418559932724264
1106	86-87	0.0758114283821989
1106	88-89	-0.017298625962897773
1106	90-91	0.02571435282303014
1106	92-93	0.06618651330412106
1106	94-95	-0.05097888883349455
1106	96-97	0.09425599647761729
1106	98-99	-0.04912638906061417
1106	100	0.020324834001762326
1107	1	8.045797406097677E-4
1107	2	-0.11090885147618224
1107	3	-0.1168737713688941
1107	4	-0.06557227672289656
1107	5	-0.09997552327932624
1107	6	-0.16718251223795022
1107	7	-0.1883701108243514
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2308	78-79	0.4984644271895249
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2308	84-85	0.4469136741923201
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2308	90-91	0.3659178859001848
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2308	94-95	0.3133208260816822
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2309	2	0.09540105542184563
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2314	4	-0.12589130572910534
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2314	52-53	0.23054648344876938
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2314	76-77	0.40596827722423967
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2314	80-81	0.21883532029513475
2314	82-83	0.2211287087909568
2314	84-85	0.17093357105833817
2314	86-87	0.1565166145658914
2314	88-89	0.19357342475948158
2314	90-91	0.20420246855847068
2314	92-93	-0.0335130311060432
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2314	96-97	0.05098942316264399
2314	98-99	0.07083789713786004
2314	100	-0.11710861418126584
2315	1	0.09190506405126797
2315	2	0.05669448023721202
2315	3	0.04980490787684033
2315	4	0.002964369159904834
2315	5	0.02160709116085968
2315	6	0.08687812585620236
2315	7	0.019411258550647403
2315	8	0.06801977103682333
2315	9	0.09940634718566344
2315	10-11	0.134647688570098
2315	12-13	0.08029986791196819
2315	14-15	0.06251245977157538
2315	16-17	0.20785209852767395
2315	18-19	0.1988057390309308
2315	20-21	0.1403902185120529
2315	22-23	0.24242788946820326
2315	24-25	0.32529399086153177
2315	26-27	0.256060588401148
2315	28-29	-1.099268207670967
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2315	32-33	-1.3950973097249815
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2315	64-65	0.6109078421640461
2315	66-67	0.4798964779840347
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2315	80-81	0.3705608178251296
2315	82-83	0.3223029335914802
2315	84-85	0.37757867707923154
2315	86-87	0.4024332409690352
2315	88-89	0.3918084300280107
2315	90-91	0.4785468585499828
2315	92-93	0.5031908270235483
2315	94-95	0.47666641682634747
2315	96-97	0.5051596861286924
2315	98-99	0.5282502844362682
2315	100	0.4187741450881006
2316	1	0.0038053395170223325
2316	2	-0.1101726395614051
2316	3	-0.08787366282940923
2316	4	-0.004013321215516896
2316	5	-0.09452191391294207
2316	6	-0.03725189182052446
2316	7	0.015691122584101436
2316	8	0.02557067422200987
2316	9	-0.14421543415951987
2316	10-11	-0.022707570560520196
2316	12-13	-0.03570583151890361
2316	14-15	0.011909549785364959
2316	16-17	-1.3551619221801232
2316	18-19	0.08975081374916272
2316	20-21	-0.023364023700949588
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2316	24-25	0.1359539120106419
2316	26-27	-0.02428935287866807
2316	28-29	0.01057546248205199
2316	30-31	0.05747978416223276
2316	32-33	0.15248078609611326
2316	34-35	0.14654917870337414
2316	36-37	0.09616678361032172
2316	38-39	0.14725238405889485
2316	40-41	-0.09983701264640388
2316	42-43	-0.038351409572214834
2316	44-45	0.04409031296279409
2316	46-47	0.10722467010200631
2316	48-49	0.059494401689754284
2316	50-51	0.09173413404371189
2316	52-53	0.05848063856335983
2316	54-55	0.10303770013656788
2316	56-57	0.01664941923448282
2316	58-59	0.07032933877063385
2316	60-61	-1.409291148607359
2316	62-63	0.0988228924010528
2316	64-65	0.12360758179969622
2316	66-67	-0.11206114910033449
2316	68-69	0.15686691159310584
2316	70-71	0.15387044101860425
2316	72-73	0.22342477147728346
2316	74-75	0.1115931632018956
2316	76-77	-0.13344296592165605
2316	78-79	-0.2897875004078543
2316	80-81	-0.0809442323802898
2316	82-83	-0.12397661021134354
2316	84-85	-0.7145561218091991
2316	86-87	-0.010006151172575528
2316	88-89	0.06527014325508418
2316	90-91	0.08689940010243191
2316	92-93	0.3080994230920595
2316	94-95	0.1716670775126765
2316	96-97	0.1509290856996479
2316	98-99	0.23271214193566436
2316	100	0.03886352651040781
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	2.0
11	6.0
12	49.0
13	293.0
14	1101.0
15	2991.0
16	6658.0
17	12293.0
18	20191.0
19	28063.0
20	37293.0
21	48268.0
22	60365.0
23	70075.0
24	75777.0
25	83881.0
26	96168.0
27	113106.0
28	135764.0
29	165126.0
30	199413.0
31	245522.0
32	306876.0
33	397343.0
34	565115.0
35	999231.0
36	1401704.0
37	1062049.0
38	946614.0
39	168977.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.487915063519665	18.188007402578982	13.057656442401788	23.266421091499563
2	16.638541147675664	20.989320303176267	38.4619437864811	23.91019476266697
3	19.941964653304385	26.196108385058004	29.622246138114416	24.239680823523198
4	13.361352952307708	16.620885259232736	36.640619353756676	33.377142434702876
5	13.673446362904865	36.776475254931675	33.3307771433584	16.21930123880506
6	30.337193484448527	35.18552820552005	18.90860747541483	15.568670834616594
7	27.800122117282523	32.108845751693664	21.884454210639486	18.20657792038433
8	26.809608507548816	33.13305954644208	20.42589879079908	19.631433155210022
9	27.258724300932542	16.299908046566753	20.67046148706921	35.77090616543149
10-11	25.563692415654405	25.277156816554324	27.93447623913027	21.22467452866101
12-13	26.367438227301243	23.320085542966886	27.78525821674595	22.52721801298592
14-15	23.610641927425128	24.726132750422128	26.56165059839944	25.101574723753306
16-17	22.64066613629693	27.176941109098014	27.262641153013504	22.91975160159155
18-19	22.402196954499274	26.658099783591265	28.860178137745635	22.079525124163833
20-21	23.322549483142048	25.571524290971965	28.72625562248874	22.379670603397244
22-23	22.968834597051583	25.41567743465552	28.599262643866062	23.016225324426838
24-25	23.04142749144183	25.56794130389296	28.53491867479144	22.85571252987377
26-27	22.83014885718897	25.161893056228042	29.15731050056351	22.85064758601948
28-29	22.58193714014915	26.153562524687235	28.846044686596333	22.418455648567278
30-31	23.435849065973084	25.415680186888146	28.718879333223352	22.42959141391542
32-33	23.157682973062126	25.61798389781466	28.72589505034252	22.49843807878069
34-35	23.421431555768343	25.719786988487463	28.56900976847612	22.289771687268072
36-37	23.317062351590998	25.548089602744046	28.70036214365374	22.434485902011215
38-39	23.13863513794963	26.043653130780086	28.38090042771765	22.436811303552638
40-41	23.094526224740655	25.926810215345085	28.366529473837605	22.612134086076654
42-43	22.886080604168498	26.069235732695827	27.867461031865552	23.177222631270123
44-45	22.928921576234046	25.823044054190813	28.01974316750968	23.228291202065463
46-47	23.13836002038256	25.929781963418986	27.87672633099813	23.055131685200323
48-49	23.100938569972147	26.01603232592326	27.8774808110698	23.005548293034792
50-51	22.603907218914603	26.371641228242424	28.077291315290303	22.947160237552662
52-53	22.941178985944852	26.63597617850178	27.16745480362603	23.25539003192734
54-55	22.62096975525088	26.1851600208438	27.651253400289484	23.542616823615838
56-57	23.159009708313366	26.137033608999786	27.245862537708827	23.458094144978023
58-59	23.401709206003794	26.0610465396965	27.41375414811298	23.123490106186726
60-61	23.285019636986263	26.333257542545425	27.806060821727996	22.575661998740316
62-63	22.9240625240073	26.402546949485124	27.697696361262324	22.97569416524525
64-65	22.755505418716357	26.524556404254422	27.38417347671249	23.335764700316723
66-67	22.880469417370847	26.175951931696535	27.147153388950425	23.796425261982197
68-69	22.580316369891314	26.255097042503344	27.382909824836695	23.781676762768647
70-71	22.96841124699792	26.12696242702296	27.355212411127276	23.549413914851836
72-73	22.82688888419936	26.213991820631534	27.318941716448098	23.64017757872101
74-75	22.66940835798382	26.928096533722773	26.816821070710645	23.585674037582763
76-77	22.601199441658252	26.166241841424327	27.19029136390972	24.042267353007706
78-79	22.60831059363038	26.34288612346638	27.23152070407196	23.81728257883128
80-81	22.786425226344267	26.427753099844292	26.8769970006501	23.908824673161337
82-83	22.80952425942008	26.326334520333745	27.100097326356103	23.764043893890065
84-85	22.589495850707106	26.78358632195335	26.76084272408995	23.86607510324959
86-87	22.445049469786778	26.707009351917172	26.82785810684536	24.02008307145069
88-89	22.191471400828107	26.431641261953576	26.844880901981387	24.532006435236926
90-91	22.858392841146156	26.13982152793816	26.840240133670264	24.16154549724542
92-93	22.807887069542005	26.4899180711173	26.792523171392478	23.909671687948215
94-95	22.42446218199031	26.248136656585398	26.860384960404986	24.46701620101931
96-97	22.072021716703986	26.212266671668775	27.307018778538154	24.408692833089077
98-99	22.3655228363329	26.28434028800713	26.717055777521054	24.63308109813891
100	22.213364474599402	26.025553621103164	26.82491630462922	24.93616559966821
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3466.0
1	2612.5
2	5161.5
3	7107.0
4	4081.5
5	2790.5
6	3787.5
7	6690.0
8	10106.5
9	11551.5
10	10166.0
11	8177.5
12	8138.5
13	8841.5
14	9609.5
15	10225.5
16	10885.0
17	11754.0
18	12571.0
19	13298.5
20	14348.0
21	15743.5
22	17481.0
23	20163.5
24	23414.5
25	27835.5
26	33963.0
27	40865.0
28	47488.5
29	55341.5
30	65585.0
31	75013.0
32	85241.0
33	96969.0
34	109160.0
35	121120.0
36	137140.5
37	153158.0
38	162415.5
39	171105.5
40	176541.5
41	180401.0
42	188187.0
43	199056.0
44	209882.5
45	223464.0
46	239348.0
47	268619.0
48	321520.0
49	338460.0
50	327482.0
51	314436.0
52	288949.5
53	277667.5
54	271321.5
55	257758.0
56	237566.5
57	217127.0
58	198789.0
59	175413.0
60	146963.5
61	120639.5
62	99223.5
63	78534.0
64	59250.0
65	45257.0
66	34420.5
67	26600.0
68	20896.5
69	16271.5
70	12833.5
71	10070.0
72	8430.0
73	7250.0
74	5801.0
75	4294.5
76	3008.5
77	2011.5
78	1315.0
79	868.5
80	560.5
81	345.5
82	240.5
83	167.0
84	108.0
85	68.0
86	45.5
87	33.0
88	23.0
89	16.5
90	11.5
91	8.0
92	5.5
93	2.5
94	2.0
95	2.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04420440281368954
2	0.01055112890872777
3	0.0
4	1.7930022982151766E-4
5	4.827313879810091E-4
6	2.758465074177195E-5
7	2.758465074177195E-5
8	1.3792325370885975E-5
9	0.0
10-11	1.3792325370885975E-5
12-13	2.4136569399050455E-4
14-15	0.011309706804126499
16-17	0.0016688713698772028
18-19	1.1723476565253077E-4
20-21	8.413318476240443E-4
22-23	6.275508043753118E-4
24-25	0.004413544118683512
26-27	0.016261151612274562
28-29	0.013302697820219523
30-31	0.009999435893892331
32-33	0.007220282331658807
34-35	0.009102934744784743
36-37	0.005316941430476543
38-39	0.004123905285894906
40-41	0.005441072358814517
42-43	0.007047878264522732
44-45	0.007013397451095517
46-47	0.014826749773702422
48-49	0.01713696427332582
50-51	0.017068002646471393
52-53	0.011351083780239156
54-55	0.004013566682927818
56-57	0.0038963319172752877
58-59	0.010027020544634103
60-61	0.00258606100704112
62-63	1.3792325370885975E-4
64-65	4.068735984411362E-4
66-67	8.206433595677154E-4
68-69	4.8273138798100905E-5
70-71	1.6550790445063167E-4
72-73	0.0029791422801113705
74-75	0.0052824606170493275
76-77	0.0050893680618569245
78-79	0.0033239504143835197
80-81	6.206546416898688E-5
82-83	8.344356849386014E-4
84-85	2.3446953130506153E-4
86-87	3.3101580890126334E-4
88-89	2.1378104324873258E-4
90-91	4.2066592381202213E-4
92-93	4.896275506664521E-4
94-95	1.3792325370885975E-5
96-97	0.0018964447384968214
98-99	4.5514673723923713E-4
100	1.103386029670878E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7250409.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.5279159686836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.6277175746949	36.392990962487964
2	16.813594637549727	17.32738983230343
3	5.784298609714353	8.941585580974037
4	2.450004562990695	5.049745169787036
5	1.2386032710155273	3.191132263371236
6	0.7224906071128208	2.2337061174883575
7	0.4711566911138959	1.699440567145989
8	0.3217693163367212	1.326408183479947
9	0.23901926656903455	1.1084548214399037
>10	1.2086378935684232	11.120764522753895
>50	0.07779933110259024	2.755721337679696
>100	0.0403992145154444	3.7551172873137904
>500	0.002849707002110061	1.0038792555604656
>1k	0.0012625235864255082	1.2452868157924257
>5k	2.164326147969757E-4	0.7238414326782935
>10k+	1.8036051233081307E-4	2.124535849743513
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	31282	0.431451522252055	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28919	0.3988602574006515	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26278	0.36243472609614164	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15939	0.21983587408655153	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11883	0.16389420238223804	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	9506	0.13110984497564207	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	5.516930148354389E-5
10-11	0.0	0.0	0.0	0.0	5.516930148354389E-5
12-13	0.0	2.068848805632896E-5	0.0	0.0	5.516930148354389E-5
14-15	0.0	2.7584650741771946E-5	0.0	2.068848805632896E-5	8.965011491075882E-5
16-17	0.0	2.7584650741771946E-5	0.0	4.137697611265792E-5	1.1723476565253077E-4
18-19	0.0	2.7584650741771946E-5	0.0	5.516930148354389E-5	1.3102709102341675E-4
20-21	0.0	4.137697611265792E-5	0.0	1.3792325370885972E-4	1.517155790797457E-4
22-23	0.0	4.137697611265792E-5	0.0	3.654966223284783E-4	1.9998871787784661E-4
24-25	0.0	4.137697611265792E-5	0.0	8.827088237367023E-4	2.2067720593417557E-4
26-27	0.0	4.137697611265792E-5	0.0	0.002020575666834795	2.2067720593417557E-4
28-29	0.0	4.137697611265792E-5	0.0	0.00542728003344363	2.2067720593417557E-4
30-31	0.0	4.137697611265792E-5	0.0	0.014006106414134707	2.2757336861961856E-4
32-33	0.0	4.137697611265792E-5	0.0	0.03553592631808771	2.3446953130506156E-4
34-35	0.0	8.965011491075882E-5	0.0	0.06757549815465583	2.620541820468335E-4
36-37	0.0	1.2413092833797376E-4	0.0	0.1167382419391789	2.7584650741771944E-4
38-39	0.0	1.3792325370885972E-4	0.0	0.19113404498973782	2.7584650741771944E-4
40-41	0.0	1.517155790797457E-4	0.0	0.2826185391748245	2.9653499547404845E-4
42-43	0.0	1.517155790797457E-4	0.0	0.368013721708665	3.172234835303774E-4
44-45	0.0	1.517155790797457E-4	0.0	0.4724630017423845	3.241196462158204E-4
46-47	0.0	1.5861174176518868E-4	0.0	0.6005385351364316	3.4480813427214933E-4
48-49	0.0	1.6550790445063167E-4	0.0	0.7307449828002807	3.8618511038480724E-4
50-51	0.0	1.7930022982151766E-4	0.0	0.8699302342805765	4.068735984411362E-4
52-53	0.0	1.7930022982151766E-4	0.0	1.016832291805883	4.6893906261012306E-4
54-55	0.0	2.1378104324873258E-4	0.0	1.186443413054353	5.24108364093667E-4
56-57	0.0	2.3446953130506156E-4	0.0	1.381591024727019	5.37900689464553E-4
58-59	0.0	2.482618566759475E-4	0.0	1.595103117631019	5.585891775208819E-4
60-61	0.0	2.482618566759475E-4	0.0	1.8128218697731397	5.792776655772109E-4
62-63	0.0	2.482618566759475E-4	0.0	2.048160317576567	5.930699909480969E-4
64-65	0.0	2.620541820468335E-4	0.0	2.285774499066191	5.930699909480969E-4
66-67	0.0	2.620541820468335E-4	0.0	2.5491734328366853	5.930699909480969E-4
68-69	0.0	2.620541820468335E-4	0.0	2.865576273007495	5.930699909480969E-4
70-71	1.3792325370885973E-5	2.689503447322765E-4	0.0	3.1997712129067475	6.758239431734127E-4
72-73	1.3792325370885973E-5	2.7584650741771944E-4	0.0	3.5136086805585727	7.861625461405005E-4
74-75	1.3792325370885973E-5	2.7584650741771944E-4	0.0	3.834073360551108	7.861625461405005E-4
76-77	1.3792325370885973E-5	2.7584650741771944E-4	0.0	4.182129587448101	7.930587088259435E-4
78-79	1.3792325370885973E-5	2.7584650741771944E-4	0.0	4.585162023273446	7.999548715113865E-4
80-81	1.3792325370885973E-5	2.7584650741771944E-4	0.0	4.991842529159389	8.206433595677155E-4
82-83	1.3792325370885973E-5	2.7584650741771944E-4	0.0	5.410667177534398	8.827088237367024E-4
84-85	1.3792325370885973E-5	2.7584650741771944E-4	0.0	5.839829173774886	8.965011491075883E-4
86-87	1.3792325370885973E-5	2.7584650741771944E-4	0.0	6.285590233599235	9.102934744784743E-4
88	1.3792325370885973E-5	2.7584650741771944E-4	0.0	6.634011957118557	9.102934744784743E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	12070	0.0	53.537685	2
GTACCTG	14410	0.0	45.352757	1
ACCTGGG	14020	0.0	45.1831	3
CCTGGGG	12335	0.0	33.79237	4
TATAACG	740	0.0	26.038519	2
CTGGGGG	8105	0.0	22.554373	5
TAACGCA	945	0.0	22.377674	4
CATGGGG	19740	0.0	20.925505	4
TATCACG	640	0.0	20.560904	2
TGGGGGG	12470	0.0	20.349583	6
ATGGGGG	11510	0.0	20.16907	5
GAGTACT	27855	0.0	19.852596	12-13
GTACATG	40720	0.0	19.836647	1
ATAACGC	1070	0.0	19.763458	3
GTACACG	1730	0.0	19.567677	1
TACATGG	41905	0.0	19.065458	2
TACCGGG	975	0.0	18.798542	2
ACATGGG	41495	0.0	18.57301	3
AGTACTT	29160	0.0	17.972807	12-13
GGGTTAG	1935	0.0	17.494616	1
>>END_MODULE