FastQCFastQC Report
Mon 6 Jun 2016
ERR523019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703212
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126630.34194639680363964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101230.27335729091394173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52100.14068867782886857No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80450.042.0890051
GTATCAA132300.036.5422671
TCAACGC157050.030.366284
ATCAACG159150.029.9951443
CAACGCA159200.029.9836965
TATCAAC161950.029.5628742
AACGCAG164550.029.024476
ACGCAGA186350.025.5768877
CGCAGAG189350.025.0961638
GTACATG152400.024.1623421
TACATGG154000.023.2709482
GCAGAGT208350.022.5136999
ACATGGG153150.022.448743
GAGTACT103350.021.78415312-13
CATGGGG90300.021.4064984
TACCTGG34200.020.9024562
GTACTTT113850.019.59048814-15
CAGAGTA203150.019.38621310-11
AGAGTAC184500.019.23164210-11
AGTACTT111350.018.97383512-13
Produced by FastQC (version 0.11.5)