FastQCFastQC Report
Mon 6 Jun 2016
ERR523019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703212
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116270.3139706827478416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100410.2711429969442743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51030.13779929423430254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83450.042.323621
GTATCAA135900.034.9865341
TCAACGC158450.029.7510434
ATCAACG161000.029.3090213
CAACGCA160800.029.2870185
TATCAAC164900.028.6458912
AACGCAG167900.028.2433626
ACGCAGA190700.024.7187297
CGCAGAG195600.024.1472268
GTACATG149000.023.8618831
TACATGG151350.022.9498042
TACCTGG35450.022.804952
TATAACG3750.022.5609912
ACATGGG150250.022.0843033
GCAGAGT214300.021.8646769
GAGTACT108100.021.5636912-13
ATAACGC4100.020.6339383
GTACTTT112600.020.18005214-15
CATGGGG86900.020.0653924
CAGAGTA210200.019.10510410-11
Produced by FastQC (version 0.11.5)