FastQCFastQC Report
Mon 6 Jun 2016
ERR523018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8108737
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223500.2756286213253679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169590.20914477803386644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102160.12598756131811895No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160950.036.573821
GTATCAA260750.034.973041
TCAACGC302350.029.577444
CAACGCA305650.029.3038675
ATCAACG305800.029.2462833
AACGCAG318400.028.286656
TATCAAC322950.027.7847922
GTACATG298800.027.259661
TACATGG309400.025.8416672
ACATGGG307450.025.1608163
ACGCAGA364950.024.6787937
CATGGGG183750.024.2956244
CGCAGAG376500.023.90828
TACCTGG60050.023.3259722
GCAGAGT417650.021.2255869
GAGTACT213850.020.25689712-13
ATGGGGG100450.019.74195
AGAGTAC344350.019.54346310-11
GTATAGG28150.018.3873141
GTACTTT233950.018.35567714-15
Produced by FastQC (version 0.11.5)