FastQCFastQC Report
Mon 6 Jun 2016
ERR523018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8108737
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201160.2480780915696242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161090.19866225775974727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90300.1113613624415245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169350.036.4152951
GTATCAA261600.032.4388241
TCAACGC306550.027.1185684
GTACATG291500.026.9975091
ATCAACG310350.026.7720433
CAACGCA311650.026.674795
AACGCAG324050.025.6967726
TATCAAC328300.025.5115032
TACATGG301100.025.5045952
ACATGGG297600.024.6660213
TACCTGG59750.023.296832
ACGCAGA367750.022.6301487
CATGGGG177250.022.456264
CGCAGAG379450.021.912878
GCAGAGT421550.019.445579
GAGTACT215400.019.31195412-13
AGAGTAC343450.019.29681210-11
ATGGGGG97700.018.4223275
GTACTTT230900.017.80997314-15
AGTACTT236050.017.6125612-13
Produced by FastQC (version 0.11.5)