FastQCFastQC Report
Mon 6 Jun 2016
ERR523017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6816782
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT619210.9083611592684054No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257470.37770021103799417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197540.28978482809043915No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT174980.2566900335084795No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC142520.20907225726156417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124450.18256414830340767No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT124370.1824467908758121No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC115560.16952280416184645No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA98420.14437897529948882No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG93180.1366920637919769No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA89780.13170437311916383No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC82400.1208781504234696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG57900.031.4204482
GTACATG250850.029.8362431
TACATGG256950.028.9792082
CATGGGG186450.027.8332754
ACATGGG259950.027.7056333
ATGGGGG129200.027.397195
TATAACG5950.026.862222
GTACCTG70150.026.5388871
ATAACGC6950.026.3802533
TAACGCA6950.025.7013764
ACCTGGG76750.023.5820473
TGGGGGG140500.022.8051156
GAGTACT218250.020.08502612-13
AGAGTAC327850.019.33079710-11
AGTACTT230250.018.58932912-13
GTATAAC20350.018.250631
GTACTTT249150.017.53747414-15
CCTGGGG74050.017.5203024
TATCACG5950.017.3814372
GAGTACA188150.016.5912481
Produced by FastQC (version 0.11.5)