FastQCFastQC Report
Mon 6 Jun 2016
ERR523017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6816782
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT622880.9137449312593536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231420.33948569867717643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188320.27625938456004606No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT172240.25267054161333014No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC143470.21046587671426195No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC120490.17675495563742538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117160.1718699527137585No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA102440.1502761860361678No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA94390.1384670948843604No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG57150.033.0613252
GTACATG238500.028.0575661
GTACCTG72450.026.6120871
TACATGG245800.026.5601652
ACATGGG249700.025.2198493
ATGGGGG122800.024.7987445
CATGGGG178200.024.6053874
ACCTGGG79450.023.5421123
TGGGGGG123000.021.6636186
GAGTACT220450.020.69532812-13
GGGTTAG22850.019.7568871
AGAGTAC334600.019.74635910-11
TATAACG7700.018.9226252
AGTACTT231800.018.82007812-13
GTACTTT245750.018.38038414-15
TAACGCA7400.017.782084
ATAACGC7500.017.5449873
CCTGGGG72600.016.7656124
GTACACG13200.016.3876761
GTTAGAG27750.016.25793
Produced by FastQC (version 0.11.5)