FastQCFastQC Report
Mon 6 Jun 2016
ERR523016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7865789
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178260.22662697918797467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149190.18966946608916155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81750.1039310868877871No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131050.043.9851571
GTATCAA216250.035.751031
TCAACGC254800.029.7871514
ATCAACG260350.029.136163
CAACGCA261800.029.1146935
TATCAAC265350.028.714242
GTACATG271450.028.2382851
AACGCAG271850.028.2045636
TACATGG282050.026.7307822
TACCTGG68950.026.4503172
ACATGGG284600.025.8608763
ACGCAGA308550.024.697557
CATGGGG206900.023.7589444
CGCAGAG320600.023.7537128
GTACCTG86600.021.5714681
GCAGAGT357000.021.067639
ATGGGGG122350.021.0478425
GAGTACT182150.020.64750912-13
ACCTGGG87650.020.536823
AGAGTAC298850.019.06741510-11
Produced by FastQC (version 0.11.5)