FastQCFastQC Report
Mon 6 Jun 2016
ERR523016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7865789
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164650.20932420129754306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141180.1794861265665784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128500.042.472241
GTATCAA211500.036.6159061
TCAACGC253450.029.9993424
CAACGCA259550.029.2580785
ATCAACG260250.029.1438273
TATCAAC267750.028.5964552
TACCTGG70350.028.5463832
AACGCAG270900.028.1708376
GTACATG274600.026.128811
TACATGG286800.024.450392
ACGCAGA312150.024.38797
GTACCTG90450.023.4594761
ACATGGG292200.023.2713783
CGCAGAG327250.023.2377138
ACCTGGG86400.022.5719013
CATGGGG205100.021.4912994
GCAGAGT365050.020.6511279
ATGGGGG118150.020.0855225
GAGTACT181100.019.9577112-13
AGAGTAC309450.019.34231810-11
Produced by FastQC (version 0.11.5)