FastQCFastQC Report
Mon 6 Jun 2016
ERR523015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6004248
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136400.22717249520672697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111720.1860682636693221No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62820.10462592484520958No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81050.049.8238871
GTATCAA140500.039.863371
TCAACGC165800.033.30964
ATCAACG169000.032.6251683
CAACGCA170000.032.4834025
AACGCAG176450.031.474046
TATCAAC177000.031.3929922
ACGCAGA203950.027.09157
GTACATG172700.026.0536691
CGCAGAG212050.025.965858
TACATGG178250.024.4741122
ACATGGG183450.023.2397253
GCAGAGT243900.022.2853939
CATGGGG120600.020.5390014
GAGTACT144650.019.8786112-13
CAGAGTA237750.019.4139710-11
AGAGTAC215950.019.37238310-11
GTGGTAT43750.018.180761
GTACTTT161650.017.74594914-15
GTATACG3751.9645086E-1017.5711481
Produced by FastQC (version 0.11.5)