FastQCFastQC Report
Mon 6 Jun 2016
ERR523015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6004248
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119390.1988425528059467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101600.16921353015398433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81800.047.9126131
GTATCAA141650.038.795771
TCAACGC167150.032.360354
CAACGCA171000.031.686475
ATCAACG170600.031.6794433
TATCAAC178550.030.505642
AACGCAG180400.030.060956
ACGCAGA205600.026.3988537
CGCAGAG211100.025.541698
GTACATG173250.024.8488311
TACATGG175350.024.222192
ACATGGG178200.023.049553
GCAGAGT247400.021.5090489
CATGGGG121150.020.75274
AGAGTAC216400.019.58349410-11
GAGTACT144350.019.00228312-13
CAGAGTA237600.018.91442710-11
TGGTATC45250.018.0868152
ATGGGGG71150.018.0313575
GTGGTAT47450.017.84821
Produced by FastQC (version 0.11.5)