FastQCFastQC Report
Mon 6 Jun 2016
ERR523014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7739107
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224980.29070537466402774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176400.2279332744721064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106510.13762569764185972No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132950.044.5571
GTATCAA228700.038.1230661
TCAACGC268300.031.8782564
ATCAACG271800.031.4712223
CAACGCA273900.031.1560485
AACGCAG284100.030.2143126
TATCAAC286000.029.9780862
GTACATG273500.027.9903771
TACATGG279900.026.7856942
ACGCAGA324750.026.316557
ACATGGG280300.025.9058023
CGCAGAG337000.025.3725668
CATGGGG181700.022.8116914
GCAGAGT375750.022.5176779
GAGTACT208450.020.56685812-13
ATGGGGG99250.020.3590015
AGAGTAC323200.019.75293710-11
CAGAGTA363150.019.36508210-11
GTACTTT229950.018.4201514-15
TACCTGG56600.018.270542
Produced by FastQC (version 0.11.5)