FastQCFastQC Report
Mon 6 Jun 2016
ERR523014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7739107
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207290.2678474402796085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172140.2224287634219297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99560.12864533336985778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136000.043.7921141
GTATCAA231150.037.142411
TCAACGC277000.030.3339144
ATCAACG280700.030.0018123
CAACGCA282300.029.7477665
TATCAAC293650.028.8436372
AACGCAG293950.028.7604456
GTACATG278200.025.3819331
ACGCAGA338750.024.8179637
TACATGG282250.024.5934092
CGCAGAG347200.024.1813168
ACATGGG283850.023.29473
GCAGAGT390350.021.2671419
CATGGGG182650.020.4287384
GAGTACT209450.020.33487312-13
AGAGTAC335050.019.42571310-11
GTACTTT223500.018.9902614-15
TACCTGG58850.018.779662
CAGAGTA379900.018.36980610-11
AGTACTT230150.017.88293512-13
Produced by FastQC (version 0.11.5)