FastQCFastQC Report
Mon 6 Jun 2016
ERR523013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5286731
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202490.38301551563716785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170130.3218056678124913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96450.1824378808000634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90900.053.938791
GTATCAA164900.041.7180561
TCAACGC202000.033.4817474
ATCAACG203400.033.2300763
CAACGCA205650.032.840855
TATCAAC207650.032.7583352
TACCTGG47700.032.3251572
AACGCAG213600.031.8283126
ACGCAGA240100.028.2175567
GTACATG174200.027.2816471
CGCAGAG249600.027.0667388
TACATGG176550.026.360442
GTACCTG61850.026.0946671
ACCTGGG59900.025.266933
ACATGGG183200.024.7072373
GTGGTAT44050.024.3548971
GCAGAGT273350.024.025519
CATGGGG125700.022.920494
TGGTATC44100.022.918442
TATAACG4600.022.4827822
Produced by FastQC (version 0.11.5)