FastQCFastQC Report
Mon 6 Jun 2016
ERR523013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5286731
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185550.35097303040385447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161680.3058222557569129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90810.17176966257598505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91600.054.5936471
GTATCAA164450.040.7647931
TACCTGG52800.035.6286052
TCAACGC204850.032.2305644
ATCAACG206650.031.9043433
CAACGCA208100.031.8175285
TATCAAC211700.031.2791182
AACGCAG214650.030.9114276
GTACCTG64850.029.4523751
ACCTGGG66200.027.968283
ACGCAGA246150.026.8981427
TATCACG5950.026.8741462
GTACATG175250.026.1191561
CGCAGAG254150.025.948138
GTGGTAT42550.025.0975061
TACATGG179700.024.7580152
ACATGGG185550.023.4772013
GCAGAGT281350.023.1889449
CATGGGG129300.022.278684
TGGTATC44650.022.1192572
Produced by FastQC (version 0.11.5)