##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523013_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5286731
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66133060297564	34.0	31.0	34.0	30.0	35.0
2	32.718970002445744	34.0	31.0	34.0	31.0	34.0
3	32.986761384303456	34.0	31.0	34.0	31.0	36.0
4	35.7769515793408	37.0	35.0	37.0	33.0	37.0
5	35.83438801785073	37.0	35.0	37.0	35.0	37.0
6	35.91278580279572	37.0	35.0	37.0	35.0	37.0
7	35.74036148236027	37.0	35.0	37.0	35.0	37.0
8	35.79080815725256	37.0	36.0	37.0	34.0	37.0
9	37.00711082141308	39.0	36.0	39.0	34.0	39.0
10-11	37.1281224635791	38.5	36.0	39.0	34.0	39.0
12-13	36.99335657516904	38.0	36.5	39.0	33.5	39.0
14-15	38.079344967617985	39.0	36.5	41.0	34.5	41.0
16-17	38.099837215095675	39.0	37.0	41.0	34.5	41.0
18-19	38.11236007657662	39.0	37.0	41.0	34.0	41.0
20-21	38.00403311611656	39.0	36.5	41.0	34.0	41.0
22-23	37.912969470169756	39.0	36.5	41.0	34.0	41.0
24-25	37.77811543655238	39.0	36.0	41.0	34.0	41.0
26-27	37.64710905472588	39.0	36.5	41.0	34.0	41.0
28-29	37.60105299475234	39.0	36.5	41.0	34.5	41.0
30-31	37.43739864956246	38.0	36.0	41.0	34.0	41.0
32-33	37.27014359156915	38.0	36.5	41.0	33.0	41.0
34-35	37.07630102609723	38.0	36.0	41.0	32.0	41.0
36-37	37.085256749397686	38.0	35.5	40.5	33.0	41.0
38-39	37.211065306708434	38.0	36.0	41.0	33.0	41.0
40-41	37.10583799327032	38.0	36.5	41.0	32.5	41.0
42-43	37.12060657143327	38.0	36.5	41.0	32.5	41.0
44-45	36.94397766786318	38.0	35.5	41.0	32.5	41.0
46-47	36.66579489291208	38.0	36.0	41.0	31.5	41.0
48-49	36.61105662459467	38.0	36.0	40.5	31.0	41.0
50-51	36.21232024099581	38.0	35.0	40.0	30.0	41.0
52-53	36.263030689475215	38.0	35.5	40.0	30.5	41.0
54-55	36.06695006800989	37.5	35.5	40.0	30.0	41.0
56-57	35.77183300985051	37.0	35.0	40.0	28.5	41.0
58-59	35.64735788524138	36.5	35.0	40.0	29.0	41.0
60-61	35.38423696987799	36.5	35.0	40.0	28.5	41.0
62-63	35.2034156835292	37.5	34.5	39.5	27.5	41.0
64-65	35.06312795184775	37.0	34.5	39.0	28.0	41.0
66-67	34.844974900368484	36.5	34.0	39.0	28.0	41.0
68-69	34.38138899066361	36.0	34.0	38.5	26.5	40.0
70-71	34.13525617248163	36.0	34.0	38.0	26.0	40.0
72-73	33.86921852085911	36.0	34.0	37.0	26.0	39.5
74-75	33.59102581538573	35.5	33.0	37.5	25.5	39.0
76-77	32.108599907958244	34.5	31.0	36.5	23.5	37.5
78-79	33.11057579816337	35.0	33.0	37.0	26.0	38.0
80-81	33.103292658544575	35.0	33.0	36.5	25.5	37.0
82-83	32.9154269434174	35.0	33.0	36.0	25.5	37.5
84-85	32.70693572266113	35.0	33.0	36.0	25.0	37.0
86-87	32.45613035730398	35.0	33.0	36.0	25.0	37.0
88-89	32.28503710516007	35.0	32.5	36.0	24.0	36.5
90-91	32.13269911028195	35.0	32.0	35.5	23.5	37.0
92-93	31.971992900716906	35.0	32.0	35.5	23.0	36.5
94-95	31.84844471943059	35.0	32.0	35.0	23.0	36.0
96-97	31.664472903955204	35.0	31.5	35.0	22.5	36.5
98-99	31.525913556033018	35.0	31.0	35.0	22.0	36.5
100	30.348361397619815	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.06623949745083024
1101	2	-0.05350547939967498
1101	3	-0.11830679469986904
1101	4	-0.14633449513627994
1101	5	-0.10013680682111925
1101	6	-0.14959357378112514
1101	7	-0.09865320635903174
1101	8	-0.07158987760752922
1101	9	-0.10985638513469098
1101	10-11	-0.10254808827748718
1101	12-13	-0.10232647121183192
1101	14-15	-0.3038213746964331
1101	16-17	-0.18869373869066663
1101	18-19	-0.12227225155186972
1101	20-21	-0.14637505254151506
1101	22-23	-0.1521923029597474
1101	24-25	-0.18733447148256488
1101	26-27	-0.16992493121585994
1101	28-29	-0.2554911485690141
1101	30-31	-0.250480347661572
1101	32-33	-0.13154051612883677
1101	34-35	-0.14572422463523083
1101	36-37	-0.3285824721961035
1101	38-39	-0.19728409347737852
1101	40-41	-0.25482941689939054
1101	42-43	-0.22870497005886392
1101	44-45	-0.2805403238452726
1101	46-47	-0.2801464258109405
1101	48-49	-0.3009469507420732
1101	50-51	-0.21351436136730229
1101	52-53	-0.3717405884492635
1101	54-55	-0.27835836460870667
1101	56-57	-0.4122302908799824
1101	58-59	-0.5627483767598349
1101	60-61	-0.5246370482319875
1101	62-63	-0.32344517914697235
1101	64-65	-0.3642626404149496
1101	66-67	-0.3506696239281979
1101	68-69	-0.36335274938641504
1101	70-71	-0.33955453135583014
1101	72-73	-0.587332612240111
1101	74-75	-0.4764108425437499
1101	76-77	-0.393293260736975
1101	78-79	-0.3498328042734329
1101	80-81	-0.2953835924238106
1101	82-83	-0.3834115411960042
1101	84-85	-0.34565026484519024
1101	86-87	-0.5570319865951561
1101	88-89	-0.44818759649388795
1101	90-91	-0.47757887232538465
1101	92-93	-0.7914440445295803
1101	94-95	-0.777835221216165
1101	96-97	-0.5765732807511519
1101	98-99	-0.46856198173168906
1101	100	-0.7318235157130992
1102	1	-0.11262847983521596
1102	2	-0.08976063918287736
1102	3	-0.1258339963573576
1102	4	-0.10551973985954533
1102	5	-0.08999295034565336
1102	6	-0.14238616770433765
1102	7	-0.09785705552499024
1102	8	-0.13220318470339265
1102	9	-0.12906223560958097
1102	10-11	-0.1043883086291757
1102	12-13	-0.07269290712931564
1102	14-15	-0.12683609165704013
1102	16-17	-0.06844966333229507
1102	18-19	-0.10377923561639335
1102	20-21	-0.1686942150901629
1102	22-23	-0.07414180118209401
1102	24-25	-0.11757483899253884
1102	26-27	-0.14359175044687333
1102	28-29	-0.12762287092805025
1102	30-31	-0.10860460583322862
1102	32-33	-0.14762567553293593
1102	34-35	-0.1733213807463443
1102	36-37	-0.16525770383511684
1102	38-39	-0.20725954093839505
1102	40-41	-0.3043940619303953
1102	42-43	-0.32161985621713995
1102	44-45	-0.34456286422155813
1102	46-47	-0.22193101945679672
1102	48-49	-0.30328250091091746
1102	50-51	-0.1680954633186147
1102	52-53	-0.21360501191124115
1102	54-55	-0.2742158386171667
1102	56-57	-0.12881468823670872
1102	58-59	-0.2843370029846284
1102	60-61	-0.24234086676925415
1102	62-63	-0.26649607115254526
1102	64-65	-0.367828218635303
1102	66-67	-0.3971430353130998
1102	68-69	-0.4225163763813171
1102	70-71	-0.3590424039741933
1102	72-73	-0.4133215335154645
1102	74-75	-0.8812699979874807
1102	76-77	-0.5217384861491325
1102	78-79	-0.3667441575709418
1102	80-81	-0.32051663203142056
1102	82-83	-0.3386378564016965
1102	84-85	-0.33809077519536146
1102	86-87	-0.3943560624236042
1102	88-89	-0.3379256838742535
1102	90-91	-0.354910815960622
1102	92-93	-0.5165972338746059
1102	94-95	-0.6096162452233997
1102	96-97	-0.6388469793078784
1102	98-99	-0.8176804863549059
1102	100	-0.5763695604008525
1103	1	-0.09234832450412966
1103	2	-0.05822400596795774
1103	3	-0.13729926642503187
1103	4	-0.009465892624561434
1103	5	-0.14067743831549961
1103	6	-0.06962339878051438
1103	7	-0.062457747399307095
1103	8	-0.07279956479084859
1103	9	-0.06437353184031736
1103	10-11	-0.09223538222585859
1103	12-13	-0.05379069827110783
1103	14-15	-0.13075934400480804
1103	16-17	-0.15285440114728743
1103	18-19	-0.10318938111383602
1103	20-21	-0.10421207940559896
1103	22-23	-0.05108538483947456
1103	24-25	-0.12230103014785243
1103	26-27	-0.16794122681199042
1103	28-29	-0.15521804929962713
1103	30-31	-0.1555215283948641
1103	32-33	-0.07843502442830186
1103	34-35	-0.018169590662594715
1103	36-37	-0.0751907217729908
1103	38-39	-0.14724790304701685
1103	40-41	-0.22829370058842358
1103	42-43	-0.2044512506953069
1103	44-45	-0.2887923492676947
1103	46-47	-0.22364123463487573
1103	48-49	-0.1549387173031178
1103	50-51	-0.10399624336870517
1103	52-53	-0.14084175524637743
1103	54-55	-0.11926353461824846
1103	56-57	0.036741066924157906
1103	58-59	-0.07783544615425342
1103	60-61	-0.15146680985557026
1103	62-63	-0.08299858629651169
1103	64-65	-0.18761059716808148
1103	66-67	-0.1521744743200628
1103	68-69	-0.10194721302419651
1103	70-71	-0.3222204137675675
1103	72-73	-0.24635884055437884
1103	74-75	-0.20179444524617196
1103	76-77	-0.18175129852092908
1103	78-79	-0.27476421613577173
1103	80-81	-0.14917369287895355
1103	82-83	-0.22209457391074494
1103	84-85	-0.2555065405621093
1103	86-87	-0.21907293655449678
1103	88-89	-0.14243888046893005
1103	90-91	-0.24692955426898067
1103	92-93	-0.388181788231865
1103	94-95	-0.08547323939477636
1103	96-97	-0.7102182620675919
1103	98-99	-1.1210633918665103
1103	100	-0.4282048362831823
1104	1	-0.03840915572063608
1104	2	-0.05099825058751861
1104	3	-0.06988307661652016
1104	4	-0.09493587040353901
1104	5	-0.11321336602279075
1104	6	-0.06779822990466755
1104	7	-0.12035802532052031
1104	8	-0.05807417684344074
1104	9	-0.014497780036116126
1104	10-11	-0.0984482824298496
1104	12-13	-0.03830565951032128
1104	14-15	-0.11237471541094379
1104	16-17	-0.035203438985796254
1104	18-19	-0.04391737836390064
1104	20-21	-0.14379295375437096
1104	22-23	-0.09954825478870077
1104	24-25	-0.10930616123316383
1104	26-27	-0.01281975360321752
1104	28-29	-0.17250452845825492
1104	30-31	-0.2628932686150307
1104	32-33	-0.18448279856880845
1104	34-35	-0.11369365277281673
1104	36-37	-0.12879946489852045
1104	38-39	-0.0953434229594734
1104	40-41	-0.09043799599069757
1104	42-43	-0.14279938345647736
1104	44-45	-0.28086677354465195
1104	46-47	-0.11503278424859076
1104	48-49	-0.16170891608514637
1104	50-51	-0.1006223103202899
1104	52-53	-0.10291614029023322
1104	54-55	-0.2578823229252123
1104	56-57	-0.1962598363424135
1104	58-59	-0.2600837379301737
1104	60-61	-0.298507962958503
1104	62-63	-0.09307507876119558
1104	64-65	-0.22353958707581256
1104	66-67	-0.2640441003290732
1104	68-69	-0.05495520691832212
1104	70-71	-0.16189972546214193
1104	72-73	-0.18301912027427392
1104	74-75	-0.20404766982938582
1104	76-77	-0.17729094824181502
1104	78-79	-0.1634647903573878
1104	80-81	-0.034483245021085907
1104	82-83	-0.008373530575980226
1104	84-85	-0.051568873199002496
1104	86-87	-0.05214550812396368
1104	88-89	-0.1867461733883573
1104	90-91	-0.18507224141612966
1104	92-93	-0.20507625331727297
1104	94-95	-0.20844138000860823
1104	96-97	0.09037028718005047
1104	98-99	-0.8508055338547038
1104	100	-0.28520073099241827
1105	1	-0.05954470676010004
1105	2	-1.0143195738185504
1105	3	-0.14472870266450855
1105	4	-0.038993940192924015
1105	5	-0.11680665400529477
1105	6	-0.10474623351301204
1105	7	-0.08169788833509273
1105	8	-0.07971236306408969
1105	9	-0.13955492618722332
1105	10-11	-0.08339382364246006
1105	12-13	-0.08229433833559341
1105	14-15	-0.08400701172154612
1105	16-17	-0.6673852174050197
1105	18-19	-0.09897061857465417
1105	20-21	-0.18435400754341913
1105	22-23	-0.18090582960421386
1105	24-25	-0.147187674990235
1105	26-27	-0.14858505884448903
1105	28-29	-0.20674959477288013
1105	30-31	-0.1838057079426818
1105	32-33	-0.13503698522463026
1105	34-35	-0.13901314348897387
1105	36-37	-0.18172401559359486
1105	38-39	-0.19576768100425568
1105	40-41	-0.20562535417708716
1105	42-43	-0.21750971871340852
1105	44-45	-0.3543363712017964
1105	46-47	-0.16446990665406958
1105	48-49	-0.22994909657914775
1105	50-51	-0.1188127402645307
1105	52-53	-0.19024606913747277
1105	54-55	-0.16009001694866498
1105	56-57	-0.051178435276007406
1105	58-59	-0.20325673433113423
1105	60-61	-0.1537654346932058
1105	62-63	-0.06677921366995321
1105	64-65	-0.055487499899832926
1105	66-67	-0.09287168937632373
1105	68-69	-0.13738027257458185
1105	70-71	-0.16508422563979508
1105	72-73	-0.16383181567278626
1105	74-75	-0.1592611591405202
1105	76-77	-0.1295130016888386
1105	78-79	-0.06210661621769731
1105	80-81	-0.015212297054098656
1105	82-83	0.07992365730934381
1105	84-85	0.05321707359370009
1105	86-87	-0.0397383720034199
1105	88-89	-0.07594264646888149
1105	90-91	-0.015787369495100734
1105	92-93	-0.08662562294189868
1105	94-95	-0.13111132642518442
1105	96-97	-0.0348236812863405
1105	98-99	-0.10141906615185903
1105	100	-0.17718262224991577
1106	1	-0.09437301721576574
1106	2	-0.058789612653612267
1106	3	-0.03180123207958019
1106	4	-0.06674774548308449
1106	5	-0.07360556703315524
1106	6	-0.046808236274202386
1106	7	-0.08993850615416221
1106	8	-0.045494715664233354
1106	9	-0.09777384537513001
1106	10-11	-0.07636981724344594
1106	12-13	-0.01484027029714241
1106	14-15	-0.0864464962613738
1106	16-17	-0.024116754544010632
1106	18-19	-0.06040504948081349
1106	20-21	-0.08779069722882582
1106	22-23	-0.011860361634617789
1106	24-25	-0.015391953587553076
1106	26-27	0.017288637221007264
1106	28-29	-0.10245472440885095
1106	30-31	-0.060226478398426764
1106	32-33	0.10097830039470068
1106	34-35	0.12969762558109466
1106	36-37	0.04795343541431407
1106	38-39	-0.06385597082282857
1106	40-41	-0.0534039867382603
1106	42-43	0.012088079790842698
1106	44-45	0.010005930432527066
1106	46-47	-0.014088024005538102
1106	48-49	-0.07178158226250986
1106	50-51	0.10426117747986297
1106	52-53	-0.0365654494148373
1106	54-55	0.08125649822022751
1106	56-57	0.0891483178949386
1106	58-59	-0.09909230990546547
1106	60-61	0.029473299090923888
1106	62-63	0.043571328066519754
1106	64-65	0.004202097332168364
1106	66-67	0.049684007890093085
1106	68-69	0.09545546926629811
1106	70-71	0.11403437235237845
1106	72-73	0.08972418517556946
1106	74-75	0.08717657831142134
1106	76-77	0.06262633306376131
1106	78-79	0.051835691722523336
1106	80-81	0.1361351949399605
1106	82-83	0.0657570589688703
1106	84-85	0.21396715228853225
1106	86-87	0.15786028089133453
1106	88-89	0.15357914605095146
1106	90-91	0.11159245393822914
1106	92-93	0.07181812441575275
1106	94-95	-0.03392659766470629
1106	96-97	-0.002677846152771224
1106	98-99	0.040694886616808645
1106	100	-0.0411277479550094
1107	1	-0.026218532902952063
1107	2	-0.055767605103305584
1107	3	0.006955855566026514
1107	4	-0.03996498298067763
1107	5	-0.06752785884974344
1107	6	-0.03830750059338328
1107	7	-0.0904057702678358
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2316	66-67	-0.1371807207902478
2316	68-69	-0.020565261061598505
2316	70-71	0.06924349209952396
2316	72-73	0.04082579496301264
2316	74-75	-0.01921785586203839
2316	76-77	-0.10697567653055273
2316	78-79	-0.20160860377941248
2316	80-81	-0.2485421278145168
2316	82-83	-0.24906005434144163
2316	84-85	-0.0981213841818942
2316	86-87	-0.2407061606015759
2316	88-89	-0.11754406040017784
2316	90-91	-0.11411057702868277
2316	92-93	-0.2157190324057332
2316	94-95	-0.16187005099789076
2316	96-97	-0.1086413452562276
2316	98-99	-0.036978007527888934
2316	100	-0.14544673920181594
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	17.0
13	111.0
14	434.0
15	1398.0
16	3159.0
17	6090.0
18	10515.0
19	15468.0
20	20946.0
21	28527.0
22	36739.0
23	41685.0
24	43893.0
25	45958.0
26	50740.0
27	58465.0
28	69878.0
29	85838.0
30	105305.0
31	132792.0
32	169000.0
33	227489.0
34	343355.0
35	651038.0
36	1125757.0
37	850244.0
38	949105.0
39	212627.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89372058371372	18.904434616021383	13.589952933941959	23.611891866322935
2	16.18957424560325	20.794512115248757	39.69264952967803	23.323264109469967
3	18.653353215110783	26.214153830555574	30.4630420065286	24.66945094780504
4	13.064708607266	17.394681136604078	37.701766933101005	31.83884332302892
5	13.408339662149956	37.12924877971273	34.50714202621123	14.955269531926083
6	30.17617862250032	36.70638283383901	18.656812971467915	14.460625572192756
7	26.623314104689648	32.60154526492837	22.697863764961753	18.077276865420238
8	25.52291924191109	34.765369421275786	20.96655484549479	18.745156491318333
9	26.50358037887685	16.015265388006313	20.76536521339936	36.71578901971748
10-11	24.68258974048327	25.924529347580705	28.778344195907092	20.614536716028933
12-13	25.276813439540163	24.007455650502955	28.549546283679973	22.166184626276912
14-15	22.892780864495307	25.38872777421497	26.586938550043605	25.13155281124612
16-17	22.26801869454455	27.910855516774625	27.216714245397295	22.604411543283533
18-19	22.034758632273668	26.881879685033173	29.229670104245255	21.853691578447904
20-21	22.929525349051723	26.236455085117576	29.11156669740093	21.72245286842977
22-23	22.677157028470212	26.145490970099004	28.9085174384705	22.268834562960283
24-25	22.344620938191124	26.22428350171866	29.06367204849013	22.367423511600087
26-27	22.246847556308147	26.293440891142833	29.0820077537644	22.37770379878462
28-29	22.223975181128576	26.44952991695515	29.14102491345554	22.18546998846074
30-31	22.752565083835556	26.20882141401866	29.10120196391271	21.937411538233075
32-33	22.497211864880505	26.387756362533327	28.908886931816085	22.20614484077008
34-35	22.704314466115484	26.54127867153721	28.784788566890846	21.96961829545646
36-37	22.6703720619526	26.4472289029912	28.682695278779985	22.199703756276215
38-39	22.526546583446766	26.624554814136086	28.619710072115	22.229188530302146
40-41	22.704015571606785	26.352693488847635	28.742874599573064	22.200416339972513
42-43	22.471229786985322	26.56123531224328	28.502263539861204	22.46527136091019
44-45	22.385742310252922	26.70446274935384	28.47658210721642	22.433212833176817
46-47	22.440104931302006	26.505975317760377	28.180848334690445	22.873071416247175
48-49	22.43119099429822	26.654751539904325	28.123946453443033	22.790111012354426
50-51	22.49372113120282	26.899958456173913	27.966495925829516	22.639824486793756
52-53	22.505488227621697	26.987580195277804	27.722335880476283	22.78459569662421
54-55	22.35857043050836	26.81144089136665	28.008830135651593	22.821158542473395
56-57	22.452738429259394	26.956928181857904	27.679615343655428	22.91071804522727
58-59	22.431332655137336	26.866681521908138	27.884732153564862	22.817253669389665
60-61	22.56126759597601	26.916401409806124	27.88657236747837	22.6357586267395
62-63	22.4281388754218	26.927074003447615	27.879244898989906	22.765542222140674
64-65	22.35698308435125	26.686850057457402	27.856503090404377	23.099663767786968
66-67	22.242838390500008	26.795049761438104	27.712874889566937	23.249236958494947
68-69	22.161383710521594	26.870558809664512	27.754398722132724	23.213658757681163
70-71	22.251516870520724	26.62398351185341	27.701393324805025	23.42310629282084
72-73	22.17903307833169	26.901240561576785	27.43110600117868	23.48862035891285
74-75	22.219376303718104	26.982148082892397	27.289668073630764	23.508807539758735
76-77	22.132096238899866	26.892200321752252	27.41233083486187	23.563372604486013
78-79	22.069743037778935	26.8473288687978	27.41929763249725	23.663630460926015
80-81	22.117902817096823	27.004163181900854	27.085518257461672	23.79241574354066
82-83	22.24511527553458	26.984147551019692	27.15011246705032	23.62062470639541
84-85	22.224217803192587	27.025689652922125	27.00792803651289	23.742164507372397
86-87	22.028727789843792	26.996686753710947	27.196113310147922	23.77847214629734
88-89	22.001373453198227	26.8432925083623	27.18611671666186	23.969217321777613
90-91	22.265388408666773	26.84304914583347	27.024108163279976	23.867454282219786
92-93	22.158034068228954	26.850891985939473	27.031117341403625	23.959956604427948
94-95	22.01460596274427	26.73891621702217	27.020331204301982	24.22614661593158
96-97	21.964912267427486	26.713930393901386	27.123117661957824	24.198039676713307
98-99	21.985369065221033	26.62237303294017	26.96286048044481	24.42939742139398
100	21.993903240664988	26.668829443015728	26.996310353992257	24.34095696232702
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1608.0
1	1184.5
2	1738.5
3	2440.0
4	1659.0
5	1252.5
6	1690.0
7	3046.0
8	5003.0
9	6028.5
10	5395.0
11	4606.0
12	4642.5
13	5059.5
14	5647.5
15	6189.0
16	6612.0
17	7049.0
18	7630.0
19	8363.0
20	9278.5
21	10510.5
22	12256.5
23	14896.5
24	18364.0
25	22585.5
26	28160.5
27	34693.0
28	41720.5
29	49711.5
30	58599.5
31	67193.0
32	76037.5
33	85564.0
34	95573.0
35	105677.5
36	118292.0
37	129767.5
38	137621.5
39	145789.0
40	152311.5
41	157536.0
42	163826.5
43	172113.5
44	179215.5
45	185073.5
46	191451.0
47	196897.5
48	202753.0
49	207671.5
50	208611.0
51	204992.0
52	198803.0
53	192650.0
54	184275.5
55	172262.0
56	157803.5
57	142103.0
58	126912.0
59	109607.5
60	90703.5
61	74484.0
62	60893.5
63	47814.5
64	36084.0
65	27750.0
66	21380.0
67	16518.5
68	12934.0
69	9923.5
70	7767.5
71	6122.0
72	4832.5
73	3977.0
74	3236.5
75	2383.0
76	1645.0
77	1076.0
78	671.0
79	448.0
80	291.0
81	199.0
82	131.0
83	81.0
84	56.5
85	37.5
86	26.0
87	20.0
88	14.0
89	12.5
90	8.5
91	5.5
92	3.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030377940545868513
2	0.07503691789879227
3	0.0027994615197936114
4	0.0
5	0.0012484085155836377
6	9.646793074964472E-4
7	0.0
8	3.026444886263364E-4
9	0.0
10-11	9.457640269573013E-6
12-13	4.728820134786506E-5
14-15	0.012124694825592603
16-17	0.0010403404296530313
18-19	2.9318684835676333E-4
20-21	2.7427156781761734E-4
22-23	0.0019009856941841754
24-25	8.890181853398631E-4
26-27	0.007480993453232252
28-29	0.008152485912371936
30-31	0.010384489015991167
32-33	0.009296860384990271
34-35	0.004917972940177966
36-37	0.003887090150794508
38-39	0.0033101740943505538
40-41	0.0035182421802811606
42-43	0.0018915280539146026
44-45	0.005532719557700213
46-47	0.018669381892137127
48-49	0.019161179186154925
50-51	0.01412971456274208
52-53	0.007868756704284746
54-55	0.0011822050336966266
56-57	0.0026859698365587358
58-59	0.002969699044645926
60-61	0.0035655303816290256
62-63	0.0034047504970462846
64-65	0.0038965477910640808
66-67	0.0029791566849154986
68-69	0.008001163668058768
70-71	4.5396673293950454E-4
72-73	0.005570550118778504
74-75	0.007717434459971578
76-77	0.0069135350370578726
78-79	0.004795023616673517
80-81	5.580007759048078E-4
82-83	0.0013524425585489407
84-85	9.457640269573013E-4
86-87	0.0013808154793576598
88-89	0.0010687133504617503
90-91	0.0010781709907313234
92-93	3.1210212889590944E-4
94-95	0.0010308827893834582
96-97	0.002912953203028488
98-99	0.0036411915037856094
100	0.005977228650370143
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5286731.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.35990804494617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52575127802407	48.48664564046026
2	14.8077290292545	18.764326992960896
3	4.303498824876677	8.180078694471604
4	1.6530923146187082	4.189591081761942
5	0.8259301612191824	2.616542953319748
6	0.47101063679691424	1.7905914381386414
7	0.29782213144452363	1.3208988003452422
8	0.21157344549600715	1.0724219241103556
9	0.1442923134246281	0.822811293915929
>10	0.7081517586853996	7.667684760848823
>50	0.034468521043393416	1.5050462093065102
>100	0.015407898848458548	1.7312193379029766
>500	7.266780317966139E-4	0.31437646819280585
>1k	4.3600658862350076E-4	0.5293475060880002
>5k	3.633388238525792E-5	0.2090547404880699
>10k+	7.266776477051584E-5	0.7993621576882779
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18555	0.35097303040385447	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16168	0.3058222557569129	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9081	0.17176966257598505	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.6745841617438074E-5
2	0.0	0.0	0.0	0.0	5.6745841617438074E-5
3	0.0	0.0	0.0	0.0	5.6745841617438074E-5
4	0.0	0.0	0.0	0.0	5.6745841617438074E-5
5	0.0	0.0	0.0	0.0	5.6745841617438074E-5
6	0.0	0.0	0.0	0.0	7.56611221565841E-5
7	0.0	0.0	0.0	0.0	7.56611221565841E-5
8	0.0	0.0	0.0	0.0	9.457640269573013E-5
9	0.0	0.0	0.0	3.783056107829205E-5	9.457640269573013E-5
10-11	0.0	0.0	0.0	3.783056107829205E-5	9.457640269573013E-5
12-13	0.0	0.0	0.0	3.783056107829205E-5	1.1349168323487615E-4
14-15	0.0	1.8915280539146026E-5	0.0	4.728820134786506E-5	1.4186460404359518E-4
16-17	0.0	3.783056107829205E-5	0.0	1.1349168323487615E-4	1.607798845827412E-4
18-19	0.0	3.783056107829205E-5	0.0	1.4186460404359518E-4	1.7969516512188725E-4
20-21	0.0	3.783056107829205E-5	0.0	2.0806808593060627E-4	1.8915280539146025E-4
22-23	0.0	3.783056107829205E-5	0.0	3.972208913220665E-4	1.9861044566103325E-4
24-25	0.0	3.783056107829205E-5	0.0	0.0010687133504617503	2.0806808593060627E-4
26-27	0.0	3.783056107829205E-5	0.0	0.0025913934338630054	2.269833664697523E-4
28-29	0.0	3.783056107829205E-5	0.0	0.006913535037057872	2.458986470088983E-4
30-31	0.0	3.783056107829205E-5	0.0	0.019132806265346202	2.7427156781761734E-4
32-33	0.0	4.728820134786506E-5	0.0	0.047571930555952255	3.4047504970462846E-4
34-35	0.0	5.6745841617438074E-5	0.0	0.093848164394973	3.5939033024377445E-4
36-37	0.0	5.6745841617438074E-5	0.0	0.16547087415644943	3.7830561078292045E-4
38-39	0.0	5.6745841617438074E-5	0.0	0.26600559022201054	4.3505145240035854E-4
40-41	0.0	5.6745841617438074E-5	0.0	0.3757047597087879	4.3505145240035854E-4
42-43	0.0	5.6745841617438074E-5	0.0	0.4926390240017886	4.539667329395046E-4
44-45	0.0	5.6745841617438074E-5	0.0	0.6353548156696454	4.539667329395046E-4
46-47	0.0	5.6745841617438074E-5	0.0	0.7999082987199462	4.539667329395046E-4
48-49	0.0	5.6745841617438074E-5	0.0	0.9733614212639152	4.728820134786506E-4
50-51	0.0	5.6745841617438074E-5	0.0	1.154352283102734	4.728820134786506E-4
52-53	0.0	5.6745841617438074E-5	0.0	1.335825484595301	5.012549342873697E-4
54-55	0.0	7.56611221565841E-5	0.0	1.552935074623619	5.107125745569427E-4
56-57	0.0	9.457640269573013E-5	0.0	1.7989188403949434	5.107125745569427E-4
58-59	0.0	9.457640269573013E-5	0.0	2.069246572220149	5.107125745569427E-4
60-61	0.0	9.457640269573013E-5	0.0	2.344785463833889	5.107125745569427E-4
62-63	0.0	9.457640269573013E-5	0.0	2.6255449728764333	6.052889772526728E-4
64-65	0.0	9.457640269573013E-5	0.0	2.92176772375973	6.052889772526728E-4
66-67	0.0	1.1349168323487615E-4	0.0	3.25645469761938	6.052889772526728E-4
68-69	0.0	1.1349168323487615E-4	0.0	3.633833459655882	6.052889772526728E-4
70-71	0.0	1.1349168323487615E-4	0.0	4.034203745187717	6.431195383309648E-4
72-73	0.0	1.1349168323487615E-4	0.0	4.4189216360734065	6.620348188701109E-4
74-75	0.0	1.1349168323487615E-4	0.0	4.81379703260862	6.620348188701109E-4
76-77	0.0	1.1349168323487615E-4	0.0	5.242256509741086	6.620348188701109E-4
78-79	0.0	1.1349168323487615E-4	0.0	5.711904010247542	6.620348188701109E-4
80-81	0.0	1.1349168323487615E-4	0.0	6.195870378122132	6.904077396788299E-4
82-83	0.0	1.1349168323487615E-4	0.0	6.696973990165189	7.755265021049869E-4
84-85	0.0	1.1349168323487615E-4	0.0	7.206116596437383	7.849841423745599E-4
86-87	0.0	1.1349168323487615E-4	0.0	7.73975449100777	7.94441782644133E-4
88	0.0	1.1349168323487615E-4	0.0	8.15220218316385	7.94441782644133E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9160	0.0	54.593647	1
GTATCAA	16445	0.0	40.764793	1
TACCTGG	5280	0.0	35.628605	2
TCAACGC	20485	0.0	32.230564	4
ATCAACG	20665	0.0	31.904343	3
CAACGCA	20810	0.0	31.817528	5
TATCAAC	21170	0.0	31.279118	2
AACGCAG	21465	0.0	30.911427	6
GTACCTG	6485	0.0	29.452375	1
ACCTGGG	6620	0.0	27.96828	3
ACGCAGA	24615	0.0	26.898142	7
TATCACG	595	0.0	26.874146	2
GTACATG	17525	0.0	26.119156	1
CGCAGAG	25415	0.0	25.94813	8
GTGGTAT	4255	0.0	25.097506	1
TACATGG	17970	0.0	24.758015	2
ACATGGG	18555	0.0	23.477201	3
GCAGAGT	28135	0.0	23.188944	9
CATGGGG	12930	0.0	22.27868	4
TGGTATC	4465	0.0	22.119257	2
>>END_MODULE