FastQCFastQC Report
Mon 6 Jun 2016
ERR523012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7266280
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231580.31870503201087763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185440.25520624033205436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95610.1315803960210727No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118550.053.637591
GTATCAA214550.041.5276681
TCAACGC266100.032.6586844
CAACGCA271050.032.027145
ATCAACG271300.032.000283
TATCAAC274800.031.8543322
TACCTGG69250.030.8885252
AACGCAG282250.030.8798946
TATCACG7000.027.53542
ACGCAGA324250.026.8514217
TATAACG6700.026.6633282
GTACCTG83950.026.0704021
GTGGTAT58600.026.0233271
CGCAGAG336550.025.8126248
GTACATG264200.025.7781141
TACATGG271300.024.6754882
ACCTGGG87650.023.8104533
ACATGGG278400.023.282613
TGGTATC62800.023.206392
GCAGAGT373500.022.9176279
Produced by FastQC (version 0.11.5)