FastQCFastQC Report
Mon 6 Jun 2016
ERR523012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7266280
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213490.2938092118663195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178840.2461231882063449No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90420.12443781412221934No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117050.053.179561
GTATCAA215600.041.0484121
TCAACGC260450.033.4316024
TACCTGG76400.032.9357222
ATCAACG267400.032.527983
CAACGCA268550.032.388245
TATCAAC273650.031.9170822
AACGCAG278700.031.4112326
GTACCTG94600.027.3544451
ACGCAGA324650.026.9219597
ACCTGGG93000.026.5775453
CGCAGAG340200.025.6975868
TATAACG6150.025.2374572
GTACATG260600.024.68031
GTGGTAT61250.024.0433251
TACATGG268400.023.6569082
TATCACG7400.023.5167182
GCAGAGT374700.022.9302719
ACATGGG277300.022.4870833
TGGTATC63450.021.9415252
Produced by FastQC (version 0.11.5)