FastQCFastQC Report
Mon 6 Jun 2016
ERR523011_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523011_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8654702
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT660710.763411611399214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346460.40031418759421183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265970.3073127185661621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164270.18980433988368403No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC142770.1649623522566115No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC125460.14496166361360566No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA105370.12174884819835506No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG102170.1180514360864187No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG72800.034.094982
TATCACG7350.030.0606522
GTACATG313250.028.590851
GTACCTG90400.028.3433551
TACATGG325450.026.9246182
ACCTGGG91700.026.6587353
TATAACG7700.026.2521932
ACATGGG324250.026.0249563
CATGGGG217850.024.2536164
ATGGGGG132250.023.7798025
TAACGCA9500.022.266744
GAGTACT278100.020.47600612-13
AGAGTAC423300.019.51103210-11
GGGTTAG29050.018.4493831
AGTACTT294700.018.42982912-13
ATAACGC12050.017.9463583
GTACTTT315450.017.85823414-15
TGGGGGG141000.017.6936136
CATGGGA116050.017.4582164
CCTGGGG90300.016.554164
Produced by FastQC (version 0.11.5)