FastQCFastQC Report
Mon 6 Jun 2016
ERR523011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8654702
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT668600.7725280431377072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316710.3659398093660533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256570.29645157048734894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156610.18095365964073631No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC148000.1710053101770575No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC128210.148139127147301No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA108120.1249263117320504No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG107170.12382864251131928No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA99240.11466599312142695No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC87510.10111266684861016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG75300.036.6395342
TATAACG6600.030.6218452
GTACCTG95000.029.9051861
GTACATG309850.027.9318371
ACCTGGG98350.027.4278433
TACATGG317950.026.8450282
ACATGGG323550.025.4621243
CATGGGG216600.023.9966624
ATGGGGG134000.022.4101725
TAACGCA9350.021.1101744
GAGTACT293350.020.4172912-13
GGGTTAG29350.020.3530161
AGAGTAC434750.019.55103910-11
GTACTTT327450.018.18773514-15
AGTACTT309700.018.12499412-13
ATAACGC11400.017.7262843
CCTGGGG91050.017.7038754
GTATTAC19650.016.9953061
TGGGGGG134700.016.9207956
GTTAGAG38350.016.78843
Produced by FastQC (version 0.11.5)