FastQCFastQC Report
Mon 6 Jun 2016
ERR523010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6595420
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165400.25078008678749797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134480.2038990693541882No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT132770.2013063610808713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77650.11773321486728669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG76050.038.4675452
ACCTGGG90400.031.6841663
TATAACG4800.028.4158122
GTACATG250500.027.4122771
TACATGG251850.026.8733712
CATGGGG171600.026.4208354
ATGGGGG110500.026.1322275
ACATGGG253350.025.8411453
GTACCTG125150.023.8263991
TGGGGGG114050.023.535496
ATAACGC6850.021.9711513
CGTATCA29850.021.56573394
CCTGGGG93800.021.3094834
CCGTATC40200.020.80568394
TAACGCA6650.019.8026474
GTATCAA341400.019.4385341
GAGTACT150000.019.1908912-13
GGTATCA270400.018.6461051
GTACTTT160000.017.75714114-15
TCGCCGT78700.017.7324994
Produced by FastQC (version 0.11.5)