FastQCFastQC Report
Mon 6 Jun 2016
ERR523010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6595420
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT278940.42292985132106825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152350.23099362891218453No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT147810.22411006425671148TruSeq Adapter, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133060.20174606014476712No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT129470.19630288897447015No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT77540.11756643246373999RNA PCR Primer, Index 11 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76080.11535277510757463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG73500.040.225942
ACCTGGG89050.032.1452183
GTACATG249750.027.6887861
TACATGG251650.027.1774672
TGGGGGG100250.026.5824816
CATGGGG170500.026.4103494
ACATGGG253600.026.1894253
ATGGGGG108050.025.9710315
GTACCTG119000.025.1421411
TATAACG5550.023.7141722
CCTGGGG86500.023.5834124
GTATACG4950.021.8582151
TAACGCA5900.020.7135414
GTATCAA319400.020.2221661
ATAACGC6150.019.8715293
GGTATCA251600.019.781861
TATCACG4850.019.3834532
GAGTACT158450.019.04231812-13
GTACTTT163300.018.37589514-15
CTGGGGG58650.018.2728565
Produced by FastQC (version 0.11.5)