FastQCFastQC Report
Mon 6 Jun 2016
ERR523009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6553813
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223860.3415721504412775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177400.2706821204694122No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT115910.17685887589407875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99500.15182001683600066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG68800.029.8050612
GTACCTG80750.025.5692061
GTACATG249550.025.0097081
ACCTGGG81250.024.8333
TACATGG252850.024.38552
GTATCAA349800.023.3681961
ACATGGG260350.022.7980143
GGTATCA263600.022.3385091
CATGGGG149300.021.2633934
TCAACGC388300.020.857124
GAGTACT192850.020.73981912-13
CAACGCA391200.020.7256015
ATCAACG394550.020.4435983
AACGCAG401000.020.2914186
TATCAAC402800.020.0947862
GTACTTT205700.019.34220314-15
ACGCAGA436400.018.5361427
AGTACTT202900.018.29953612-13
GTATAGG19550.018.2836171
AGAGTAC317100.018.24331710-11
Produced by FastQC (version 0.11.5)