FastQCFastQC Report
Mon 6 Jun 2016
ERR523009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6553813
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207740.3169757818845304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179160.27336758006369727No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT116880.1783389303295654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98460.1502331543484686No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG65100.030.1831282
GTACCTG80250.025.3234351
ACCTGGG80300.023.707293
GTACATG247250.023.4400371
TAACGCA5850.023.301364
TACATGG250800.022.6978932
GTATCAA348400.022.2786921
GGTATCA265850.021.6231231
ACATGGG258100.021.1803993
GAGTACT194950.020.29870412-13
TCAACGC388250.019.758224
CATGGGG147750.019.5971224
TATAACG6250.019.5551992
CAACGCA392300.019.5182955
ATCAACG396250.019.2882863
ATAACGC7350.019.1856423
GTACTTT204300.019.09322414-15
TATCAAC404450.019.0494772
AACGCAG404300.019.0062646
AGTACTT204000.018.29236212-13
Produced by FastQC (version 0.11.5)