FastQCFastQC Report
Mon 6 Jun 2016
ERR523008_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523008_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868292
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86400.3012245615160521No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69200.24125856084387504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68870.24010805036586233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34720.12104764786848758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2250.031.3511072
TACCTGG28350.026.2088622
GTACATG114100.024.2749651
TACATGG116100.023.4930782
ACATGGG118350.022.2123
ACCTGGG32950.021.8363573
CATGGGG75400.020.9561774
GTATCAA172800.020.8455831
GAGTACT76450.020.5949812-13
GCCGTAT17850.019.21676394
GTACTTT82150.018.79460714-15
TCAACGC190150.018.6721674
GTACCTG40750.018.579211
CAACGCA190400.018.547245
CGTATCA10400.018.52448394
ATCAACG194000.018.3016133
CGCCGTA19900.018.18164894
TATCAAC195800.018.18142
AGAGTAC135300.018.09672210-11
AACGCAG195450.018.062666
Produced by FastQC (version 0.11.5)