FastQCFastQC Report
Mon 6 Jun 2016
ERR523008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868292
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT141240.4924184845894351No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82060.2860936055324911No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72110.25140397142271426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70360.24530277949385906No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT70260.24495413995506732RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT40350.1406760539024618RNA PCR Primer, Index 17 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35340.12320921300899629No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG30250.030.4540122
ACCTGGG34050.025.3984343
GTACATG112600.022.848921
TACATGG114600.022.0653822
ACATGGG115850.021.5429273
GTACCTG44650.021.2787761
CATGGGG73500.020.3990334
GTATCAA176300.020.355841
GAGTACT78200.020.25345412-13
GTACTTT84450.018.5597514-15
ATAACGC3801.2732926E-1118.552993
GGTATCA137450.018.444241
TCAACGC192750.018.3614444
AGTACTT84100.018.30169312-13
CAACGCA193850.018.2578895
ATACCGT3358.440111E-1018.2385236
TACCGTA2603.6708116E-718.0766417
ATCAACG196550.018.0064533
AACGCAG199350.017.9179556
TATCAAC200150.017.8237882
Produced by FastQC (version 0.11.5)