FastQCFastQC Report
Mon 6 Jun 2016
ERR523007_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523007_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7046563
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224710.31889305467076645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187000.26537760323720944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108350.153762905405089No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA99890.14175705233884947No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG144950.058.486642
GTACCTG164000.052.484591
ACCTGGG165650.050.4698333
CCTGGGG136600.040.0977864
ATAACGC9450.030.8497333
GTACCCG13750.029.076411
TATAACG9450.028.8586062
CTGGGGG83750.027.5629945
TAACGCA10400.026.220434
TATCACG7800.025.9210762
CATGGGG174250.024.5805034
TGGGGGG116650.022.9226886
ATGGGGG108650.022.198185
GTACATG355500.021.7910691
TACCCGG16850.021.4867132
TACATGG365650.020.9733372
ACATGGG362400.020.6689833
GTATCAA388250.020.4980931
GAGTACT200150.019.6628812-13
GGTATCA302550.019.3551581
Produced by FastQC (version 0.11.5)