FastQCFastQC Report
Mon 6 Jun 2016
ERR523007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7046563
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199020.2824355646859327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183470.26036806880176905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102920.14605702099023313No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA95400.1353851515980202No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG149100.058.312792
GTACCTG169900.051.9155161
ACCTGGG164500.051.8475273
CCTGGGG135850.040.298014
GTACCCG14100.032.345241
CTGGGGG81800.029.872215
TATAACG8600.029.5097142
ATAACGC9250.026.9247363
TATCACG7450.025.2332632
TAACGCA9650.024.3478164
TACCCGG17400.024.0386712
TGGGGGG106950.022.5399996
CATGGGG182800.022.3645614
ATGGGGG104450.020.9649965
GTATCAA419950.020.6117081
GAGTACT214950.020.19489512-13
GTACATG381500.019.7558981
GTACACG16000.019.6884981
TACCGTA4300.019.670777
GGTATCA329950.019.4367681
Produced by FastQC (version 0.11.5)