FastQCFastQC Report
Mon 6 Jun 2016
ERR523006_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523006_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11007508
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359810.32687689166339917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282810.2569246372566797No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT232290.21102869059918014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185900.16888472849622277No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA128360.11661131656683785No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114400.10392906368998324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG221300.056.814882
ACCTGGG245200.050.5862463
GTACCTG255350.049.9853971
CCTGGGG217650.038.6659744
CTGGGGG130300.030.1269265
TATAACG11100.027.9578022
GTACCCG21100.026.9694521
TGGGGGG193200.026.268876
ATAACGC12450.025.681343
CATGGGG284900.025.1493664
ATGGGGG178650.023.9470025
GTACATG525950.023.8388291
TACATGG538500.022.9642432
GTACACG24800.022.5665151
TAACGCA14150.022.2614234
ACATGGG536500.022.1819923
TACCGGG15400.020.7620532
GTACCGG19050.020.4904881
TACCCGG27000.020.201162
TACACGG24700.020.1785982
Produced by FastQC (version 0.11.5)