FastQCFastQC Report
Mon 6 Jun 2016
ERR523006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523006_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11007508
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312680.28406066114146816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268280.2437245560030481No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT232960.21163736605960223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174620.15863717746105657No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA123080.11181459055037708No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG233550.059.5442162
ACCTGGG260000.052.397663
GTACCTG270900.051.6157261
CCTGGGG226300.040.8882144
CTGGGGG132750.033.4529385
TATAACG12250.029.1576142
TGGGGGG171900.028.9233596
GTACCCG22900.026.2799531
TAACGCA13950.025.9390164
ATAACGC14150.024.908173
CATGGGG281250.024.695564
ATGGGGG169950.024.2225535
GTACATG534350.022.4985751
TATCACG10100.021.870112
TACATGG545300.021.7965532
ACATGGG538850.021.1747253
GTACACG23550.021.1624071
TACCCGG28100.020.0700932
GTATAAC28400.018.8728121
GAGTACT320050.018.4463912-13
Produced by FastQC (version 0.11.5)