FastQCFastQC Report
Mon 6 Jun 2016
ERR523005_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523005_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12487475
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438220.3509276294847437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351420.2814179808167784No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT286550.22946992886872644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216690.17352587292467053No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124960.10006826840494175No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG168850.045.2268562
GTACCTG191600.040.2363781
ACCTGGG204000.036.8350753
CCTGGGG191650.026.0299114
CATGGGG309150.022.569964
GTACATG559350.022.3935491
TACATGG570450.021.4899272
ACATGGG574800.020.7384433
TATCACG12100.020.5969052
GAGTACT368600.020.11650812-13
GTACACG30150.019.1890371
ATGGGGG170800.018.9940175
GTATCAA819700.018.1788181
TACACGG26350.018.0240442
AGTACTT391350.017.81843212-13
CTGGGGG121800.017.6410565
TATAACG10450.017.5493012
GTACTTT428650.017.18915714-15
TCAACGC893200.016.3921534
CAACGCA897800.016.3077745
Produced by FastQC (version 0.11.5)