FastQCFastQC Report
Mon 6 Jun 2016
ERR523005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12487475
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378910.3034320389029808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343490.2750676177529885No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT296100.23711759182701067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215240.17236470943885773No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG170850.046.131352
GTACCTG198450.040.3251651
ACCTGGG206350.037.6898733
CCTGGGG192750.026.9888484
ATAACGC13700.020.9237923
GTACATG558650.020.6034181
CATGGGG304850.020.4711884
TATAACG12500.020.3030782
TACATGG572600.019.5838092
GAGTACT395750.019.34747712-13
ACATGGG569050.019.0514623
ATGGGGG168450.018.4957495
TAACGCA13850.018.3220944
GTATAGG34600.018.073541
AGTACTT414200.017.49840512-13
CTGGGGG121800.017.3232145
GTATCAA836150.017.1620061
GTACTTT446000.017.0187614-15
GTACACG28300.016.2819841
GGTATCA683600.016.2528441
Produced by FastQC (version 0.11.5)