##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523005_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12487475
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04665658990308	33.0	31.0	34.0	30.0	34.0
2	32.18249077575731	34.0	31.0	34.0	30.0	34.0
3	32.35926358210927	34.0	31.0	34.0	30.0	35.0
4	35.47276531084146	37.0	35.0	37.0	33.0	37.0
5	35.413463170096435	36.0	35.0	37.0	32.0	37.0
6	35.55016870904647	37.0	35.0	37.0	33.0	37.0
7	35.39132170434775	36.0	35.0	37.0	33.0	37.0
8	35.38102410615436	36.0	35.0	37.0	33.0	37.0
9	36.639153311618244	37.0	35.0	39.0	33.0	39.0
10-11	36.44314022650696	37.5	35.0	39.0	32.0	39.0
12-13	36.45346945639531	37.0	35.0	39.0	33.0	39.0
14-15	37.577648884181954	38.0	37.0	41.0	33.0	41.0
16-17	37.39437276150703	38.0	36.5	41.0	32.0	41.0
18-19	37.2805308118735	38.0	36.0	40.0	32.0	41.0
20-21	37.30312340965648	38.0	36.0	40.0	32.5	41.0
22-23	37.14317137772048	38.0	35.5	40.0	32.0	41.0
24-25	37.05755426937792	38.0	36.5	40.0	31.5	41.0
26-27	36.73618089325504	38.0	36.0	40.0	31.5	41.0
28-29	36.67016490523504	38.0	35.5	40.0	31.0	41.0
30-31	36.43478028985043	37.5	35.5	40.0	31.0	41.0
32-33	36.37909985805777	37.5	36.0	40.0	30.0	41.0
34-35	36.142904870680425	37.5	35.0	40.0	30.0	41.0
36-37	35.91482069033171	37.0	35.0	40.0	30.0	41.0
38-39	35.8671154897207	37.0	34.5	40.0	30.0	41.0
40-41	36.057031305367985	37.5	35.0	40.0	30.0	41.0
42-43	36.08517130164425	37.5	35.0	40.0	30.0	41.0
44-45	35.92687953329236	37.0	35.0	40.0	30.0	41.0
46-47	35.81261524047095	37.0	35.0	40.0	28.5	41.0
48-49	35.59182004368377	37.5	34.5	40.0	28.5	41.0
50-51	35.423058384501275	37.5	34.0	40.0	27.5	41.0
52-53	35.41194088476654	37.5	34.0	40.0	27.5	41.0
54-55	35.21526385438209	37.5	34.0	40.0	26.5	41.0
56-57	34.968190807188805	37.0	34.0	40.0	26.5	41.0
58-59	34.77025107157372	37.0	34.0	39.0	26.5	41.0
60-61	34.56754848358055	36.5	33.0	39.0	26.0	41.0
62-63	34.33257952468374	36.0	33.0	39.0	26.0	40.5
64-65	34.131746169662	36.5	33.0	38.0	25.0	40.0
66-67	33.64350014714744	35.5	33.0	38.0	25.0	40.0
68-69	33.50638800077678	35.5	32.5	37.0	23.0	40.0
70-71	33.07726994448437	35.0	32.0	37.0	24.0	39.5
72-73	32.847621516759794	35.0	32.0	37.0	23.5	39.0
74-75	32.631037980056014	35.0	31.5	37.0	22.5	39.0
76-77	31.111115778009562	33.5	29.5	36.0	21.5	37.0
78-79	32.243348955653566	35.0	31.5	36.5	23.0	37.0
80-81	32.34346687380756	35.0	32.0	36.0	23.0	37.5
82-83	32.247050264364894	35.0	31.5	37.0	23.5	37.0
84-85	31.914460249169668	35.0	31.0	35.5	22.0	36.5
86-87	31.78829351009712	35.0	31.0	36.0	22.5	36.0
88-89	31.713282749314814	35.0	31.0	35.5	22.0	37.0
90-91	31.465504315323955	34.0	31.0	35.0	21.5	36.5
92-93	31.23850570271412	34.0	30.5	35.0	21.0	36.5
94-95	31.338289365944675	34.0	30.0	35.0	20.5	36.5
96-97	31.063390797579174	34.0	30.0	35.0	20.0	37.0
98-99	30.90931709572992	34.0	30.0	35.0	20.0	36.0
100	29.646495628619878	33.0	29.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.09043893110565193
1101	2	-0.16184050209412248
1101	3	-0.14478481829623746
1101	4	-0.10308220177676475
1101	5	-0.22808119799265114
1101	6	-0.12402870564902457
1101	7	-0.13042489880412944
1101	8	-0.22105971888630904
1101	9	-0.15768414732297487
1101	10-11	-0.18286671470298188
1101	12-13	-0.16957941794694165
1101	14-15	-0.3365100121564808
1101	16-17	-0.2826981773314827
1101	18-19	-0.44270871893968433
1101	20-21	-0.36575715746648285
1101	22-23	-0.8324642095686485
1101	24-25	-0.3391933311847737
1101	26-27	-0.35158496545555096
1101	28-29	-0.42665221936086084
1101	30-31	-0.4199404521376451
1101	32-33	-0.36152409164980526
1101	34-35	-0.4253648597795632
1101	36-37	-0.4858534549300586
1101	38-39	-0.3356682101010833
1101	40-41	-0.39458386810176194
1101	42-43	-0.389654561668344
1101	44-45	-0.5382005593119956
1101	46-47	-0.3719447718234221
1101	48-49	-0.4972529722334258
1101	50-51	-0.5368121331830906
1101	52-53	-0.47339724536779926
1101	54-55	-0.5360695807872844
1101	56-57	-0.6860154669637666
1101	58-59	-0.6088339014305859
1101	60-61	-0.8028945550554099
1101	62-63	-0.623283750547337
1101	64-65	-0.5966633013081051
1101	66-67	-0.5495249960160535
1101	68-69	-0.559188221797811
1101	70-71	-0.6533684579655556
1101	72-73	-0.6438944211244149
1101	74-75	-0.6199557041897492
1101	76-77	-0.6796860100625892
1101	78-79	-0.5234260668134496
1101	80-81	-0.47834669853758527
1101	82-83	-0.41035562704722395
1101	84-85	-0.4986248762539027
1101	86-87	-0.6165460000952443
1101	88-89	-0.6095453063688474
1101	90-91	-0.5975576557392941
1101	92-93	-0.5872909062397262
1101	94-95	-0.6742083842351114
1101	96-97	-0.7277849244255066
1101	98-99	-0.7441936586175331
1101	100	-0.6137641253364663
1102	1	-0.04630850507168205
1102	2	-0.13991613138175296
1102	3	-0.1664607189436822
1102	4	-0.1480173373832514
1102	5	-0.14473722573777792
1102	6	-0.2157891831199521
1102	7	-0.14003218061550626
1102	8	-0.19398689276692238
1102	9	-0.19058642034731577
1102	10-11	-0.3157755466305261
1102	12-13	-0.22498593047471616
1102	14-15	-0.3765100164644579
1102	16-17	-0.3814824539280792
1102	18-19	-0.34715448138742033
1102	20-21	-0.3955211442916635
1102	22-23	-0.3254750179015886
1102	24-25	-0.32300092491561117
1102	26-27	-0.2956590215398123
1102	28-29	-0.39041389143530125
1102	30-31	-0.47791424933729587
1102	32-33	-0.4397691179853993
1102	34-35	-0.3884307424365119
1102	36-37	-0.42700347872986555
1102	38-39	-0.39161521287079637
1102	40-41	-0.44114297665383617
1102	42-43	-0.4697586295991911
1102	44-45	-0.31796201699251725
1102	46-47	-0.3198646571363071
1102	48-49	-0.3776647844770551
1102	50-51	-0.2428690523287642
1102	52-53	-0.3827586203637807
1102	54-55	-0.3193126062445515
1102	56-57	-0.36820857967024523
1102	58-59	-0.34659781516366905
1102	60-61	-0.4547965167203216
1102	62-63	-0.5650871875777668
1102	64-65	-0.5480821801865545
1102	66-67	-0.6121390251689647
1102	68-69	-0.4546314964934055
1102	70-71	-0.49386629271975124
1102	72-73	-0.5371038705258542
1102	74-75	-0.5976245881390554
1102	76-77	-0.483131825175402
1102	78-79	-0.46177558254417406
1102	80-81	-0.5197083196629535
1102	82-83	-0.4877413365433867
1102	84-85	-0.42522328581141977
1102	86-87	-0.5328438226937493
1102	88-89	-0.5218878913456813
1102	90-91	-0.53268226251474
1102	92-93	-0.5343080969203946
1102	94-95	-0.7013472301847834
1102	96-97	-0.7681843146018785
1102	98-99	-0.8507897504805193
1102	100	-0.8820191307542906
1103	1	-0.051592211465671056
1103	2	-0.09659818516747976
1103	3	-0.20740933548484008
1103	4	-0.04879519715891689
1103	5	-0.12469451048630731
1103	6	-0.09372180362717586
1103	7	-0.10872083693397627
1103	8	-0.10760477087518439
1103	9	-0.20406236690682533
1103	10-11	-0.26332809837063564
1103	12-13	-0.22150656729859008
1103	14-15	-0.21929171091565536
1103	16-17	-0.2631353564028771
1103	18-19	-0.3228555478696862
1103	20-21	-0.31165354113463195
1103	22-23	-0.3794162792530784
1103	24-25	-0.3116757906392209
1103	26-27	-0.36698189475939813
1103	28-29	-0.310341640486385
1103	30-31	-0.4481984217270636
1103	32-33	-0.2953868326008049
1103	34-35	-0.3878107485908089
1103	36-37	-0.4024128777996694
1103	38-39	-0.36758034363044345
1103	40-41	-0.44068919482887736
1103	42-43	-0.4224777715744281
1103	44-45	-0.34973380154102784
1103	46-47	-0.4539534008384294
1103	48-49	-0.4585952666156956
1103	50-51	-0.45528666382845273
1103	52-53	-0.43981310730652723
1103	54-55	-1.158122000390371
1103	56-57	-0.5544619646546991
1103	58-59	-0.5064905147482222
1103	60-61	-0.457838539938912
1103	62-63	-0.4369861159383319
1103	64-65	-0.432233132096961
1103	66-67	-0.4412184753584043
1103	68-69	-0.42173281138330765
1103	70-71	-0.4313370754013306
1103	72-73	-0.5520603669562689
1103	74-75	-0.609322759069606
1103	76-77	-0.5358996961137024
1103	78-79	-0.47304374463553955
1103	80-81	-1.2264363592430385
1103	82-83	-0.27289270493537643
1103	84-85	-0.19791533625253166
1103	86-87	-0.30054375871619854
1103	88-89	-0.2878204517775167
1103	90-91	-0.32457699108402593
1103	92-93	-0.354490785783959
1103	94-95	-0.33128900231428915
1103	96-97	-0.4468341845722499
1103	98-99	-0.3798444435724022
1103	100	-0.43187469263513734
1104	1	-0.06679557590333118
1104	2	-0.23196664327617356
1104	3	-0.14591695865923526
1104	4	-0.03900867138542452
1104	5	-0.07654809504700211
1104	6	-0.2547318650407746
1104	7	-0.25894994705379304
1104	8	-0.16399455783412975
1104	9	-0.22681068005100968
1104	10-11	-0.1798325375342884
1104	12-13	-0.22310273650608536
1104	14-15	-0.2900748589462552
1104	16-17	-0.313823236728318
1104	18-19	-0.2797745231143196
1104	20-21	-0.37494005792783724
1104	22-23	-0.3225869987702552
1104	24-25	-0.1835901228520953
1104	26-27	-0.28572605850878574
1104	28-29	-0.4249208064450869
1104	30-31	-0.4243992316712948
1104	32-33	-0.270913628296519
1104	34-35	-0.32575479938846286
1104	36-37	-0.42554634388532264
1104	38-39	-0.2759829442394519
1104	40-41	-0.17854526942346638
1104	42-43	-0.17886642868977987
1104	44-45	-0.2815468770619063
1104	46-47	-0.3305013086610842
1104	48-49	-0.37887012030945044
1104	50-51	-0.2878581662388555
1104	52-53	-0.3161761918872372
1104	54-55	-0.34827522512919273
1104	56-57	-0.37216298500680267
1104	58-59	-0.41227893463449306
1104	60-61	-0.45013540633061666
1104	62-63	-0.4068422252575985
1104	64-65	-0.6022541108860437
1104	66-67	-0.4573937466224933
1104	68-69	-0.3257211361696477
1104	70-71	-0.2700349643735507
1104	72-73	-0.33109264653346315
1104	74-75	-0.42552448988394076
1104	76-77	-0.3094999330844601
1104	78-79	-0.33391686383854235
1104	80-81	-0.3376776463440052
1104	82-83	-0.363225512263746
1104	84-85	-0.9435038510483764
1104	86-87	-0.4130934346149182
1104	88-89	-0.4057471906792145
1104	90-91	-0.4303029453032252
1104	92-93	-0.34939172752831027
1104	94-95	-0.3448360072002039
1104	96-97	-0.2393240366800775
1104	98-99	-0.37026332567632636
1104	100	-1.48039726033646
1105	1	-0.025564085759747
1105	2	-0.14846421213336214
1105	3	-0.10739134474319201
1105	4	-0.05386201846864225
1105	5	-0.14163437164471304
1105	6	-0.1389778379093869
1105	7	-0.1488668397593571
1105	8	-0.1978917393880124
1105	9	-0.20546050646993308
1105	10-11	-0.19042405011661145
1105	12-13	-0.17295670334176805
1105	14-15	-0.28043255185470173
1105	16-17	-0.27880123299636494
1105	18-19	-0.32172672323558515
1105	20-21	-0.28617454438145984
1105	22-23	-0.2918077666922656
1105	24-25	-0.2444610447045008
1105	26-27	-0.2707217370369719
1105	28-29	-0.28715834463376666
1105	30-31	-0.36761159178610825
1105	32-33	-0.27310956749023063
1105	34-35	-0.36492634989493666
1105	36-37	-0.3490172197181636
1105	38-39	-0.32545789465084596
1105	40-41	-0.2777998331697944
1105	42-43	-0.33595625584769806
1105	44-45	-0.4475357752629421
1105	46-47	-0.4156666750792297
1105	48-49	-0.35638556793364984
1105	50-51	-0.2581392550549282
1105	52-53	-0.4849397269284452
1105	54-55	-1.383768193709919
1105	56-57	-0.37906052094488274
1105	58-59	-0.3731478443908003
1105	60-61	-0.3219346636353819
1105	62-63	-0.25720726450018816
1105	64-65	-0.25085025229709146
1105	66-67	-0.2994998065923653
1105	68-69	-0.12498659345123997
1105	70-71	-0.15752954531842533
1105	72-73	-0.32476795334453357
1105	74-75	-0.3200684847512463
1105	76-77	-0.2910958376795314
1105	78-79	-0.29568061554117975
1105	80-81	-0.18771123390461497
1105	82-83	-0.2136787142341916
1105	84-85	-0.22410858837578118
1105	86-87	-0.19529408997376052
1105	88-89	-0.3565199598642472
1105	90-91	-0.28594543378574144
1105	92-93	-0.15375872848061434
1105	94-95	-0.26070540234530526
1105	96-97	-0.2591722594684889
1105	98-99	-0.16057550288858025
1105	100	-0.06001450524281893
1106	1	0.019806051110634826
1106	2	-0.07637843592121385
1106	3	-0.07872973053837029
1106	4	-0.05918793143769108
1106	5	-0.08483769297642141
1106	6	-0.1043911935924342
1106	7	-0.04552736457775808
1106	8	-0.13388676582022185
1106	9	-0.08494131008695405
1106	10-11	-0.10853272613924503
1106	12-13	-0.08361424531637596
1106	14-15	-0.09733259902903058
1106	16-17	-0.07629900640075249
1106	18-19	-0.1577331784986029
1106	20-21	-0.15775521166307271
1106	22-23	-0.11501483603203866
1106	24-25	-0.1039526416769121
1106	26-27	-0.049746285517095146
1106	28-29	-0.008048541939153608
1106	30-31	-0.12292706155968602
1106	32-33	-0.006318604858456922
1106	34-35	0.003484488203113756
1106	36-37	-0.12930807292496382
1106	38-39	-0.15449749352320907
1106	40-41	-0.06537717717435498
1106	42-43	-0.10280954370347217
1106	44-45	-0.12577992426896145
1106	46-47	-0.2305060213825314
1106	48-49	-0.1406050598451145
1106	50-51	-0.07338335380651273
1106	52-53	-0.15757592860110492
1106	54-55	-0.24489534877402974
1106	56-57	-0.15405950126622514
1106	58-59	-0.23466246113590472
1106	60-61	-0.1502751909178599
1106	62-63	-0.18547381725680623
1106	64-65	-0.1709172268667274
1106	66-67	-0.14294941031184294
1106	68-69	-0.007677173662983705
1106	70-71	-0.05858788098885981
1106	72-73	-0.03182533580184099
1106	74-75	-0.15624162586408374
1106	76-77	-0.12310891680108327
1106	78-79	-0.04191102401246383
1106	80-81	0.03913382843520452
1106	82-83	0.009867034321807466
1106	84-85	0.05813882230506451
1106	86-87	0.09368046345075243
1106	88-89	-0.02887680524756675
1106	90-91	-0.028786206666502778
1106	92-93	0.10492533810813143
1106	94-95	0.03477049579057834
1106	96-97	0.14466307458569716
1106	98-99	0.013229649325612769
1106	100	0.04608102032939243
1107	1	-0.007713324926250209
1107	2	-0.09252859185400553
1107	3	-0.11128655204510807
1107	4	-0.030501170654858356
1107	5	-0.11245533018432496
1107	6	-0.17642024030866565
1107	7	-0.18276679617163438
1107	8	-0.17835353507217633
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2308	84-85	0.23486895849319112
2308	86-87	0.2674592423242679
2308	88-89	0.18378649008709047
2308	90-91	0.2078824322058317
2308	92-93	0.23769343541816923
2308	94-95	0.13233310608903182
2308	96-97	0.12893521665689534
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2308	100	0.06003362684057478
2309	1	0.15078816932366124
2309	2	0.025808497072048908
2309	3	0.06024170579900101
2309	4	0.11331659953972917
2309	5	0.0957679287763682
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2309	7	0.07776660826379356
2309	8	0.09003401862060656
2309	9	0.16425522011284954
2309	10-11	0.11690053656528931
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2309	84-85	0.27617915057859577
2309	86-87	0.2899486094188326
2309	88-89	0.33207445428606874
2309	90-91	0.3343577820836856
2309	92-93	0.4662573136026822
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2310	2	0.07991542144528552
2310	3	0.08376220195783901
2310	4	0.16503071995175844
2310	5	0.15341857100856515
2310	6	0.08574666852280188
2310	7	0.11637792813446168
2310	8	0.08907043578886231
2310	9	0.17396955314840312
2310	10-11	0.19279516447006984
2310	12-13	0.18655408149684405
2310	14-15	0.18828464066925932
2310	16-17	0.24032971257884128
2310	18-19	0.25262213627264885
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2311	98-99	0.29145804418256205
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2312	1	0.12114011881364917
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2312	7	0.09253013589837167
2312	8	0.1212292957730412
2312	9	0.15644144828787176
2312	10-11	0.14252373080298497
2312	12-13	0.12068504733440477
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2312	22-23	0.1267797671164388
2312	24-25	0.19774463515781093
2312	26-27	-2.4285895450035255
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2312	30-31	0.038725050702218766
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2312	36-37	-1.4542966332908236
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2312	44-45	-0.029386310055173226
2312	46-47	0.036694893644650506
2312	48-49	0.13197209742165228
2312	50-51	-1.4207880133534303
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2312	54-55	0.16920672637859724
2312	56-57	0.2639381267069538
2312	58-59	0.3280986825332164
2312	60-61	0.35802627194367176
2312	62-63	0.3990954770783688
2312	64-65	0.41070052222958253
2312	66-67	0.44221580073489974
2312	68-69	0.4312973996640821
2312	70-71	0.43639269466190456
2312	72-73	0.5006723589249518
2312	74-75	0.39866614765593056
2312	76-77	0.39495899389165245
2312	78-79	0.2895869035146319
2312	80-81	0.28433348579049067
2312	82-83	0.26217046820986667
2312	84-85	0.2796709174024308
2312	86-87	0.3037986688165013
2312	88-89	0.33071829929484053
2312	90-91	0.3295550272420442
2312	92-93	0.42351450731973017
2312	94-95	0.3221476834673318
2312	96-97	-1.052827963918947
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2313	1	0.1706584096476078
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2313	4	0.10900858689692683
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2313	14-15	0.1623007013740292
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2313	20-21	0.182952940699181
2313	22-23	0.2162918817832562
2313	24-25	-1.129510895163918
2313	26-27	-1.0788712714482713
2313	28-29	-1.161319354877179
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2313	32-33	0.2925012814899759
2313	34-35	0.24113233726370709
2313	36-37	0.15934597185881927
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2313	40-41	0.17549786132936163
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2313	56-57	-0.04627115953483241
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2313	60-61	-0.27693615826996876
2313	62-63	-2.021412055660093
2313	64-65	-0.5913384780008002
2313	66-67	-0.6082990458557518
2313	68-69	-0.6252362020544098
2313	70-71	-1.912060469138332
2313	72-73	-0.6906009707325751
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2313	76-77	-0.6396266998014184
2313	78-79	-0.662616096377004
2313	80-81	-0.48014522396197634
2313	82-83	-0.4270268252721223
2313	84-85	-0.25733269564396366
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2313	92-93	0.12249254288425249
2313	94-95	0.09281035455831699
2313	96-97	-0.09543253874458912
2313	98-99	0.1912389279703035
2313	100	0.2912797927802231
2314	1	-2.0077892380878417
2314	2	0.015934393290713444
2314	3	-0.009154636116150527
2314	4	-0.10825078366072205
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2314	8	0.07101043393475237
2314	9	0.07810013694655993
2314	10-11	-0.8727604644055802
2314	12-13	-1.182797391506341
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2314	36-37	0.274707340675441
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2314	64-65	0.43341326173013073
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2314	68-69	0.5220930081605673
2314	70-71	0.34235308873573445
2314	72-73	0.5132129092506403
2314	74-75	0.5230406375565337
2314	76-77	0.5629231259463658
2314	78-79	0.4626754792117822
2314	80-81	0.4117966676061755
2314	82-83	0.3367996557194388
2314	84-85	0.3513942301012598
2314	86-87	0.3315183916979869
2314	88-89	0.2770402708272819
2314	90-91	0.28583914550632983
2314	92-93	0.1081416744692838
2314	94-95	-0.03786174907771667
2314	96-97	0.07469234502152133
2314	98-99	0.16352024930266396
2314	100	0.1866680766776554
2315	1	0.06578371579481512
2315	2	0.013946367538501647
2315	3	0.006434870923435199
2315	4	-0.11590915558955572
2315	5	0.007253046629635662
2315	6	0.023087355051458758
2315	7	0.0010463290311690798
2315	8	0.003549095381984557
2315	9	-2.8649550075954267E-4
2315	10-11	0.06064267302665627
2315	12-13	0.06093334234488168
2315	14-15	0.042104362428510456
2315	16-17	0.15071431031217486
2315	18-19	0.1310229723690881
2315	20-21	0.1307668944901721
2315	22-23	0.16003295679173846
2315	24-25	0.28285310534091224
2315	26-27	0.14361867306442377
2315	28-29	-1.1696850743729144
2315	30-31	0.22059642549448455
2315	32-33	-1.3825532156701925
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2315	44-45	-0.7865596970626498
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2315	50-51	0.3580768179561815
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2315	54-55	0.3881421679226662
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2315	60-61	0.3920858742573401
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2315	64-65	0.4374832524458512
2315	66-67	0.4226349182326956
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2315	78-79	0.31871924816473296
2315	80-81	0.2571936587716124
2315	82-83	0.20117249857995745
2315	84-85	0.20356639400133147
2315	86-87	0.30925490634223607
2315	88-89	0.3104404351385135
2315	90-91	0.32262214041404036
2315	92-93	0.46283782071576596
2315	94-95	0.4018949293244667
2315	96-97	0.34641077129301934
2315	98-99	0.4470437739712807
2315	100	0.3280370926958902
2316	1	0.03939183082750475
2316	2	-0.1030189293055912
2316	3	-0.11029211217852009
2316	4	-0.02194626601832539
2316	5	-0.041148841813559045
2316	6	-0.052944927655275364
2316	7	0.015075166007534335
2316	8	0.060059605733840726
2316	9	-0.09207700517860218
2316	10-11	0.02574307615190463
2316	12-13	0.01655010014660263
2316	14-15	0.06302586376961017
2316	16-17	-1.2944639635070203
2316	18-19	0.08517712867287486
2316	20-21	0.0061825578097440825
2316	22-23	0.04694890520726602
2316	24-25	0.16485654093566637
2316	26-27	0.026304520883300597
2316	28-29	0.12827450515852945
2316	30-31	0.17712171484581773
2316	32-33	0.19776063757351636
2316	34-35	0.20864247452182383
2316	36-37	0.11636542221747703
2316	38-39	0.1356153853717359
2316	40-41	0.014452510492688475
2316	42-43	0.008109929886693124
2316	44-45	0.09817607970236253
2316	46-47	0.15181258298989064
2316	48-49	0.17316672309329562
2316	50-51	0.19812661425240918
2316	52-53	0.14641794765503136
2316	54-55	0.19805876888201368
2316	56-57	0.16409511604485516
2316	58-59	0.16633373700782528
2316	60-61	-1.200178855695242
2316	62-63	0.1519815589651401
2316	64-65	0.18498550334511066
2316	66-67	0.024507359820326258
2316	68-69	0.18756943116930813
2316	70-71	0.13548744383680855
2316	72-73	0.24975125402259835
2316	74-75	0.1779026685194367
2316	76-77	-0.03845404015293852
2316	78-79	-0.10198717534082391
2316	80-81	0.06966342216128396
2316	82-83	-0.020564280281043068
2316	84-85	-0.5963834410192987
2316	86-87	0.0486058073639235
2316	88-89	0.051994788060635955
2316	90-91	0.15807324799331113
2316	92-93	0.39682247526188164
2316	94-95	0.33816681299695617
2316	96-97	0.3330945950287756
2316	98-99	0.3697511903874222
2316	100	0.13151757557958632
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	1.0
11	15.0
12	70.0
13	491.0
14	1817.0
15	4866.0
16	10408.0
17	18928.0
18	30661.0
19	43754.0
20	58552.0
21	76618.0
22	96963.0
23	114001.0
24	128471.0
25	146847.0
26	171538.0
27	205011.0
28	247253.0
29	302136.0
30	370309.0
31	454828.0
32	567082.0
33	737358.0
34	1046282.0
35	1853838.0
36	2209327.0
37	1795696.0
38	1533634.0
39	260626.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.54026982775065	18.484656620116564	13.07871205835172	23.896361493781065
2	16.567044346515086	20.780966377216675	38.62640929176519	24.025579984503047
3	19.90718700137538	25.35210681102465	29.46401093896084	25.276695248639136
4	13.5078585349986	16.466807213198667	36.47134457161042	33.55398968019231
5	14.057895367371204	36.69811323776625	33.9105647409746	15.333426653887944
6	31.6363421457374	35.42210378175763	18.03316667566235	14.90838739684263
7	28.112359331015917	31.793336553627512	21.674026576395928	18.420277538960647
8	26.496230180227904	34.267141606860704	20.07294193450121	19.163686278410186
9	26.722592037221293	15.925165015345378	20.122314559188307	37.22992838824502
10-11	25.536290427363067	25.384998815012498	27.926565770089525	21.152144987534907
12-13	26.0751660688261	23.195068838352256	27.960155653963746	22.7696094388579
14-15	23.30531425240222	24.672029310955015	26.06921387865519	25.95344255798757
16-17	22.82122422189861	27.41390522452445	26.711501925100446	23.053368628476495
18-19	22.613026916265174	26.667992262322514	28.427336110090533	22.29164471132178
20-21	23.434529497178108	25.709933657735935	28.35232880213951	22.503208042946454
22-23	23.145961610172307	25.62013226696213	28.13540436551366	23.0985017573519
24-25	23.091252824752658	25.751509537889767	28.14555198257635	23.011685654781232
26-27	23.039380955552232	25.616360748738217	28.416578327990027	22.927679967719524
28-29	22.816539952161147	26.103325625557805	28.344449826970497	22.73568459531055
30-31	23.328723074404007	25.71738820442448	28.227862998625653	22.726025722545863
32-33	23.09674889165487	25.938819861301294	28.148394515287308	22.816036731756533
34-35	23.39900832795014	25.922100277854614	28.10081716198746	22.578074232207783
36-37	23.403913342244845	25.785247362914838	28.09574283593082	22.715096458909496
38-39	23.21117154860134	26.09231043485082	27.965565646177886	22.730952370369952
40-41	23.193930570104207	26.05219575168738	27.957121257259644	22.796752420948774
42-43	23.114382822689333	26.0933000000921	27.67952005001828	23.112797127200288
44-45	23.15408050336164	26.03761357467027	27.658718231667223	23.149587690300866
46-47	23.222238334031932	26.096005855675052	27.597816071558217	23.08393973873479
48-49	23.248123311781537	26.140692046926716	27.541088909668677	23.070095731623073
50-51	22.907202927751587	26.475682503495758	27.55300945245581	23.06410511629684
52-53	23.110164602367885	26.589287681469997	27.04876186322245	23.25178585293967
54-55	22.92232353804661	26.356083506192746	27.258139768428446	23.463453187332195
56-57	23.173747276623025	26.3034159979425	27.11205139382885	23.41078533160562
58-59	23.34637579929336	26.20158144187811	27.215992198146388	23.236050560682145
60-61	23.25061429055697	26.39875379279323	27.452403342983505	22.898228573666294
62-63	22.966314237010554	26.429289263189826	27.39805339862445	23.206343101175168
64-65	22.9315058324025	26.44904151857281	27.219628081362263	23.39982456766243
66-67	22.989684777499185	26.306776366841056	27.040786010184693	23.66275284547507
68-69	22.838691899593886	26.35499362500792	27.088164761042606	23.71814971435559
70-71	22.986334279616734	26.264385442984757	27.07321751038042	23.676062767018085
72-73	22.978735246268247	26.359161198020836	27.037713735660695	23.624389820050222
74-75	22.850471011406885	26.73399216508602	26.76337519474065	23.652161628766443
76-77	22.840258745183164	26.335645174174893	26.977514860189387	23.846581220452553
78-79	22.8332733177975	26.37215715856542	27.00848066970531	23.78608885393177
80-81	22.943235805948476	26.499677636785744	26.75295958740071	23.804126969865067
82-83	22.906778841884147	26.451829044212595	26.86197548899212	23.77941662491114
84-85	22.828379086657907	26.639165689350936	26.694489300645007	23.837965923346147
86-87	22.732671248662452	26.612245790285368	26.74149580546063	23.913587155591554
88-89	22.59400799907235	26.497820050982394	26.691230265783688	24.216941684161565
90-91	22.973475911462113	26.309192309301622	26.65735863425779	24.05997314497848
92-93	22.86950627457649	26.476893759409727	26.663125234911483	23.990474731102307
94-95	22.701346381289472	26.33628917556018	26.77619803462238	24.18616640852797
96-97	22.507566926740232	26.322882822107307	26.946167270323073	24.223382980829385
98-99	22.66289722021476	26.352694189108618	26.588026999809365	24.396381590867257
100	22.5961528451498	26.07956684873195	26.703744924308882	24.62053538180937
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3627.0
1	2815.0
2	4708.0
3	6635.0
4	4384.0
5	3191.0
6	4645.5
7	8481.0
8	12743.5
9	14717.0
10	13017.0
11	10687.5
12	10780.0
13	11637.0
14	12748.0
15	13753.5
16	14697.0
17	15906.0
18	17125.0
19	18396.0
20	20098.5
21	22260.0
22	25373.5
23	30048.0
24	35875.0
25	43066.0
26	52933.0
27	64551.0
28	77479.0
29	92769.0
30	111265.0
31	130146.0
32	149638.5
33	169856.5
34	190075.0
35	211255.0
36	238807.0
37	267185.5
38	288207.0
39	308265.0
40	323812.5
41	336533.5
42	352916.0
43	372185.0
44	391098.0
45	412492.5
46	436635.5
47	472874.0
48	528952.0
49	548477.5
50	538116.0
51	520395.5
52	490785.0
53	477740.5
54	467047.5
55	442793.0
56	406366.5
57	367500.0
58	332569.0
59	296025.0
60	254167.0
61	212642.5
62	175043.5
63	138593.0
64	105044.5
65	79746.5
66	60750.5
67	47314.0
68	37145.5
69	28770.0
70	22979.0
71	18220.5
72	14706.0
73	12184.5
74	9720.0
75	7324.0
76	5222.5
77	3435.5
78	2334.0
79	1615.0
80	1074.0
81	695.0
82	464.0
83	303.0
84	197.5
85	131.5
86	98.0
87	69.5
88	45.0
89	29.0
90	20.5
91	16.5
92	11.0
93	7.0
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.044140228508965984
2	0.009209227646101393
3	0.0
4	2.6426479332290954E-4
5	4.084092260444966E-4
6	8.008024040088168E-6
7	2.4024072120264505E-5
8	6.406419232070534E-5
9	0.0
10-11	1.2012036060132253E-5
12-13	2.5625676928282137E-4
14-15	0.009785805376987743
16-17	0.0016376409161980305
18-19	5.605616828061718E-5
20-21	8.888906684497867E-4
22-23	6.286298871469213E-4
24-25	0.0043323410056876985
26-27	0.015291321904548356
28-29	0.012800826428080938
30-31	0.009541560643765053
32-33	0.006734748217714149
34-35	0.008720738179656016
36-37	0.005041051133235502
38-39	0.0037517592627813066
40-41	0.005085095265455987
42-43	0.006730744205694106
44-45	0.006934948818716353
46-47	0.01449051950053954
48-49	0.016416449282180747
50-51	0.01593196382775541
52-53	0.010846868562299425
54-55	0.0035956027939995877
56-57	0.0036076148300597196
58-59	0.009701721124566815
60-61	0.0024504553562669793
62-63	1.481484447416311E-4
64-65	3.163169495834826E-4
66-67	8.408425242092577E-4
68-69	6.806820434074943E-5
70-71	2.002006010022042E-4
72-73	0.003043049135233504
74-75	0.005409420239079558
76-77	0.005385396166959293
78-79	0.0034474543492579563
80-81	6.406419232070534E-5
82-83	8.488505482493458E-4
84-85	3.2032096160352676E-4
86-87	3.923931779643202E-4
88-89	2.1621664908238057E-4
90-91	4.2042126210462883E-4
92-93	4.6846940634515783E-4
94-95	1.6016048080176336E-5
96-97	0.0019139177455810721
98-99	4.28429286144717E-4
100	1.0410431252114619E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2487475E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.744962643929505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.30130091992129	33.479007001493436
2	17.587321662422013	17.497613182079107
3	6.689234305660166	9.982671319546698
4	3.060124662620076	6.089031481112123
5	1.615443727077725	4.018009392842584
6	0.9400180988494501	2.805669912713012
7	0.6113530463000595	2.1288214115314275
8	0.42513116541719437	1.6918507153958837
9	0.28651795467210156	1.28275424567809
>10	1.373512805858141	11.84008649629216
>50	0.07301842301539567	2.4907811441166627
>100	0.03400490095602414	3.0857435804210716
>500	0.0019231873105211433	0.6576421267645871
>1k	8.031026375466157E-4	0.7472886765694371
>5k	1.4601864116034458E-4	0.44700759643907284
>10k+	1.4601864116034458E-4	1.7560217170046688
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37891	0.3034320389029808	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34349	0.2750676177529885	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	29610	0.23711759182701067	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21524	0.17236470943885773	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6016048080176336E-5	2.4024072120264505E-5
2	0.0	0.0	0.0	1.6016048080176336E-5	2.4024072120264505E-5
3	0.0	0.0	0.0	4.0040120200440844E-5	2.4024072120264505E-5
4	0.0	0.0	0.0	4.0040120200440844E-5	3.203209616035267E-5
5	0.0	0.0	0.0	4.0040120200440844E-5	3.203209616035267E-5
6	0.0	0.0	0.0	7.207221636079351E-5	4.0040120200440844E-5
7	0.0	0.0	0.0	7.207221636079351E-5	4.804814424052901E-5
8	0.0	0.0	0.0	7.207221636079351E-5	6.406419232070534E-5
9	0.0	0.0	0.0	8.808826444096985E-5	9.609628848105802E-5
10-11	4.004012020044084E-6	0.0	0.0	1.241243726213666E-4	9.609628848105802E-5
12-13	8.008024040088168E-6	0.0	0.0	1.561564687817193E-4	9.609628848105802E-5
14-15	8.008024040088168E-6	0.0	0.0	1.6816850484185154E-4	1.4414443272158705E-4
16-17	8.008024040088168E-6	0.0	0.0	1.9619658898216013E-4	2.2022066110242462E-4
18-19	8.008024040088168E-6	0.0	0.0	2.242246731224687E-4	2.8828886544317404E-4
20-21	8.008024040088168E-6	0.0	0.0	2.8428485342313E-4	2.9629688948326226E-4
22-23	8.008024040088168E-6	0.0	0.0	4.4044132220484925E-4	3.363370096837031E-4
24-25	1.6016048080176336E-5	0.0	0.0	7.887903679486846E-4	3.7237311786409984E-4
26-27	1.6016048080176336E-5	0.0	0.0	0.0014454483392359143	3.763771298841439E-4
28-29	1.6016048080176336E-5	0.0	0.0	0.0036876950704606013	3.80381141904188E-4
30-31	1.6016048080176336E-5	0.0	0.0	0.011219241680163524	3.8838916594427617E-4
32-33	1.6016048080176336E-5	8.008024040088168E-6	0.0	0.030082142306591203	4.084092260444966E-4
34-35	2.4024072120264505E-5	8.008024040088168E-6	0.0	0.06107719935375246	4.4044132220484925E-4
36-37	2.4024072120264505E-5	8.008024040088168E-6	0.0	0.10895717508943961	4.4044132220484925E-4
38-39	2.4024072120264505E-5	8.008024040088168E-6	0.0	0.18013249275774326	4.4444533422489333E-4
40-41	2.4024072120264505E-5	8.008024040088168E-6	0.0	0.26322775420971817	4.8448545442533416E-4
42-43	3.603610818039676E-5	8.008024040088168E-6	0.0	0.3427994850840542	5.125135385656427E-4
44-45	4.0040120200440844E-5	8.008024040088168E-6	0.0	0.44306395007797816	5.405416227059513E-4
46-47	4.0040120200440844E-5	8.008024040088168E-6	0.0	0.5674886236008481	5.765777308863482E-4
48-49	4.0040120200440844E-5	1.2012036060132253E-5	0.0	0.6947961857781497	6.246258751268772E-4
50-51	4.0040120200440844E-5	1.6016048080176336E-5	0.0	0.8269566105237448	6.246258751268772E-4
52-53	4.0040120200440844E-5	1.6016048080176336E-5	0.0	0.9601420623464711	6.966980914876707E-4
54-55	4.0040120200440844E-5	1.6016048080176336E-5	0.0	1.1151053355462173	7.727743198685083E-4
56-57	4.0040120200440844E-5	2.0020060100220422E-5	0.0	1.2985251221724168	7.807823439085964E-4
58-59	4.0040120200440844E-5	2.4024072120264505E-5	0.0	1.4994064052180285	8.08810428048905E-4
60-61	4.404413222048493E-5	2.4024072120264505E-5	0.0	1.705264675204555	8.368385121892136E-4
62-63	4.804814424052901E-5	2.4024072120264505E-5	0.0	1.9184102470675617	8.808826444096985E-4
64-65	4.804814424052901E-5	2.4024072120264505E-5	0.0	2.1394677466821754	8.848866564297426E-4
66-67	4.804814424052901E-5	2.4024072120264505E-5	0.0	2.3930137998274272	8.888906684497867E-4
68-69	5.2052156260573094E-5	2.4024072120264505E-5	0.0	2.681282645210501	9.04906716529963E-4
70-71	5.605616828061718E-5	2.4024072120264505E-5	0.0	2.9925665516847886	9.209227646101393E-4
72-73	5.605616828061718E-5	3.203209616035267E-5	0.0	3.292835421091934	0.0011051073175321671
74-75	5.605616828061718E-5	3.203209616035267E-5	0.0	3.599370569310449	0.0011051073175321671
76-77	5.605616828061718E-5	3.203209616035267E-5	0.0	3.938854732441907	0.0011051073175321671
78-79	5.605616828061718E-5	3.203209616035267E-5	0.0	4.322282927493348	0.0011051073175321671
80-81	5.605616828061718E-5	3.203209616035267E-5	0.0	4.726415868700437	0.0011331354016724756
82-83	5.605616828061718E-5	3.203209616035267E-5	0.0	5.135061331454117	0.0011811835459130046
84-85	5.605616828061718E-5	3.203209616035267E-5	0.0	5.549128226482936	0.0012012036060132254
86-87	5.605616828061718E-5	3.203209616035267E-5	0.0	5.979919879719478	0.0012012036060132254
88	5.605616828061718E-5	3.203209616035267E-5	0.0	6.324945595486677	0.001233235702173578
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	17085	0.0	46.13135	2
GTACCTG	19845	0.0	40.325165	1
ACCTGGG	20635	0.0	37.689873	3
CCTGGGG	19275	0.0	26.988848	4
ATAACGC	1370	0.0	20.923792	3
GTACATG	55865	0.0	20.603418	1
CATGGGG	30485	0.0	20.471188	4
TATAACG	1250	0.0	20.303078	2
TACATGG	57260	0.0	19.583809	2
GAGTACT	39575	0.0	19.347477	12-13
ACATGGG	56905	0.0	19.051462	3
ATGGGGG	16845	0.0	18.495749	5
TAACGCA	1385	0.0	18.322094	4
GTATAGG	3460	0.0	18.07354	1
AGTACTT	41420	0.0	17.498405	12-13
CTGGGGG	12180	0.0	17.323214	5
GTATCAA	83615	0.0	17.162006	1
GTACTTT	44600	0.0	17.01876	14-15
GTACACG	2830	0.0	16.281984	1
GGTATCA	68360	0.0	16.252844	1
>>END_MODULE