FastQCFastQC Report
Mon 6 Jun 2016
ERR523004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3880228
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT356120.9177811200785109No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140360.36173132094299615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113460.292405497821262No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT96770.24939256146803745No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC79410.20465292245713396No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC66990.17264449408642998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65160.16792827637963542No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA58000.14947575245578354No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG53350.13749192057786294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG33800.035.3371732
GTACCTG41400.029.4195941
ACCTGGG44650.026.7519323
GTACATG128550.025.8999211
TATCACG3700.025.4180982
TACATGG132650.024.920852
ACATGGG133400.023.901043
CATGGGG89100.022.3756074
ATGGGGG58350.020.145345
GAGTACT124500.020.13132912-13
AGAGTAC180550.019.30608210-11
TAACGCA3055.18412E-918.4998444
AGTACTT132050.017.57502612-13
GTACTTT142750.017.41065214-15
TATAACG3101.254648E-716.6857532
CCTGGGG42150.016.6216964
TGGGGGG61700.016.4477656
GAGTACA101150.015.8070321
GTGTAGC18550.015.7175581
ATAACGC3654.749927E-815.4607763
Produced by FastQC (version 0.11.5)