##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523004_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3880228 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84280923698298 34.0 31.0 34.0 30.0 34.0 2 32.14668777195567 34.0 31.0 34.0 29.0 35.0 3 31.986583004916206 34.0 31.0 34.0 28.0 35.0 4 34.599382819772444 35.0 35.0 37.0 32.0 37.0 5 34.614656922222096 37.0 34.0 37.0 32.0 37.0 6 34.78816863338958 37.0 35.0 37.0 32.0 37.0 7 34.64278645481657 37.0 35.0 37.0 32.0 37.0 8 34.780472951589445 37.0 35.0 37.0 32.0 37.0 9 36.02607295241414 39.0 35.0 39.0 32.0 39.0 10-11 36.12368280936069 38.0 35.5 39.0 32.0 39.0 12-13 36.172430460271926 38.0 35.5 39.0 32.5 39.0 14-15 37.247001722579185 39.0 36.5 41.0 32.0 41.0 16-17 37.1416503102395 39.0 36.5 41.0 32.0 41.0 18-19 37.17487052307236 39.0 36.5 41.0 32.0 41.0 20-21 37.195361586999525 39.0 36.0 41.0 32.0 41.0 22-23 37.094919937694385 39.0 36.0 41.0 32.0 41.0 24-25 37.087554391133715 39.0 36.5 41.0 32.0 41.0 26-27 36.907628495026586 39.0 36.5 41.0 32.0 41.0 28-29 36.711189265166894 39.0 35.0 41.0 31.0 41.0 30-31 36.62179580684434 38.0 36.0 41.0 30.5 41.0 32-33 36.43550159939055 38.0 35.0 41.0 30.0 41.0 34-35 36.309686054530815 38.5 35.0 41.0 30.0 41.0 36-37 36.18105224744525 38.0 35.5 40.5 30.0 41.0 38-39 35.93457278799081 38.0 35.5 40.0 29.0 41.0 40-41 35.89429925251815 38.0 35.0 40.0 29.5 41.0 42-43 35.83842444309974 38.0 35.0 40.0 29.0 41.0 44-45 35.72418721271018 38.0 35.0 40.0 28.0 41.0 46-47 35.432427682084665 38.0 34.5 40.0 27.0 41.0 48-49 35.52414059173842 37.5 35.0 40.0 26.5 41.0 50-51 34.51989135689965 37.5 33.5 39.0 25.0 40.0 52-53 34.69110230120498 37.0 34.5 39.5 26.0 40.0 54-55 35.267954486179676 37.0 34.5 40.0 27.0 41.0 56-57 35.40108764227257 37.0 35.0 40.0 26.5 41.0 58-59 35.38556432766322 38.0 35.0 40.0 26.5 41.0 60-61 35.115347989860396 37.0 35.0 40.0 26.0 41.0 62-63 34.91822890304384 37.0 34.0 40.0 26.0 41.0 64-65 34.69220197885279 36.5 34.0 39.5 26.0 41.0 66-67 34.445138661954914 37.0 34.0 39.0 25.0 41.0 68-69 34.10306030985808 36.0 34.0 39.0 25.0 41.0 70-71 33.68947301550321 36.0 33.5 38.0 24.0 40.5 72-73 33.43448271596411 35.5 33.5 37.5 23.5 40.0 74-75 33.17516779941798 35.0 33.0 37.0 22.5 39.0 76-77 32.83711073163742 35.0 33.0 37.0 22.5 39.0 78-79 32.52650024689271 35.0 32.5 37.0 21.5 38.5 80-81 32.292167496343 35.0 32.0 36.0 20.5 38.5 82-83 32.05855326027233 35.0 32.0 36.0 20.0 38.0 84-85 31.763018564888455 35.0 32.0 36.0 19.5 37.0 86-87 31.673492768981617 35.0 32.0 36.0 19.5 37.0 88-89 31.35824789161874 35.0 31.0 35.5 19.0 37.0 90-91 31.25254057235812 35.0 31.0 35.5 18.0 36.5 92-93 31.01117137961996 35.0 31.0 35.0 18.0 36.0 94-95 30.91038258576558 34.5 31.0 35.0 18.5 36.0 96-97 30.649626259075497 34.0 30.0 35.0 18.0 36.0 98-99 30.59828056495649 34.5 30.0 35.0 18.0 36.5 100 29.377640179906955 34.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10073899271420217 1101 2 0.1611918990665373 1101 3 0.06570571387744906 1101 4 0.06565462012401468 1101 5 0.10228047881618352 1101 6 0.05148093073270843 1101 7 0.2220319664840602 1101 8 0.13127721587218133 1101 9 0.04606389293024904 1101 10-11 0.06602054725112083 1101 12-13 0.0380245120514644 1101 14-15 0.16038215234971176 1101 16-17 -0.01711828620191369 1101 18-19 0.06783942440608826 1101 20-21 0.007308463731476422 1101 22-23 -0.048041877888593376 1101 24-25 -0.11941658318117732 1101 26-27 -0.1297268409966108 1101 28-29 -0.047536606296468165 1101 30-31 -0.12116554837178484 1101 32-33 -0.15044117832863435 1101 34-35 -0.19015111894537284 1101 36-37 -0.06231154866049593 1101 38-39 -0.20563422928442066 1101 40-41 -0.21632152619663714 1101 42-43 -0.1100340673737179 1101 44-45 -0.21580850888265246 1101 46-47 -0.25659270451730976 1101 48-49 -0.3247152803409321 1101 50-51 -0.45414406945224073 1101 52-53 -0.30279868256473463 1101 54-55 -0.2764739950539834 1101 56-57 -0.1474783623253373 1101 58-59 -0.11336451701609462 1101 60-61 -0.06748516285531281 1101 62-63 -0.24741335460839764 1101 64-65 -0.1937114832450746 1101 66-67 -0.37675170093743304 1101 68-69 -0.21316754104135072 1101 70-71 -0.36752550771457493 1101 72-73 -0.21577309746789552 1101 74-75 -0.36489616864880503 1101 76-77 -0.31262417813101706 1101 78-79 -0.5371454819336137 1101 80-81 -0.23159494333452812 1101 82-83 -0.2535359832330073 1101 84-85 -0.5197403254023492 1101 86-87 -0.8280212558891442 1101 88-89 -0.348766230639729 1101 90-91 -0.6127096294613743 1101 92-93 -0.35657003434812395 1101 94-95 -0.5515777176834398 1101 96-97 -0.5371339350333599 1101 98-99 -0.4530262075849336 1101 100 -0.5874715017531855 1102 1 0.00898970056939774 1102 2 0.012529233288958608 1102 3 0.04343104717591473 1102 4 -0.007507317096603572 1102 5 0.08076422320672805 1102 6 0.10616497816808845 1102 7 0.15790064899447742 1102 8 0.01598209582372334 1102 9 -0.03541833914165693 1102 10-11 0.003386766614326575 1102 12-13 -0.04653738042427591 1102 14-15 -0.049113567105735 1102 16-17 -0.09219353894508231 1102 18-19 -0.11396913290584365 1102 20-21 -0.0440507344636174 1102 22-23 -0.09902996965045929 1102 24-25 -0.03528437951065655 1102 26-27 -0.14011923756403633 1102 28-29 -0.4724579758963898 1102 30-31 -0.1851786841884575 1102 32-33 -0.1782711100345864 1102 34-35 -0.08991272174163356 1102 36-37 -0.22256589712137043 1102 38-39 -0.2970965658675695 1102 40-41 -0.6276304663096965 1102 42-43 -0.17341928587543265 1102 44-45 -0.28058390559390745 1102 46-47 -0.3545944027886705 1102 48-49 -0.5914322123534177 1102 50-51 -0.42537006448091574 1102 52-53 -0.5774401424679496 1102 54-55 -0.3978899378851466 1102 56-57 -0.1962047314385913 1102 58-59 -0.18271257112252215 1102 60-61 -0.622183592571723 1102 62-63 -0.3230540924920504 1102 64-65 -0.27419465524989306 1102 66-67 -0.36664201062135504 1102 68-69 -0.5025226192214021 1102 70-71 -0.1779386786879371 1102 72-73 -0.5218960267462975 1102 74-75 -0.3331880693907223 1102 76-77 -0.5690718346182209 1102 78-79 -0.35661531328320706 1102 80-81 -0.2257573715865604 1102 82-83 -0.36231741904953196 1102 84-85 -0.32115875970946206 1102 86-87 -0.3933039911956122 1102 88-89 -0.3294860928603818 1102 90-91 -0.2687004306125367 1102 92-93 -0.3905633545776013 1102 94-95 -0.574123286996592 1102 96-97 -0.49265251795737797 1102 98-99 -0.5067058560761737 1102 100 -0.5162991860461368 1103 1 0.06747498003842622 1103 2 -0.010203426740552857 1103 3 -0.06920218867751515 1103 4 0.1491909639544744 1103 5 0.04790337443908044 1103 6 0.05337734586441911 1103 7 0.17052176644444472 1103 8 0.07869749711599638 1103 9 0.09612826211226633 1103 10-11 0.05449846367022104 1103 12-13 0.1370837316989153 1103 14-15 0.05121449538793854 1103 16-17 0.10826050167687384 1103 18-19 -0.12385695111381523 1103 20-21 -0.044612708777926 1103 22-23 -0.10296612031284269 1103 24-25 -0.08210969072723628 1103 26-27 -0.1963662745478132 1103 28-29 -0.24635320364247093 1103 30-31 -0.1388670734262547 1103 32-33 -0.22431471764335242 1103 34-35 -0.20659496921275178 1103 36-37 -0.16518092873575796 1103 38-39 -0.07751975116994458 1103 40-41 -0.050432241189703575 1103 42-43 -0.10828619650820315 1103 44-45 -0.19040001215063285 1103 46-47 -0.2356306328199409 1103 48-49 -0.2412036938881741 1103 50-51 -0.23965852776081675 1103 52-53 -0.16659596915613406 1103 54-55 -0.0859362648103783 1103 56-57 -0.024902109602024325 1103 58-59 0.04248565133407567 1103 60-61 -0.11551447559050843 1103 62-63 -0.028798282316856216 1103 64-65 -0.043424297663769096 1103 66-67 -0.046003037835824045 1103 68-69 -0.0652842968345837 1103 70-71 -0.06608215259475259 1103 72-73 -0.23368010875726242 1103 74-75 -0.23184259368346005 1103 76-77 -0.2323137206146768 1103 78-79 -0.2006704373703272 1103 80-81 -0.2600065099813875 1103 82-83 -0.2057666229636439 1103 84-85 -0.22378072944275118 1103 86-87 -0.3728395367368371 1103 88-89 -0.23149548395721808 1103 90-91 -0.26124894417715794 1103 92-93 -0.33579116724572344 1103 94-95 -0.4358939189114075 1103 96-97 -0.4490991756750695 1103 98-99 -0.4062496181612829 1103 100 -0.31432335360504027 1104 1 0.05594093755330931 1104 2 0.08252944003024254 1104 3 0.08777792423992992 1104 4 -0.0839751893188776 1104 5 0.03882867498504794 1104 6 0.0020117632866316626 1104 7 0.11404770226010186 1104 8 -0.018956964460947745 1104 9 -0.04483841531675381 1104 10-11 -0.19244939127653282 1104 12-13 -0.09992501780295981 1104 14-15 -0.07729624951230818 1104 16-17 -0.10448557806343928 1104 18-19 -0.21968886643445984 1104 20-21 -0.27468214049661555 1104 22-23 -0.18509948767848527 1104 24-25 -0.09308521800979008 1104 26-27 -0.014217927150696141 1104 28-29 -0.2762090608347805 1104 30-31 -0.20422924113861285 1104 32-33 -0.16151940741126225 1104 34-35 -0.22396518832843526 1104 36-37 -0.2034715980668338 1104 38-39 -0.3481367103108184 1104 40-41 -0.2260491130682567 1104 42-43 -0.23065996429153302 1104 44-45 -0.2233204856359734 1104 46-47 -0.3018407357603863 1104 48-49 -0.3084476007967325 1104 50-51 -0.25971798975953675 1104 52-53 -0.1308348163045494 1104 54-55 -0.24471114547915107 1104 56-57 -0.6474430210860334 1104 58-59 -0.04631986568062274 1104 60-61 -0.16328419614747247 1104 62-63 -0.11068113410762237 1104 64-65 -0.14366150573495418 1104 66-67 -0.25123550465432487 1104 68-69 -0.1747226983634178 1104 70-71 -0.18573213616034678 1104 72-73 -0.12329185688330568 1104 74-75 0.021863182500325706 1104 76-77 -0.25981062280234113 1104 78-79 -0.06985647627187319 1104 80-81 -0.053992899968328345 1104 82-83 -0.06690693059240971 1104 84-85 -0.27882427334006366 1104 86-87 -0.17296014053391318 1104 88-89 -0.2342354480633162 1104 90-91 -0.4072899254710265 1104 92-93 -0.6303468347879218 1104 94-95 -0.4603421665385987 1104 96-97 -0.4175266868664629 1104 98-99 -0.2811315958744842 1104 100 -0.29452011559124713 1105 1 -0.007116646456314157 1105 2 -0.1271788996848855 1105 3 -0.008862970033796103 1105 4 0.06873251109117717 1105 5 0.03165715750081688 1105 6 -0.009336452576022225 1105 7 0.09081992592945909 1105 8 0.03126874683257341 1105 9 0.07495420676865905 1105 10-11 -0.03453780443179255 1105 12-13 -0.06187204467900642 1105 14-15 -0.012537488563012289 1105 16-17 -0.051280577198120625 1105 18-19 -0.07799441303674115 1105 20-21 0.013274297205960295 1105 22-23 -0.041508618760190075 1105 24-25 -0.010186539868094258 1105 26-27 -0.13886311994257028 1105 28-29 -0.15839270521484394 1105 30-31 -0.01742586245217126 1105 32-33 0.008978813444301181 1105 34-35 -0.08199422393726508 1105 36-37 -0.11612866579940828 1105 38-39 0.04483580241398499 1105 40-41 -0.16685689048632923 1105 42-43 -0.16998337412233866 1105 44-45 -0.18971055839023876 1105 46-47 -0.0587601737262986 1105 48-49 -0.13764145451278154 1105 50-51 -0.32126483795709504 1105 52-53 -0.22462037298504356 1105 54-55 -0.24510951178638862 1105 56-57 -0.1103875984179652 1105 58-59 -0.20807318205347514 1105 60-61 -0.05814502202728988 1105 62-63 -0.024280172911204545 1105 64-65 -0.12682663798653238 1105 66-67 -0.13134373520075115 1105 68-69 0.030675467161515257 1105 70-71 -0.08265060752825804 1105 72-73 -0.16265528102828597 1105 74-75 -0.10615067538773104 1105 76-77 -0.059157316273150684 1105 78-79 -0.00254582553464644 1105 80-81 0.061246903400046904 1105 82-83 -0.08913680110025979 1105 84-85 -0.09980844067807837 1105 86-87 -0.10659640046774754 1105 88-89 -0.0335624897439466 1105 90-91 -0.09511338487551058 1105 92-93 -0.15100708518655637 1105 94-95 -0.024347940540170754 1105 96-97 -0.06905858477808025 1105 98-99 -0.08892344183856693 1105 100 -0.14463146981280772 1106 1 -0.023336940819568497 1106 2 0.013610939253815957 1106 3 -0.016693703165998386 1106 4 0.01604966499331084 1106 5 0.037823615306052716 1106 6 -0.05521733187315192 1106 7 0.10254767647465002 1106 8 -0.08409166480532804 1106 9 -0.0824151986673698 1106 10-11 -0.06143980481790834 1106 12-13 -0.06385575234457974 1106 14-15 -0.07833740882630025 1106 16-17 -0.09862117803363901 1106 18-19 -0.0999169387712513 1106 20-21 -0.08066590568855503 1106 22-23 -0.06315083797673537 1106 24-25 -0.12351162346027422 1106 26-27 -0.13900637899206458 1106 28-29 -0.14554947609518365 1106 30-31 -0.13184782557451058 1106 32-33 -0.17738025477856922 1106 34-35 -0.14226462334050183 1106 36-37 -0.08067353254885745 1106 38-39 -0.02904831204589442 1106 40-41 0.02904637906144103 1106 42-43 -0.06255939959009993 1106 44-45 -0.08629733746129631 1106 46-47 -0.23317819142373963 1106 48-49 -0.1441664347785192 1106 50-51 -0.18914986459121508 1106 52-53 -0.1445935403830063 1106 54-55 -0.1578807793830137 1106 56-57 -0.2420914650771664 1106 58-59 -0.19949577404484842 1106 60-61 -0.09655122743955502 1106 62-63 -0.1307956501719616 1106 64-65 -0.041578757349412854 1106 66-67 -0.16200909691181664 1106 68-69 -0.18557694178955586 1106 70-71 -0.12247690887590323 1106 72-73 -0.12865437170994198 1106 74-75 -0.06626232962742762 1106 76-77 -0.091590979443815 1106 78-79 -0.11412873914597554 1106 80-81 -0.09159686548458268 1106 82-83 -0.16507619920234262 1106 84-85 -0.04507655821775458 1106 86-87 -0.21460636984108206 1106 88-89 -0.1610791796195521 1106 90-91 -0.24101228489732662 1106 92-93 -0.28108562384572267 1106 94-95 -0.1589074209264254 1106 96-97 -0.10208295502372167 1106 98-99 -0.058227057921165226 1106 100 -0.12519871710284747 1107 1 -0.09129776712264359 1107 2 -0.02379533014546098 1107 3 -9.079962173217382E-4 1107 4 -4.1292315476937347E-4 1107 5 0.03192623552015306 1107 6 -0.016326110729224297 1107 7 0.15893564858881604 1107 8 0.025595189017359132 1107 9 -0.022595369090709028 1107 10-11 0.024872177961114517 1107 12-13 0.07618357096402661 1107 14-15 0.0234683148252941 1107 16-17 0.04782381399451907 1107 18-19 -0.01017923930762521 1107 20-21 0.026965043608718986 1107 22-23 -0.1451645553580363 1107 24-25 -0.043764132247162024 1107 26-27 -0.0679468324271042 1107 28-29 0.08038495491150144 1107 30-31 0.0128018262992029 1107 32-33 -0.029460359551400472 1107 34-35 -0.00562477884732715 1107 36-37 -0.02781706597478717 1107 38-39 0.013181259260825584 1107 40-41 0.006534845564061698 1107 42-43 0.08627840236869133 1107 44-45 0.03386554388101359 1107 46-47 0.017122008877272776 1107 48-49 0.038730912628729186 1107 50-51 -0.12760559293212737 1107 52-53 -0.08648824390624554 1107 54-55 0.06574737083604276 1107 56-57 -0.049519740791396316 1107 58-59 0.04142480671642801 1107 60-61 0.0033885371118529406 1107 62-63 -0.03838810795690506 1107 64-65 0.04879451262598167 1107 66-67 0.013027077852491686 1107 68-69 0.0794701970545475 1107 70-71 0.004346623031587171 1107 72-73 0.10990815034103463 1107 74-75 -0.01799023287556878 1107 76-77 0.010233445667722663 1107 78-79 0.04933848919717576 1107 80-81 0.14082669299571648 1107 82-83 0.057795100141596834 1107 84-85 -0.010740119511648771 1107 86-87 0.10580825548214179 1107 88-89 0.09017493470163274 1107 90-91 0.08939059479255107 1107 92-93 4.753228165981227E-4 1107 94-95 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46-47 -0.1185573457944713 2316 48-49 -0.2721723342938418 2316 50-51 -0.18085087041021808 2316 52-53 -0.20575619960561653 2316 54-55 -0.18770419394207494 2316 56-57 -0.23825647630806657 2316 58-59 -0.3760381750073236 2316 60-61 -0.27799271737224984 2316 62-63 -0.20881219575281307 2316 64-65 -0.2705775258996468 2316 66-67 -0.2559418269427809 2316 68-69 -0.30108561358591857 2316 70-71 -0.1849414641868279 2316 72-73 -0.20546453198069514 2316 74-75 -0.12067448452274476 2316 76-77 -1.5242188537549204 2316 78-79 -0.08581615445849167 2316 80-81 -1.1120533247221793 2316 82-83 -0.19131090228724545 2316 84-85 -1.653513508192134 2316 86-87 -0.1001764824741791 2316 88-89 0.05361955571394006 2316 90-91 -0.818476399681412 2316 92-93 -0.0222364141610214 2316 94-95 0.024860000138250626 2316 96-97 0.16889314867326277 2316 98-99 0.10887420704915485 2316 100 -0.08491063786067343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 8879.0 1 703.0 2 490.0 3 440.0 4 360.0 5 379.0 6 460.0 7 1070.0 8 1680.0 9 1744.0 10 2509.0 11 4589.0 12 7378.0 13 9746.0 14 10970.0 15 12042.0 16 13606.0 17 15945.0 18 19432.0 19 22811.0 20 27193.0 21 32193.0 22 37066.0 23 40356.0 24 40971.0 25 41780.0 26 42820.0 27 46688.0 28 52945.0 29 62210.0 30 74979.0 31 93501.0 32 121155.0 33 167693.0 34 261772.0 35 474326.0 36 589748.0 37 627192.0 38 714092.0 39 196004.0 40 311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.98474868961086 18.1900631739656 12.853679819581165 22.97150831684237 2 15.794474346730366 21.435304560526763 39.06150799951771 23.708713093225164 3 20.287302461450672 26.037438717232327 29.9757546190516 23.699504202265402 4 12.70279512086359 16.61113257980794 38.68216861773149 32.00390368159698 5 12.949266591424049 37.71670300748865 34.4590925823075 14.874937818779802 6 29.60837519093451 36.799533045726314 18.55290188144004 15.039189881899137 7 26.372795325030307 32.790924535653446 22.956930740299196 17.87934939901705 8 25.443371664387794 33.07318266155103 21.170313507023913 20.313132167037256 9 26.209380494363426 16.741777846726652 21.33328162167753 35.71556003723239 10-11 24.833638088916253 25.31327271307947 29.033643051857045 20.819446146147232 12-13 26.2642305836016 23.71055188774723 28.19279634344542 21.83242118520575 14-15 22.981772558199104 25.243566528669863 27.4113910235177 24.363269889613328 16-17 21.998968464771366 27.850611160714923 27.622386252951515 22.5280341215622 18-19 21.60826598853724 27.963932600870482 28.85841764601484 21.56938376457744 20-21 23.060423530518907 26.197171956069106 28.463017186028168 22.279387327383827 22-23 22.327342902253665 26.216631411729196 28.726278855891646 22.72974683012549 24-25 22.90659748258495 26.154985045116707 28.624948279332063 22.313469192966274 26-27 22.39899693579965 25.65988616911884 29.70083592129963 22.240280973781882 28-29 21.826659647722067 27.63079851526569 28.680589239709715 21.861952597302533 30-31 22.757570156515175 26.185664817885822 28.619114524024024 22.437650501574975 32-33 22.698329626519374 26.41788380582663 28.768771198604874 22.115015369049125 34-35 23.253658353102246 26.268494700362815 28.58315410652891 21.894692840006034 36-37 22.875952851765486 26.01551450602047 29.19346880377959 21.91506383843445 38-39 22.407474583515075 26.991711023725674 28.76306310755295 21.837751285206302 40-41 22.59264532318906 26.889194668876353 28.430581843625735 22.087578164308855 42-43 22.087292983659488 27.11416694093469 27.67442347482633 23.12411660057949 44-45 22.490463554057914 26.40121743898103 28.08439869368818 23.023920313272882 46-47 23.025948422682106 26.783769865767436 27.911138272669646 22.279143438880805 48-49 22.66858339243913 26.81673721007023 28.011829261260722 22.502850136229917 50-51 21.744854588125303 27.18128154193133 28.77936297030589 22.29450089963748 52-53 22.545409967330066 27.596835630298 27.33820727097424 22.51954713139769 54-55 21.998013218999027 26.670853855472416 28.02294858783889 23.308184337689664 56-57 22.77400526518007 26.541543310483583 27.70335131802145 22.98110010631489 58-59 23.490694375429847 26.64466474809316 27.47225807102291 22.392382805454087 60-61 22.721547985278654 26.899106470140776 28.62408605686964 21.755259487710926 62-63 22.25178344701007 27.071050428920802 28.233037162187934 22.444128961881194 64-65 22.010872095007866 27.68727611635381 27.662540949437336 22.639310839200984 66-67 22.45439339393615 26.750721465182597 27.327635044759152 23.467250096122097 68-69 21.93533430383467 26.970537280969705 27.791377639964693 23.302750775230933 70-71 22.74008614368506 26.716336495763038 27.584394610808022 22.959182749743878 72-73 22.31369976166264 26.485010896475142 28.312755413416184 22.888533928446027 74-75 21.820786656119886 27.973598901251663 27.399836263634946 22.80577817899351 76-77 22.12986511941153 26.628750692839887 27.435838856981942 23.80554533076664 78-79 22.23353799567624 26.833443006970537 27.768007219451967 23.165011777901253 80-81 22.719989943656802 26.61626683347919 27.506271312017834 23.157471910846173 82-83 22.44136831451089 26.434729998983457 28.19831708975668 22.925584596748973 84-85 22.13730221019203 27.16657569017662 27.731961922556316 22.964160177075037 86-87 21.997414661322185 27.348607936337977 27.45176543250109 23.20221196983875 88-89 21.642433807085794 27.05921981161535 27.15537110896608 24.142975272332777 90-91 22.381589808601557 26.29992795546507 27.39561010702965 23.922872128903723 92-93 22.751349841742694 26.822751815304414 27.489746891743728 22.93615145120917 94-95 22.255504019205905 26.549868854316166 27.634080652073774 23.560546474404156 96-97 21.311252573746238 26.538194911483824 28.489818828958395 23.660733685811543 98-99 21.89776358736452 26.91274753779676 27.277773201198507 23.91171567364021 100 21.72012578209584 25.689479654836546 27.586766233379855 25.003628329687754 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6124.0 1 4725.0 2 3002.5 3 2275.5 4 1501.5 5 1194.0 6 1623.0 7 2651.5 8 4127.0 9 5011.0 10 4578.5 11 3966.0 12 4046.5 13 4329.5 14 4612.0 15 4907.0 16 5242.5 17 5563.0 18 5929.5 19 6472.0 20 7078.5 21 7951.5 22 9176.5 23 10787.0 24 13152.5 25 16299.5 26 20212.5 27 24898.5 28 30228.0 29 35626.0 30 41658.0 31 48441.0 32 55397.0 33 62186.0 34 68798.5 35 74879.0 36 81788.0 37 89687.5 38 96216.5 39 101529.5 40 105776.5 41 110414.0 42 114621.0 43 118751.0 44 123344.5 45 128956.0 46 136757.5 47 161806.5 48 211626.5 49 211800.5 50 167801.0 51 147552.0 52 139558.5 53 133504.5 54 127316.0 55 118505.5 56 108085.0 57 97682.5 58 86506.0 59 74411.0 60 62446.5 61 51650.0 62 41614.5 63 31825.0 64 23292.5 65 17266.5 66 12774.0 67 9778.5 68 7510.5 69 5571.0 70 4250.0 71 3301.0 72 2491.5 73 1857.0 74 1405.0 75 1058.0 76 766.5 77 503.0 78 329.0 79 220.5 80 153.5 81 104.5 82 79.0 83 74.5 84 59.5 85 46.5 86 44.5 87 30.0 88 23.5 89 22.5 90 17.0 91 13.5 92 11.5 93 12.0 94 9.5 95 8.5 96 6.0 97 2.5 98 4.5 99 4.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2037251419246498 2 0.18187075604835592 3 0.36948859706182213 4 0.3642569457258697 5 0.37397286963549564 6 0.3029461155375406 7 0.32165635627597144 8 0.3114507704186455 9 0.32544479345028177 10-11 0.29776600756450394 12-13 0.30634797748998255 14-15 0.29427394472695934 16-17 0.2896092703830806 18-19 0.312778012013727 20-21 0.3167210792767848 22-23 0.3086287712990062 24-25 0.3116698297110376 26-27 0.3010647827911143 28-29 0.3247231863694608 30-31 0.3070180412078878 32-33 0.306721666871122 34-35 0.30904111820233243 36-37 0.3169787960913637 38-39 0.32371809079260294 40-41 0.350842785527036 42-43 0.31846066777519255 44-45 0.30357752173325897 46-47 0.3096725243980508 48-49 0.33165576868163416 50-51 0.32366654742968715 52-53 0.3524535156181544 54-55 0.3523504288923228 56-57 0.35780113952066733 58-59 0.3425700757790522 60-61 0.3259086837165239 62-63 0.34762132534479934 64-65 0.3418355828575022 66-67 0.3286147102696027 68-69 0.33901358373786283 70-71 0.3581490572203489 72-73 0.36259467227183556 74-75 0.35169325101514654 76-77 0.33483857134168404 78-79 0.3466935448123151 80-81 0.3610999147472777 82-83 0.36562284484313806 84-85 0.3567960439438095 86-87 0.35590692093351217 88-89 0.34548227578379415 90-91 0.3574403359802568 92-93 0.33781520055007075 94-95 0.3586644908495068 96-97 0.36771035104122746 98-99 0.358857778460441 100 0.3817559174357795 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3880228.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.42019937541228 #Duplication Level Percentage of deduplicated Percentage of total 1 79.70929886053763 52.145982235306946 2 12.902303353170694 16.881425155329545 3 3.5434978386100893 6.954490052646436 4 1.3955278886855478 3.6518285084702673 5 0.7013843488732638 2.294235197104132 6 0.4338233990571083 1.702848795602104 7 0.26494617070224463 1.2132981917765 8 0.20216470645070903 1.0580524322141664 9 0.13481387119244098 0.7937595298782576 >10 0.678234841993603 7.517389836393258 >50 0.02425593425466899 1.0575330278633968 >100 0.008340620712509026 0.9831689347876036 >500 5.720673423140698E-4 0.2593247952604988 >1k 4.40051798532707E-4 0.5225647931314508 >5k 2.6403107911962407E-4 1.2081863837671176 >10k+ 1.3201553955981203E-4 1.7559121304682517 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35612 0.9177811200785109 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14036 0.36173132094299615 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11346 0.292405497821262 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9677 0.24939256146803745 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7941 0.20465292245713396 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6699 0.17264449408642998 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6516 0.16792827637963542 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5800 0.14947575245578354 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5335 0.13749192057786294 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5771681457893713E-5 2 0.0 7.731504437368114E-5 0.0 0.0 2.5771681457893713E-5 3 0.0 7.731504437368114E-5 0.0 0.0 2.5771681457893713E-5 4 0.0 1.0308672583157485E-4 0.0 0.0 2.5771681457893713E-5 5 0.0 1.0308672583157485E-4 0.0 0.0 2.5771681457893713E-5 6 0.0 1.0308672583157485E-4 0.0 0.0 2.5771681457893713E-5 7 0.0 1.0308672583157485E-4 0.0 0.0 2.5771681457893713E-5 8 0.0 1.0308672583157485E-4 0.0 0.0 2.5771681457893713E-5 9 0.0 1.0308672583157485E-4 0.0 2.5771681457893713E-5 2.5771681457893713E-5 10-11 0.0 1.0308672583157485E-4 0.0 3.865752218684057E-5 2.5771681457893713E-5 12-13 0.0 1.0308672583157485E-4 0.0 5.1543362915787426E-5 1.6751592947630912E-4 14-15 0.0 1.2885840728946857E-4 0.0 5.1543362915787426E-5 2.0617345166314968E-4 16-17 0.0 1.5463008874736227E-4 0.0 6.442920364473428E-5 2.8348849603683084E-4 18-19 0.0 1.5463008874736227E-4 0.0 9.020088510262799E-5 3.221460182236714E-4 20-21 0.0 1.5463008874736227E-4 0.0 1.8040177020525597E-4 3.6080354041051195E-4 22-23 0.0 1.5463008874736227E-4 0.0 2.4483097384999026E-4 3.6080354041051195E-4 24-25 0.0 1.8040177020525597E-4 0.0 5.798628328026086E-4 4.123469033262994E-4 26-27 0.0 1.8040177020525597E-4 0.0 0.001249926550707845 4.2523274405524623E-4 28-29 0.0 1.8040177020525597E-4 0.0 0.003105487615676192 4.381185847841931E-4 30-31 0.0 1.8040177020525597E-4 0.0 0.009045860191720692 4.381185847841931E-4 32-33 0.0 1.8040177020525597E-4 0.0 0.025926311546641073 4.381185847841931E-4 34-35 0.0 1.8040177020525597E-4 0.0 0.05149181955287163 4.638902662420868E-4 36-37 0.0 1.8040177020525597E-4 0.0 0.08861592669296753 4.896619476999805E-4 38-39 0.0 1.8040177020525597E-4 0.0 0.1421050515588259 4.896619476999805E-4 40-41 0.0 1.8040177020525597E-4 0.0 0.2055420454674313 4.896619476999805E-4 42-43 0.0 1.8040177020525597E-4 0.0 0.2722649287619181 5.025477884289274E-4 44-45 0.0 2.061734516631497E-4 0.0 0.35419310411656224 5.41205310615768E-4 46-47 0.0 2.061734516631497E-4 0.0 0.45463823259870295 5.798628328026086E-4 48-49 0.0 2.061734516631497E-4 0.0 0.5561528858613463 6.185203549894491E-4 50-51 0.0 2.061734516631497E-4 0.0 0.6680535267515209 6.442920364473427E-4 52-53 0.0 2.061734516631497E-4 0.0 0.7862295720766923 6.829495586341834E-4 54-55 0.0 2.061734516631497E-4 0.0 0.9253837661085895 6.958353993631302E-4 56-57 0.0 2.061734516631497E-4 0.0 1.0915209106269013 7.087212400920771E-4 58-59 0.0 2.061734516631497E-4 0.0 1.2713299321586258 7.216070808210239E-4 60-61 0.0 2.319451331210434E-4 0.0 1.461576484680797 7.216070808210239E-4 62-63 0.0 2.319451331210434E-4 0.0 1.6692962372314204 7.473787622789177E-4 64-65 0.0 2.4483097384999026E-4 0.0 1.8794900712020017 7.731504437368113E-4 66-67 1.2885840728946856E-5 2.5771681457893714E-4 0.0 2.135944073389502 7.860362844657582E-4 68-69 2.5771681457893713E-5 2.5771681457893714E-4 0.0 2.4267388411196453 7.989221251947051E-4 70-71 2.5771681457893713E-5 2.5771681457893714E-4 0.0 2.7323007823251624 7.989221251947051E-4 72-73 2.5771681457893713E-5 2.70602655307884E-4 0.0 3.032128524406298 9.277805324841735E-4 74-75 2.5771681457893713E-5 2.8348849603683084E-4 0.0 3.350254160322538 9.535522139420673E-4 76-77 3.865752218684057E-5 2.8348849603683084E-4 0.0 3.6941128201744844 9.535522139420673E-4 78-79 5.1543362915787426E-5 3.0926017749472454E-4 0.0 4.110235274834365 9.535522139420673E-4 80-81 5.1543362915787426E-5 3.0926017749472454E-4 0.0 4.535764393226378 9.79323895399961E-4 82-83 5.1543362915787426E-5 3.221460182236714E-4 0.0 4.9666282496801735 0.001056638939773642 84-85 5.1543362915787426E-5 3.3503185895261825E-4 0.0 5.413199945982556 0.001056638939773642 86-87 5.1543362915787426E-5 3.3503185895261825E-4 0.0 5.872696140536071 0.0011597256656052171 88 5.1543362915787426E-5 3.3503185895261825E-4 0.0 6.251024424337952 0.001262812391436792 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3380 0.0 35.337173 2 GTACCTG 4140 0.0 29.419594 1 ACCTGGG 4465 0.0 26.751932 3 GTACATG 12855 0.0 25.899921 1 TATCACG 370 0.0 25.418098 2 TACATGG 13265 0.0 24.92085 2 ACATGGG 13340 0.0 23.90104 3 CATGGGG 8910 0.0 22.375607 4 ATGGGGG 5835 0.0 20.14534 5 GAGTACT 12450 0.0 20.131329 12-13 AGAGTAC 18055 0.0 19.306082 10-11 TAACGCA 305 5.18412E-9 18.499844 4 AGTACTT 13205 0.0 17.575026 12-13 GTACTTT 14275 0.0 17.410652 14-15 TATAACG 310 1.254648E-7 16.685753 2 CCTGGGG 4215 0.0 16.621696 4 TGGGGGG 6170 0.0 16.447765 6 GAGTACA 10115 0.0 15.807032 1 GTGTAGC 1855 0.0 15.717558 1 ATAACGC 365 4.749927E-8 15.460776 3 >>END_MODULE