FastQCFastQC Report
Mon 6 Jun 2016
ERR523004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3880228
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT372080.9589127236853092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133770.34474778286224417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115900.2986937880969881No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT99180.25560353669938984No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC83050.21403381450780726No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC71500.18426752242394004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64320.16576345513717236No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA62200.1602998586680989No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG57690.14867683033058882No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC49530.12764713826094756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG37150.037.1958122
GTACCTG42200.033.0954171
ACCTGGG45100.029.384763
TATAACG3700.029.2167322
GTACATG130850.025.012631
TACATGG136550.023.5090222
ATAACGC4400.023.4973873
ACATGGG136000.022.7026233
CCTGGGG43450.021.7398134
TAACGCA4600.021.4541364
CATGGGG90850.021.0015184
GAGTACT133050.020.84860412-13
ATGGGGG58200.019.9445175
GAGTACG6150.019.8802621
AGAGTAC197500.018.59190210-11
GTACTTT149300.018.44941114-15
AGTACTT140200.017.77328712-13
TGGGGGG58900.017.2340766
TATAGCG2203.20076E-517.0890085
GTACACG5650.016.6458281
Produced by FastQC (version 0.11.5)