FastQCFastQC Report
Mon 6 Jun 2016
ERR523003_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523003_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7477715
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237430.3175167815301867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188950.2526841421477015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104950.14035036103943518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130200.046.4070931
GTATCAA222900.039.288611
TACCTGG73200.034.4198652
TCAACGC267500.032.2196164
CAACGCA271800.031.6921715
ATCAACG272650.031.5797333
TATCAAC280550.030.8795172
AACGCAG282850.030.6630636
GTACCTG91650.028.0856951
GTACATG255600.026.8979821
ACGCAGA322600.026.680947
ACCTGGG93650.026.5482793
TACATGG259150.025.8475342
CGCAGAG334450.025.734228
TATCACG7050.025.3366932
ACATGGG263850.024.599433
GCAGAGT367450.023.0387389
CATGGGG193100.022.5114924
GAGTACT216200.021.20946912-13
ATGGGGG118800.020.6487145
Produced by FastQC (version 0.11.5)