FastQCFastQC Report
Mon 6 Jun 2016
ERR523003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7477715
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216670.28975428991342944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183470.24535570023730513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100900.13493426802171518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129000.045.6241
GTATCAA221950.038.59941
TACCTGG73350.037.7039342
TCAACGC267750.031.4520554
ATCAACG272050.030.9391043
CAACGCA273700.030.7854865
TATCAAC276000.030.72532
GTACCTG93150.030.0510651
AACGCAG285050.029.8063776
ACCTGGG94050.028.6822993
ACGCAGA325350.026.1141877
CGCAGAG335000.025.3115868
GTACATG248350.025.1381031
TACATGG254800.024.0521832
ACATGGG257750.022.8279293
GCAGAGT372350.022.5070679
CATGGGG188100.021.3858014
TATAACG6650.021.2182252
ATGGGGG114950.020.6862645
CCTGGGG88450.020.4551854
Produced by FastQC (version 0.11.5)