FastQCFastQC Report
Mon 6 Jun 2016
ERR523002_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523002_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9584765
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1188081.2395504741117807No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349450.364589011832841No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT340730.3554912405259806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281390.29358048945383636No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC279320.2914208120908546No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC246520.25719983745037045No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA208880.21792918240561976No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG201660.21039639469512292No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA171400.17882545894448118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168930.1762484526224691No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG103500.10798386814908868No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT97930.10217256239459184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG406800.027.970941
TACATGG418150.026.9173852
ACATGGG422600.025.9455343
CATGGGG265550.024.2195994
ATGGGGG149800.022.0011165
GAGTACT291450.021.50425312-13
GTACTTT314500.019.7638114-15
AGAGTAC483350.019.60349710-11
GAGTACA311650.019.0248221
AGTACTT311400.018.74607512-13
GTATAGG30350.018.74561
AGTACAT303450.018.2979972
CATGGGA146800.017.2939764
CATGGGT68500.016.952494
ACTTTTT379650.016.65559416-17
CGTATAC6950.016.236413
TATAGGG17800.016.1120432
TACTTTT366250.015.81032314-15
TACCTGG57850.015.6040982
GTATTAC24550.015.5133711
Produced by FastQC (version 0.11.5)