##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523002_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9584765
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.212242449345396	34.0	31.0	34.0	30.0	34.0
2	32.514362323958906	34.0	31.0	34.0	30.0	35.0
3	32.39515616710477	34.0	31.0	34.0	30.0	35.0
4	34.98992734824485	37.0	35.0	37.0	33.0	37.0
5	35.007038878887485	37.0	35.0	37.0	33.0	37.0
6	35.18458386825342	37.0	35.0	37.0	33.0	37.0
7	35.0558182699315	37.0	35.0	37.0	33.0	37.0
8	35.20250825137601	37.0	35.0	37.0	33.0	37.0
9	36.49228854332892	39.0	35.0	39.0	33.0	39.0
10-11	36.59429594778797	39.0	35.5	39.0	33.0	39.0
12-13	36.66233188815792	39.0	37.0	39.0	33.0	39.0
14-15	37.7872854472697	40.0	37.5	41.0	33.5	41.0
16-17	37.69052183334698	40.0	36.5	41.0	33.5	41.0
18-19	37.73386812300562	40.0	36.5	41.0	34.0	41.0
20-21	37.76288698783955	40.0	36.0	41.0	34.0	41.0
22-23	37.671628829710485	40.0	36.5	41.0	33.5	41.0
24-25	37.67056177172836	40.0	37.0	41.0	33.0	41.0
26-27	37.49457394104081	39.0	36.5	41.0	33.5	41.0
28-29	37.33130687085182	39.0	37.0	41.0	32.5	41.0
30-31	37.25551122014989	39.0	36.5	41.0	33.0	41.0
32-33	37.08934840864643	39.0	36.0	41.0	32.0	41.0
34-35	36.985820935620225	38.5	35.5	41.0	32.0	41.0
36-37	36.86860434241215	38.0	36.0	41.0	31.5	41.0
38-39	36.63050309527672	38.0	35.5	41.0	31.5	41.0
40-41	36.61170571213796	38.0	35.5	41.0	31.0	41.0
42-43	36.563430350144216	38.0	35.5	40.5	31.0	41.0
44-45	36.45374784879964	38.0	35.5	40.0	31.0	41.0
46-47	36.161800106731874	38.0	35.0	40.0	30.0	41.0
48-49	36.25659804909145	38.0	36.0	40.0	30.0	41.0
50-51	35.22711501012283	37.5	34.0	39.0	28.5	40.0
52-53	35.37353664904669	37.5	35.0	39.5	28.5	40.0
54-55	35.95426950999842	38.0	35.0	40.0	30.0	41.0
56-57	36.083803202269436	38.0	35.0	41.0	29.5	41.0
58-59	36.08028251083881	38.0	35.0	41.0	30.0	41.0
60-61	35.79216798742588	37.5	35.0	40.0	29.0	41.0
62-63	35.60993128157028	37.5	35.0	40.0	29.0	41.0
64-65	35.37871799673753	37.0	35.0	40.0	29.0	41.0
66-67	35.122107219112834	37.0	35.0	39.0	28.0	41.0
68-69	34.77682066279142	37.0	34.5	39.0	28.0	41.0
70-71	34.36465009835922	36.0	34.0	38.5	27.0	41.0
72-73	34.10325198374713	36.0	34.0	38.0	26.5	40.0
74-75	33.851689425875335	36.0	34.0	37.0	26.0	39.0
76-77	33.5078490187292	35.5	34.0	37.0	26.0	39.0
78-79	33.20800562142108	35.0	33.5	37.0	25.5	39.0
80-81	32.96934937893626	35.0	33.0	36.0	25.0	38.5
82-83	32.72442370783217	35.0	33.0	36.0	25.0	38.0
84-85	32.432207675409884	35.0	33.0	36.0	24.5	37.0
86-87	32.37503647715933	35.0	33.0	36.0	24.5	37.0
88-89	32.06340979669298	35.0	32.0	36.0	22.0	37.0
90-91	31.96243282959989	35.0	32.5	35.5	23.0	37.0
92-93	31.732744673447915	35.0	32.0	35.0	21.0	36.0
94-95	31.636844617473667	35.0	32.0	35.0	20.0	36.0
96-97	31.37022279628139	35.0	31.0	35.0	19.5	36.0
98-99	31.34754284533841	35.0	32.0	35.0	19.0	36.5
100	30.120985543203197	34.0	29.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.06639084342427282
1101	2	0.05520737335945114
1101	3	0.03339341803600604
1101	4	0.05887368483456612
1101	5	0.06371762874151443
1101	6	0.010766924539865386
1101	7	0.11888127208606392
1101	8	0.01863151514110939
1101	9	-0.027348951763542573
1101	10-11	-0.024139396921611933
1101	12-13	-0.03339305364335132
1101	14-15	0.03492153212098259
1101	16-17	-0.04980067068137828
1101	18-19	-0.04622306371046392
1101	20-21	-0.012424856784093663
1101	22-23	-0.0570375025227321
1101	24-25	-0.12746774897892266
1101	26-27	-0.1262243583302336
1101	28-29	-0.11148989253427999
1101	30-31	-0.14481231224417712
1101	32-33	-0.11070216036257108
1101	34-35	-0.24198636250724093
1101	36-37	-0.10294005157702202
1101	38-39	-0.16540059934727935
1101	40-41	-0.1317393431787437
1101	42-43	-0.13103579472410587
1101	44-45	-0.1906091946224464
1101	46-47	-0.2197876289530356
1101	48-49	-0.2470878509359622
1101	50-51	-0.35330123775504063
1101	52-53	-0.30361459604373664
1101	54-55	-0.19922733510745871
1101	56-57	-0.14635490199501078
1101	58-59	-0.10329338467035143
1101	60-61	-0.13527340020490897
1101	62-63	-0.05314387318353653
1101	64-65	-0.04473574128513036
1101	66-67	-0.37911182721502
1101	68-69	-0.21723053035641726
1101	70-71	-0.24957065563497594
1101	72-73	-0.15240825674374747
1101	74-75	-0.26978001826371667
1101	76-77	-0.21259868145903482
1101	78-79	-0.4634113723961022
1101	80-81	-0.20995007373621632
1101	82-83	-0.2548395065228348
1101	84-85	-0.562326963337263
1101	86-87	-0.7731060429255479
1101	88-89	-0.325818675678093
1101	90-91	-0.4236923572026683
1101	92-93	-0.21803493102501648
1101	94-95	-0.45325423550776733
1101	96-97	-0.4393001589507328
1101	98-99	-0.42962534557047505
1101	100	-0.5191766896689707
1102	1	-0.07674577259835047
1102	2	-0.13304498571543633
1102	3	0.0012474707310232702
1102	4	-0.055732831436621666
1102	5	-0.003296240521933669
1102	6	0.0071851273218825895
1102	7	0.12303989574627394
1102	8	-0.02267477496476289
1102	9	-0.0866176193441035
1102	10-11	-0.01226962191407921
1102	12-13	-0.06563839468039134
1102	14-15	-0.03406050386207937
1102	16-17	-0.06776055457088148
1102	18-19	-0.10132437721648557
1102	20-21	-0.11073540409909555
1102	22-23	-0.15868353816368597
1102	24-25	-0.05639905225493891
1102	26-27	-0.11232953993145145
1102	28-29	-0.4124448152784197
1102	30-31	-0.2204460870127818
1102	32-33	-0.22820457168810293
1102	34-35	-0.232490681224796
1102	36-37	-0.29436340834164554
1102	38-39	-0.3254854431531271
1102	40-41	-0.7714275945862425
1102	42-43	-0.2562070638993461
1102	44-45	-0.36837120134973844
1102	46-47	-0.335392599660004
1102	48-49	-0.6325770252795309
1102	50-51	-0.4319553862037324
1102	52-53	-0.4847739836008671
1102	54-55	-0.24960945553993952
1102	56-57	-0.16425209657983686
1102	58-59	-0.1530551584337303
1102	60-61	-0.567377071209421
1102	62-63	-0.1479666457728186
1102	64-65	-0.1666306802931885
1102	66-67	-0.20428795171588376
1102	68-69	-0.4259916645008701
1102	70-71	-0.0923561748641788
1102	72-73	-0.38344212811441736
1102	74-75	-0.18034857173552865
1102	76-77	-0.37681604366782295
1102	78-79	-0.248501567378419
1102	80-81	-0.2164036613517979
1102	82-83	-0.25597980296253553
1102	84-85	-0.2997543641811333
1102	86-87	-0.24136350037896648
1102	88-89	-0.2312429973168726
1102	90-91	-0.28339532133597345
1102	92-93	-0.2810175271762283
1102	94-95	-0.4311248593660899
1102	96-97	-0.3433977556963903
1102	98-99	-0.44501120752720524
1102	100	-0.728453411243045
1103	1	-0.16262981300863544
1103	2	-0.11207989814390373
1103	3	-0.15351371669451908
1103	4	-0.06498571601029823
1103	5	-0.11195916695434249
1103	6	-0.17588660659631472
1103	7	0.011288271187105181
1103	8	-0.12621004747120423
1103	9	-0.15007907504229934
1103	10-11	-0.137789772247352
1103	12-13	-0.11109863791597263
1103	14-15	-0.18303438755192758
1103	16-17	-0.22911315721457015
1103	18-19	-0.27220811657267063
1103	20-21	-0.3214259074964332
1103	22-23	-0.26552404512812444
1103	24-25	-0.3721326259257367
1103	26-27	-0.3347251870877699
1103	28-29	-0.44357680835894087
1103	30-31	-0.3444259375484222
1103	32-33	-0.5034189433943155
1103	34-35	-0.49509440702860985
1103	36-37	-0.5598654202360862
1103	38-39	-0.41483606961882913
1103	40-41	-0.3805750074297123
1103	42-43	-0.3739763176099018
1103	44-45	-0.35359896312019856
1103	46-47	-0.3697452526327183
1103	48-49	-0.41150652163390333
1103	50-51	-0.49001883559682113
1103	52-53	-0.3944291914058411
1103	54-55	-0.30320545737787086
1103	56-57	-0.3121670149301181
1103	58-59	-0.2065834642721711
1103	60-61	-0.23814638615304773
1103	62-63	-0.25716964657291186
1103	64-65	-0.22565448149102707
1103	66-67	-0.23324001434995267
1103	68-69	-0.24749862788061705
1103	70-71	-0.24968047935338689
1103	72-73	-0.1813964436054718
1103	74-75	-0.2044946045915097
1103	76-77	-0.3369819002295671
1103	78-79	-0.3093404618832025
1103	80-81	-0.3327963975574235
1103	82-83	-0.26365534235227983
1103	84-85	-0.30406923449451284
1103	86-87	-0.42462154595479973
1103	88-89	-0.28558593250614095
1103	90-91	-0.3091350838522722
1103	92-93	-0.29365827848914705
1103	94-95	-0.5043983674578563
1103	96-97	-0.3938136130163059
1103	98-99	-0.4221126949502967
1103	100	-0.4639095305882748
1104	1	-0.005775495906370054
1104	2	-0.035679970613742285
1104	3	-0.008689472080860128
1104	4	-0.03827583864473638
1104	5	-0.08381271479600372
1104	6	-0.0397350802437515
1104	7	0.05681411480558296
1104	8	-0.05666810820969914
1104	9	-0.08536736266470513
1104	10-11	-0.1728389730456783
1104	12-13	-0.055868366961746574
1104	14-15	-0.052102054544512555
1104	16-17	-0.08159453640470105
1104	18-19	-0.17335809443785166
1104	20-21	-0.15963659626611104
1104	22-23	-0.17681324371733353
1104	24-25	-0.1547348710569949
1104	26-27	-0.12206608684327591
1104	28-29	-0.16607843450672277
1104	30-31	-0.15144195207493993
1104	32-33	-0.17466902351713287
1104	34-35	-0.20622581481816127
1104	36-37	-0.24657100596791537
1104	38-39	-0.3249735279032393
1104	40-41	-0.12234089709635754
1104	42-43	-0.14695423199177782
1104	44-45	-0.09753443694189201
1104	46-47	-0.21898605670476456
1104	48-49	-0.17831292497336193
1104	50-51	-0.22884240108840714
1104	52-53	-0.19460431169613202
1104	54-55	-0.19500175903586126
1104	56-57	-0.7069303988435607
1104	58-59	-0.0765657581649748
1104	60-61	-0.0820865831264257
1104	62-63	-0.12292902090124613
1104	64-65	-0.11217922949450809
1104	66-67	-0.11008856668938449
1104	68-69	-0.12130436668009281
1104	70-71	-0.13800051456301077
1104	72-73	-0.16001359244837943
1104	74-75	-0.15754247290621493
1104	76-77	-0.25254068456725065
1104	78-79	-0.18578720246735259
1104	80-81	-0.14254791187131133
1104	82-83	-0.03868508847482843
1104	84-85	-0.15435167302042174
1104	86-87	-0.14443639001625286
1104	88-89	-0.18795674367119375
1104	90-91	-0.3987888137730664
1104	92-93	-0.4988169042911963
1104	94-95	-0.2328751826287494
1104	96-97	-0.2937190893696382
1104	98-99	-0.2983408557211078
1104	100	-0.2675768806937455
1105	1	-0.024789209019843383
1105	2	-0.06429432004284052
1105	3	-0.013684616650067483
1105	4	0.002930909073455723
1105	5	-0.0012389618475623365
1105	6	-0.012821020222929747
1105	7	0.08155424531174305
1105	8	0.028389086627655047
1105	9	-0.002020208787143929
1105	10-11	-0.05351819947684788
1105	12-13	0.002254837975215196
1105	14-15	0.010654476153725057
1105	16-17	-0.025279431644278816
1105	18-19	-0.04783209528149257
1105	20-21	-0.06849766041621308
1105	22-23	-0.09861347670692311
1105	24-25	-0.09970584276941707
1105	26-27	-0.15236986334089409
1105	28-29	-0.13229498522346717
1105	30-31	-0.11084975087474902
1105	32-33	-0.09210675845761074
1105	34-35	-0.17643894655208925
1105	36-37	-0.15315322957702193
1105	38-39	-0.054197233546133816
1105	40-41	-0.1109920905529691
1105	42-43	-0.16019021514203047
1105	44-45	-0.2579216364426884
1105	46-47	-0.1776861196993238
1105	48-49	-0.17525458001416183
1105	50-51	-0.23778639785791
1105	52-53	-0.18439611513590393
1105	54-55	-0.23278195303140592
1105	56-57	-0.12268282832452115
1105	58-59	-0.06396597605719734
1105	60-61	-0.04708269910918261
1105	62-63	-0.04614770124459966
1105	64-65	-0.12007984532953486
1105	66-67	-0.17127129168258648
1105	68-69	-0.07870349787064157
1105	70-71	-0.15743186067045656
1105	72-73	-0.1278983600244743
1105	74-75	-0.11460020625370504
1105	76-77	-0.11465198643772823
1105	78-79	-0.11314230037503847
1105	80-81	-0.06182070406509155
1105	82-83	-0.10692309606588424
1105	84-85	-0.11947410901131406
1105	86-87	-0.08615060660442708
1105	88-89	-0.07651312492047069
1105	90-91	-0.11717734135566715
1105	92-93	-0.2028530831553219
1105	94-95	-0.1631799611742899
1105	96-97	-0.1258800090985588
1105	98-99	-0.14760807807187248
1105	100	-0.0804204387276144
1106	1	0.017344351696671367
1106	2	0.021571636953375162
1106	3	0.015363846774370415
1106	4	0.05995160879466255
1106	5	0.07454039159295434
1106	6	0.059690796688038006
1106	7	0.15789489446933658
1106	8	0.04653175422057387
1106	9	0.05714978932784476
1106	10-11	0.03228190408912468
1106	12-13	0.10375449107665702
1106	14-15	0.0826338701235585
1106	16-17	0.0017339039523278643
1106	18-19	0.0024526921273633207
1106	20-21	0.003368447076546488
1106	22-23	-0.021191961752819566
1106	24-25	0.024534557021397063
1106	26-27	-0.00443229496669062
1106	28-29	-0.015217081818526879
1106	30-31	-0.03457378568806746
1106	32-33	-0.05351337496980335
1106	34-35	-0.051239388668143704
1106	36-37	-0.027450009617901117
1106	38-39	-0.09521431870913943
1106	40-41	-0.01748018603886692
1106	42-43	0.06217342959247674
1106	44-45	-0.0347002912067893
1106	46-47	0.017374605567802348
1106	48-49	-0.019849745198449398
1106	50-51	-0.0787946298220632
1106	52-53	-0.04681612355781084
1106	54-55	-0.021858552640267703
1106	56-57	-0.006009496299192563
1106	58-59	0.05776655455925095
1106	60-61	0.0065642167434134535
1106	62-63	0.02187688340932681
1106	64-65	0.09512308798334601
1106	66-67	0.026011535018582777
1106	68-69	0.10649983787054396
1106	70-71	0.06955145619195946
1106	72-73	0.12967809698869104
1106	74-75	0.0802431002959807
1106	76-77	0.09236474831273256
1106	78-79	0.10141886834659175
1106	80-81	0.05787099131432427
1106	82-83	0.022732236178519827
1106	84-85	0.10119667339122174
1106	86-87	-0.006141738409631614
1106	88-89	0.03958841423126103
1106	90-91	0.01943009650418759
1106	92-93	-0.03480544728283874
1106	94-95	0.019685602973115834
1106	96-97	-0.035996231869798834
1106	98-99	-0.05886804326428674
1106	100	0.06529458616810402
1107	1	-0.09822795345277768
1107	2	-0.10425575779932927
1107	3	-0.01287452391930799
1107	4	0.007958722509485483
1107	5	-0.030985643261018936
1107	6	0.022783474993772757
1107	7	0.12326011850542784
1107	8	0.01606109354248275
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2316	72-73	0.3746620574561348
2316	74-75	0.4236048969071575
2316	76-77	-1.0741027408422994
2316	78-79	0.34742122402450804
2316	80-81	-0.714979482506422
2316	82-83	0.3470300862004905
2316	84-85	-1.2387311926004543
2316	86-87	0.4499452334729668
2316	88-89	0.5742410470949366
2316	90-91	-0.3655680689700347
2316	92-93	0.48693175805693656
2316	94-95	0.5122284033212559
2316	96-97	0.6921245396540172
2316	98-99	0.6042628173533338
2316	100	0.5618931926466857
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	22009.0
1	1763.0
2	1222.0
3	957.0
4	813.0
5	921.0
6	1072.0
7	1438.0
8	2310.0
9	3105.0
10	4317.0
11	6414.0
12	9410.0
13	12519.0
14	14821.0
15	17288.0
16	20251.0
17	24657.0
18	31000.0
19	39100.0
20	48506.0
21	61221.0
22	73968.0
23	81739.0
24	84092.0
25	84128.0
26	86941.0
27	94906.0
28	108549.0
29	128937.0
30	158200.0
31	200656.0
32	265665.0
33	372251.0
34	595740.0
35	1135480.0
36	1606587.0
37	1619963.0
38	1988770.0
39	572192.0
40	887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.215019157152206	18.6874781568529	13.38411101645239	22.713391669542496
2	15.24957405015615	21.611316965529237	39.242280145756844	23.89682883855777
3	20.595308006329933	25.73743835307738	29.8809451006247	23.786308539967983
4	12.491731617384122	16.326910356334437	39.290757327826284	31.890600698455163
5	12.72934759000324	38.86856899419349	34.6259907126806	13.776092703122671
6	29.95171382385262	37.30487768761321	17.74621884214687	14.997189646387296
7	26.625892614909517	33.13973107673139	22.551201848198527	17.68317446016057
8	25.36079545959996	33.21644776219687	20.603669582474136	20.81908719572903
9	25.96134219982687	16.931168139240096	21.40323460235284	35.704255058580195
10-11	25.05130773506749	25.490411114869048	28.40332588814809	21.054955261915367
12-13	26.793884849340156	23.696614340299455	27.80634215517613	21.70315865518426
14-15	23.18132349676566	25.301008398867985	27.305228420757604	24.212439683608753
16-17	21.82106537007091	28.23027643587679	27.456379868780317	22.492278325271986
18-19	21.565987106300053	28.56503100766391	28.53968295997379	21.329298926062247
20-21	23.11532772795436	26.235699082916014	28.145830210938527	22.5031429781911
22-23	22.428671147907096	26.20118592752363	28.51863712522461	22.85150579934466
24-25	23.310978475076322	26.323039214147194	28.190878673611742	22.175103637164742
26-27	22.549528631992853	25.68360330003388	29.790002838541906	21.976865229431365
28-29	21.682143510611706	27.909557339304534	28.58253679418411	21.82576235589965
30-31	22.617302798955556	26.146414079062154	28.331109489763822	22.905173632218467
32-33	22.640026779288707	26.52738255006164	28.59872201841406	22.233868652235593
34-35	23.492847581210697	26.27883274099418	28.447978262913814	21.780341414881317
36-37	22.893228548897163	25.87268859516019	29.383936405893003	21.850146450049646
38-39	22.34950282358022	27.060105084433168	28.88295793932602	21.70743415266059
40-41	22.44747642418012	27.14593055983146	28.326565551544856	22.08002746444356
42-43	21.90237336793046	27.235654437847028	27.460043475277878	23.40192871894463
44-45	22.54164644930868	26.211883772181473	27.915704069262954	23.330765709246894
46-47	23.36578583546398	26.94743793557084	27.858825597933645	21.827950631031534
48-49	22.83786205562427	26.877670902146267	27.946858730160056	22.337608312069403
50-51	21.504508657802603	27.191889717457162	28.998084708160697	22.305516916579535
52-53	22.55400916311638	27.887517901496732	27.134007344501775	22.424465590885106
54-55	21.804623730784733	26.679050067548914	27.966177956307497	23.550148245358855
56-57	22.843225565766897	26.40829597048764	27.61577177149885	23.132706692246618
58-59	23.930067673743086	26.440645033095667	27.30537438942145	22.3239129037398
60-61	22.82164946959058	26.92736426715324	28.74415907996249	21.50682718329369
62-63	22.02804511352584	27.099859200177228	28.450473791900933	22.421621894395997
64-65	21.771142999402425	28.051673395480464	27.659800777976784	22.517382827140324
66-67	22.510046653570157	26.819516975549817	27.015064406295664	23.65537196458436
68-69	21.892901620232724	27.031901943508768	27.643687197766216	23.431509238492293
70-71	23.114621958974364	26.741020911129976	27.351453722841224	22.79290340705444
72-73	22.63310459543025	26.28897207212561	28.433944240587632	22.643979091856508
74-75	21.718319152536125	28.414447828403137	27.357944931935524	22.50928808712521
76-77	22.157095124029077	26.74272375095237	27.24950636052191	23.85067476449665
78-79	22.377356467785866	26.91056268782225	27.641959700087444	23.07012114430444
80-81	23.133779466099252	26.82640301962877	27.171167041369955	22.868650472902026
82-83	22.694195604542085	26.406088046855647	28.23143193824581	22.668284410356456
84-85	22.17103347138765	27.357700493350784	27.747158470268623	22.724107564992945
86-87	22.096103465998063	27.73739223840628	27.29827050072851	22.868233794867148
88-89	21.82052237846933	27.482270329583237	26.73330531393081	23.963901978016615
90-91	22.579657207595496	26.414286323825397	27.07999266009513	23.926063808483978
92-93	23.334899608103292	27.104809744200654	27.135215748897174	22.425074898798883
94-95	22.811284920102786	26.7484703431831	27.340716144980544	23.099528591733574
96-97	21.498661661462396	26.89673006841072	28.481269281714162	23.123338988412726
98-99	22.323150582928122	27.449140215771017	26.839362570249975	23.388346631050887
100	22.307778426745397	25.68014093105105	26.948874372820274	25.063206269383283
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14471.0
1	11118.5
2	5867.0
3	3300.5
4	2311.0
5	2250.5
6	3122.0
7	5436.0
8	8964.0
9	10997.5
10	10205.5
11	9214.0
12	9525.0
13	10239.0
14	11110.0
15	11778.0
16	12272.0
17	12934.0
18	13720.5
19	14529.5
20	15637.0
21	17375.0
22	19698.0
23	23171.5
24	27851.0
25	34096.5
26	42933.0
27	53193.0
28	63770.0
29	77364.0
30	94763.0
31	111516.0
32	131766.5
33	153896.0
34	173949.0
35	193569.0
36	219779.0
37	245331.5
38	259798.0
39	271866.5
40	277993.0
41	281596.5
42	288127.0
43	297097.0
44	307529.0
45	321142.0
46	339919.5
47	422590.0
48	589937.5
49	575075.0
50	408840.5
51	336998.5
52	316283.0
53	305230.5
54	292876.0
55	274763.0
56	251464.0
57	226087.5
58	200757.0
59	173037.0
60	145492.0
61	122315.0
62	101270.0
63	79630.5
64	59454.5
65	44882.0
66	33446.5
67	25134.0
68	19307.0
69	14534.5
70	11211.0
71	8488.5
72	6354.0
73	4933.0
74	3756.0
75	2866.0
76	2083.5
77	1346.5
78	939.5
79	672.5
80	445.5
81	295.0
82	220.5
83	170.5
84	139.0
85	107.5
86	87.5
87	68.5
88	55.0
89	53.5
90	44.5
91	38.0
92	31.0
93	23.0
94	19.5
95	17.5
96	13.5
97	9.5
98	7.5
99	2.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20248801092149887
2	0.1807451721560205
3	0.36746858165015
4	0.36341005752358035
5	0.373832848275362
6	0.30285562556828466
7	0.3214789303650116
8	0.3112856705406966
9	0.3254644219237509
10-11	0.299443961328212
12-13	0.3072427962500906
14-15	0.2940030350248546
16-17	0.2894019832515456
18-19	0.31079009240184813
20-21	0.31438433806149657
22-23	0.3064759542878725
24-25	0.31085790835769056
26-27	0.3005029335617514
28-29	0.32711287131192057
30-31	0.3089434117581391
32-33	0.3090268775499451
34-35	0.3114369522883451
36-37	0.3180411830649995
38-39	0.3246193307817145
40-41	0.349580819143714
42-43	0.31925143704618736
44-45	0.3050518192151816
46-47	0.31064924387817544
48-49	0.33191215434076893
50-51	0.32205797429566607
52-53	0.34991468231093825
54-55	0.34991468231093825
56-57	0.3553138757183927
58-59	0.3417819842218354
60-61	0.3250418763527327
62-63	0.34702467926965347
64-65	0.34230364542062325
66-67	0.32952294605032045
68-69	0.3396744729787324
70-71	0.35986276137182294
72-73	0.3640934336939925
74-75	0.35286206808408965
76-77	0.3364401735462476
78-79	0.346184804739605
80-81	0.3580630302360048
82-83	0.3634778734794228
84-85	0.3553086591064048
86-87	0.3558824864250715
88-89	0.34482326901076865
90-91	0.3571553397501139
92-93	0.3377808428271324
94-95	0.35826126149154414
96-97	0.36764594645773785
98-99	0.3580317305640775
100	0.37837130070481645
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9584765.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.82204687679325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.97108700640823	31.603963819510234
2	16.13672818226148	14.788358304113123
3	6.065578029177419	8.338116023634445
4	2.979177111732266	5.460479730722616
5	1.642024393343414	3.7620459362309964
6	1.0214132936539138	2.808194869343365
7	0.642567089873269	2.061061751956029
8	0.47315085034209226	1.734459235533595
9	0.33592005756067383	1.3853290161960254
>10	1.5899928118554545	12.68601099176792
>50	0.08487441775232872	2.648477817394906
>100	0.051706283705138315	4.5013629146340985
>500	0.0036043206620076504	1.1137328258732224
>1k	0.0017001184942232067	1.273307472167857
>5k	1.0200710956729404E-4	0.31979144256420994
>10k+	3.7402606841341145E-4	5.515307848357341
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	118808	1.2395504741117807	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34945	0.364589011832841	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	34073	0.3554912405259806	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28139	0.29358048945383636	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	27932	0.2914208120908546	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	24652	0.25719983745037045	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	20888	0.21792918240561976	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	20166	0.21039639469512292	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	17140	0.17882545894448118	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16893	0.1762484526224691	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	10350	0.10798386814908868	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	9793	0.10217256239459184	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.086644795151472E-5
2	0.0	0.0	0.0	0.0	2.086644795151472E-5
3	0.0	0.0	0.0	0.0	2.086644795151472E-5
4	0.0	0.0	0.0	0.0	2.086644795151472E-5
5	0.0	0.0	0.0	0.0	2.086644795151472E-5
6	0.0	0.0	0.0	0.0	6.259934385454416E-5
7	0.0	0.0	0.0	0.0	6.259934385454416E-5
8	0.0	0.0	0.0	0.0	8.346579180605889E-5
9	0.0	0.0	0.0	7.303256783030152E-5	1.1476546373333097E-4
10-11	0.0	0.0	0.0	8.868240379393757E-5	1.1476546373333097E-4
12-13	0.0	0.0	0.0	1.1998207572120965E-4	1.1476546373333097E-4
14-15	0.0	0.0	0.0	1.4084852367272438E-4	2.190977034909046E-4
16-17	0.0	0.0	0.0	2.1909770349090457E-4	2.8169704734544877E-4
18-19	0.0	0.0	0.0	2.973468833090848E-4	2.8169704734544877E-4
20-21	0.0	0.0	0.0	4.329787949939305E-4	3.129967192727209E-4
22-23	0.0	0.0	0.0	6.312100505333204E-4	3.9646251107877976E-4
24-25	0.0	0.0	0.0	9.076904858908904E-4	4.4862863095756654E-4
26-27	0.0	0.0	0.0	0.001403268624739365	4.799283028848386E-4
28-29	0.0	0.0	0.0	0.0031247505807393294	4.799283028848386E-4
30-31	0.0	0.0	0.0	0.009802013925224042	5.112279748121106E-4
32-33	0.0	0.0	0.0	0.02710029927702974	5.425276467393828E-4
34-35	0.0	0.0	0.0	0.05322509211232618	5.946937666181695E-4
36-37	0.0	1.043322397575736E-5	0.0	0.09171847197088295	6.155602145696843E-4
38-39	0.0	1.043322397575736E-5	0.0	0.1472284401339	6.36426662521199E-4
40-41	0.0	1.043322397575736E-5	0.0	0.2061448559250018	6.572931104727138E-4
42-43	0.0	2.086644795151472E-5	0.0	0.2682851379246126	6.729429464363498E-4
44-45	0.0	2.086644795151472E-5	0.0	0.34859487947800494	6.990260063757432E-4
46-47	0.0	2.086644795151472E-5	0.0	0.44640635425073016	7.5119212625453E-4
48-49	0.0	2.086644795151472E-5	0.0	0.5452611514210312	8.242246940848315E-4
50-51	0.0	2.086644795151472E-5	0.0	0.651001876415332	8.294413060727101E-4
52-53	0.0	2.086644795151472E-5	0.0	0.7600655832459116	9.076904858908904E-4
54-55	0.0	2.086644795151472E-5	0.0	0.887110951598709	9.859396657090707E-4
56-57	0.0	2.086644795151472E-5	0.0	1.0461706677211178	0.0010015895016727066
58-59	0.0	2.086644795151472E-5	0.0	1.2128101210619144	0.0010172393376363426
60-61	0.0	2.086644795151472E-5	0.0	1.3923554724607228	0.0010328891735999787
62-63	0.0	2.086644795151472E-5	0.0	1.5769348544278343	0.0010537556215514934
64-65	0.0	2.086644795151472E-5	0.0	1.7693391543767636	0.0010537556215514934
66-67	0.0	2.086644795151472E-5	0.0	1.998635334303971	0.0010798386814908868
68-69	0.0	2.086644795151472E-5	0.0	2.2627158829663534	0.0011007051294424017
70-71	0.0	2.086644795151472E-5	0.0	2.5480176091954263	0.0011163549654060376
72-73	0.0	2.086644795151472E-5	0.0	2.8308205782823053	0.0012832865490181554
74-75	0.0	2.086644795151472E-5	0.0	3.1343491467970264	0.0012937197729939128
76-77	0.0	2.086644795151472E-5	0.0	3.4498550564359167	0.0013302360569090634
78-79	0.0	2.086644795151472E-5	0.0	3.8425250905995085	0.0013563191168484568
80-81	0.0	2.086644795151472E-5	0.0	4.2584612142290394	0.0013928354007636077
82-83	0.0	2.086644795151472E-5	0.0	4.662435646570364	0.0014606513566060306
84-85	0.0	2.086644795151472E-5	0.0	5.081887766679726	0.0014606513566060306
86-87	0.0	2.086644795151472E-5	0.0	5.512832082998384	0.0014815178045575453
88	0.0	2.086644795151472E-5	0.0	5.862668516129504	0.00150238425250906
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	40680	0.0	27.97094	1
TACATGG	41815	0.0	26.917385	2
ACATGGG	42260	0.0	25.945534	3
CATGGGG	26555	0.0	24.219599	4
ATGGGGG	14980	0.0	22.001116	5
GAGTACT	29145	0.0	21.504253	12-13
GTACTTT	31450	0.0	19.76381	14-15
AGAGTAC	48335	0.0	19.603497	10-11
GAGTACA	31165	0.0	19.024822	1
AGTACTT	31140	0.0	18.746075	12-13
GTATAGG	3035	0.0	18.7456	1
AGTACAT	30345	0.0	18.297997	2
CATGGGA	14680	0.0	17.293976	4
CATGGGT	6850	0.0	16.95249	4
ACTTTTT	37965	0.0	16.655594	16-17
CGTATAC	695	0.0	16.23641	3
TATAGGG	1780	0.0	16.112043	2
TACTTTT	36625	0.0	15.810323	14-15
TACCTGG	5785	0.0	15.604098	2
GTATTAC	2455	0.0	15.513371	1
>>END_MODULE