FastQCFastQC Report
Mon 6 Jun 2016
ERR523002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9584765
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1213531.266103029130083No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT338690.3533628628349261No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323330.33733743080816275No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC292800.30548479801017553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274020.2858912033837032No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC253350.2643257294258127No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA216610.2259940645388802No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG209320.21838824426055306No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA181100.1889456862009658No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC163510.17059364522760861No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157960.16480320592106326No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG401050.027.440951
TACATGG411500.026.4201952
ACATGGG414600.025.4697513
CATGGGG260250.023.1137354
GAGTACT295450.021.25891312-13
ATGGGGG151050.021.000165
GTACTTT311950.020.00307514-15
AGAGTAC485600.019.25628310-11
CATGGGA147400.019.065784
AGTACTT313550.018.32242812-13
GAGTACA311600.017.9761281
AGTACAT301200.017.0411112
ACTTTTT374900.016.74135816-17
GTATAGG28650.015.9231471
TACTTTT360600.015.831387514-15
GTATATA49550.014.5221051
ATGGGGA97500.014.4595985
CATGGGT67950.014.3854944
TACCTGG58750.014.0010432
TGGGGGG138450.013.9504586
Produced by FastQC (version 0.11.5)