FastQCFastQC Report
Mon 6 Jun 2016
ERR523001_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523001_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6472955
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT666561.0297615231374233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219530.339149584695089No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT184530.285078453349359No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170190.26292473839227987No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC138920.21461604475853766No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC129210.1996151680337651No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG102350.15811943694958486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101850.1573469922160744No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA100150.15472068012213896No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA92960.143612924854259No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG223550.028.2247071
TACATGG227250.026.9756162
ACATGGG228300.026.2352263
CATGGGG152400.024.0296154
ATGGGGG91000.022.3184285
TATAACG4700.020.0045972
GAGTACT189350.019.88648812-13
AGAGTAC286400.018.29719210-11
CATGGGA86500.018.1520084
TACCTGG43800.018.1388722
GTACTTT210800.017.43921314-15
GAGTACA171050.017.4268051
AGTACTT202200.017.08918612-13
GTATAGA20500.016.0544621
AGTACAT167500.015.969642
GTATAGC19450.015.71250251
GTAATAC12600.015.2979983
GTATATG22200.015.248641
GTACCTG56300.015.1154741
ATAACGC7200.015.0181563
Produced by FastQC (version 0.11.5)