FastQCFastQC Report
Mon 6 Jun 2016
ERR523001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6472955
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT681361.0526258872493321No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203070.31372070406792574No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT184240.28463043540392297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166860.2577802564671004No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC143860.2222477987256207No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC136370.21067657661763445No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG107850.16661632901819956No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA103660.16014324215138218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99150.1531757906551181No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA96080.14843297999136407No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC87030.1344517303148253No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG217300.026.966541
TACATGG223400.025.8365042
ACATGGG226050.024.4488033
CATGGGG148800.023.150264
ATGGGGG88650.019.8791265
GAGTACT196750.019.48671212-13
TACCTGG44400.019.2666842
AGAGTAC295100.018.3531610-11
GAGTACA174100.017.9094261
GTACTTT216500.017.53180714-15
AGTACTT207100.017.50264212-13
AGTACAT166950.017.1454722
GAGTACG8450.016.6967321
GTATAGG17450.015.9009661
GTACCTG55750.015.6904351
TATACCG5450.015.5210365
GGGTTAG19400.015.0299281
CATGGGT39700.014.7970214
ACTTTTT262850.014.65196316-17
CATGGGA86150.014.6195614
Produced by FastQC (version 0.11.5)