FastQCFastQC Report
Mon 6 Jun 2016
ERR523000_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523000_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7841047
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT897831.145038411324406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286640.36556342539459336No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT257640.32857856865288526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217160.27695281000101135No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC188640.24058011640537288No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC176860.22555661252891354No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG146110.18633991098382652No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA140830.17960611637706037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132670.16919934289387628No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA129600.1652840494388058No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC115880.14778638618031495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG308250.028.840731
TACATGG315700.027.651342
ACATGGG320250.026.3208923
CATGGGG214400.024.8640984
ATGGGGG132900.023.8416355
GAGTACT236550.020.74589712-13
TACCTGG55950.020.5006982
AGAGTAC375800.018.8275610-11
GTACTTT262650.018.56687714-15
TGGGGGG131100.018.0270446
AGTACTT248600.018.0015812-13
CATGGGA110100.017.804644
GAGTACA233650.017.4651261
GTACCTG71200.017.3657511
TATCACG5600.016.7888452
AGTACAT227250.016.5694272
GTATAGG20850.016.23471
ACTTTTT315900.015.53255816-17
TATACCG4301.3951649E-915.3059645
GTATAGC26650.014.99476051
Produced by FastQC (version 0.11.5)