FastQCFastQC Report
Mon 6 Jun 2016
ERR523000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7841047
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT904081.153009285622188No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259970.3315501105910984No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT256920.32766032393378075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212550.27107349311896745No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC196280.25032371314698154No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC181310.23123187502893428No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG151930.19376238912992105No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA143680.1832408350568489No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA136510.1740966480624335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126930.16187889193879337No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC116090.1480542075567204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG312400.027.1006581
TACATGG317150.026.349832
ACATGGG327050.024.945113
CATGGGG213700.023.4204354
TACCTGG53600.022.3607392
ATGGGGG130850.022.0154885
GAGTACT242300.020.51641712-13
AGAGTAC392750.019.01259410-11
GTACTTT264750.018.64113814-15
GTACCTG66400.018.6297071
AGTACTT255200.018.622812-13
TATCACG7750.016.9811522
TGGGGGG127250.016.9141146
GAGTACA246100.016.8950311
AGTACAT236700.016.3819832
CATGGGA117600.016.2243984
ACTTTTT319050.015.56954116-17
TACTTTT302750.015.53288214-15
GGGTTAG23400.015.0752231
ACCTGGG77400.015.0577673
Produced by FastQC (version 0.11.5)