FastQCFastQC Report
Mon 6 Jun 2016
ERR522999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17254375
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT526210.3049719274097149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435700.25251566631651395No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT266220.15429130293041618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265260.15373492230231464No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG245000.047.175982
GTACCTG289950.040.488241
ACCTGGG295000.038.670093
CCTGGGG278250.028.1346324
CATGGGG460200.024.7553424
GTACATG784100.024.4315151
TACATGG812550.023.3679872
ATGGGGG273050.022.9522295
ACATGGG819050.022.4534873
TGGGGGG268450.021.9511686
GTATCAA943250.020.5137421
GAGTACT461850.020.154612-13
CTGGGGG186000.019.5390515
GGTATCA737350.018.6131131
TCAACGC1045900.018.2379914
CAACGCA1050000.018.1164675
GTACTTT510100.018.09317214-15
ATCAACG1070200.017.799333
TATCAAC1077150.017.7717422
AACGCAG1073900.017.7702146
Produced by FastQC (version 0.11.5)