FastQCFastQC Report
Mon 6 Jun 2016
ERR522999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17254375
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471320.2731597058716992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT419530.24314413011192812No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT274060.15883507805991234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257610.14930126417213024No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG246150.047.713262
GTACCTG292200.040.6785931
ACCTGGG294000.039.336593
CCTGGGG275750.029.482414
GTACATG785000.023.113951
CATGGGG451750.022.9789414
TACATGG810050.022.0931592
CTGGGGG176550.021.8261595
TGGGGGG242250.021.667966
ACATGGG810900.021.3377633
ATGGGGG264350.021.2609675
GTATCAA926700.019.6253031
TATAACG16850.019.5240922
GAGTACT461600.019.45961812-13
GGTATCA734550.018.9917621
AGTACTT493900.017.65404712-13
GTACTTT510600.017.46158814-15
TCAACGC1033250.017.332694
CAACGCA1040450.017.2217275
ATCAACG1054650.016.9765343
Produced by FastQC (version 0.11.5)