FastQCFastQC Report
Mon 6 Jun 2016
ERR522998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9452451
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358030.37876948529011156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289260.3060158682652785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182100.19264844641881773No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125910.13320354688958452No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120550.12753305994392353No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG139150.049.609622
GTACCTG164150.042.7500151
ACCTGGG160850.042.215453
CCTGGGG147300.029.7826064
TATCACG9650.026.8014582
TATAACG9600.025.4715352
GTACATG424800.025.0115181
CATGGGG252150.024.4844044
TACATGG435350.023.9901522
ATGGGGG157350.023.7257045
ACATGGG442100.022.9645253
GTATCAA523950.022.585471
TGGGGGG168400.022.5765466
ATAACGC11950.021.6431313
GGTATCA400300.021.3842561
CTGGGGG96250.020.7612515
GAGTACT308600.020.32061412-13
TAACGCA13050.020.177224
TCAACGC590900.019.7740784
CAACGCA595450.019.5826635
Produced by FastQC (version 0.11.5)