FastQCFastQC Report
Mon 6 Jun 2016
ERR522998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9452451
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309430.3273542491783348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281510.2978169365807874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176590.18681927047281174No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT122320.1294055901479944No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108290.11456287898239303No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG144850.050.6794852
GTACCTG168550.043.9356231
ACCTGGG167850.042.9751743
CCTGGGG156000.031.6899644
TATAACG9000.027.6759822
CTGGGGG91200.023.857055
CATGGGG244250.023.4530724
GTACATG419400.023.2511981
TGGGGGG158300.022.590946
TACATGG431500.022.37122
ATGGGGG156500.022.1901745
GTATCAA532300.021.6938631
ATAACGC10750.021.4199373
ACATGGG436250.021.3069553
GGTATCA408650.021.1820141
TATCACG8350.020.2621562
GTACACG20950.019.9742721
GAGTACT313900.019.52657312-13
TCAACGC600350.018.9583384
TAACGCA12150.018.9517974
Produced by FastQC (version 0.11.5)